Use of biodiesel byproduct crude glycerol as the carbon source for fermentation processes by recombinant Pichia pastoris

Date

2008-05-07

Author

Celik, Eda
Ozbay, Nalan
OKTAR, NURAY
Çalık, Pınar

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

207
views

0
downloads

The aim of this study is to investigate the efficiency of using crude glycerol, the main byproduct of biodiesel industry, directly in the production media of Pichia pastoris fermentation processes. Different biodiesel synthesis conditions were examined, that is, transesterifications of canola, corn, soybean, and sunflower oils, with pure methanol in a molar ratio of 1:6 or 1:3, using 1% or 0.5% (w/v) NaOH as the catalyst. Among these, canola oil-derived glycerol served as the most favorable carbon source, and at a 1:6 molar ratio of canola oil: methanol and C-NaOH = 1% (w/v), the highest recombinant human erythropoietin production (C-rEPO = 31 mg L-1), cell concentration (C-x = 10.5 g L-1), product yield on substrate (Y-rEPO/S = 1.48 mg g(-1)), and cell yield on substrate (Y-X/S = 0.57 g g(-1)) were obtained, having values approximately 1.4-fold higher than those attained with pure glycerol. Thus, this study demonstrated a potential use for crude glycerol, without any purification, in the production of additional value-added products.

Subject Keywords

Industrial and Manufacturing Engineering, General Chemistry, General Chemical Engineering

URI

https://hdl.handle.net/11511/46897

Journal

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH

DOI

https://doi.org/10.1021/ie071613o

Collections

Department of Chemical Engineering, Article

Suggestions

OpenMETU
Core

Effect of dispersed phase feed time on the droplet size of Pickering emulsions produced in a stirred tank Donmez, Dila; Ayrancı Tansık, İnci (Wiley, 2020-06-01) The aim of this study was to investigate the effect of feed time of the oil phase on the average droplet size of Pickering emulsions produced in stirred tanks. Three types of impellers were tested: RT, up-pumping PBT (PBTU), and down-pumping PBT (PBTD). All the impellers were tested at two sizes, T/3 and T/2. All configurations were compared at constant tip speed, power per mass, and impeller Reynolds number. The droplet diameters were measured in Mastersizer (R) 3,000 (Malvern). The results showed that an ...
Effect of Midsynthesis Addition of Silica to the Synthesis Medium on the Properties of MFI-Type Zeolite Membranes Soydas, Belma; Kalıpçılar, Halil; Culfaz, Ali (American Chemical Society (ACS), 2010-06-02) Thin zeolite membranes of MFI type were prepared on alpha-Al2O3 supports by midsynthesis addition of silica in a recirculating flow system at 95 degrees C The synthesis was started with the batch composition of 80SiO(2): 30TPAOH:1500H(2)O which was changed to 80SiO(2)center dot 9TPAOH:1500H(2)O by addition of silica Mkt 24, 48, or 72 h Compared to synthesis at constant composition. it is possible to affect the rates of nucleation and crystal growth to obtain membranes with better quality by changing the ove...
Use of pro scale simulatiors to understand the effects of wettability on miscible carbon dioxide flooding and injectivity Uzun, İlkay; Akın, Serhat; Department of Petroleum and Natural Gas Engineering (2005) This study concentrates on the modelling of three phase flow and miscible CO2 flooding in pore networks that captures the natural porous medium of a reservoir. That is to say, the network, that is a Matlab code, consists of different sided triangles which are located randomly through the grids. The throats that connect the pores are also created by the model. Hence, the lengths and the radii of the throats are varying. The network used in this research is assumed to be representative of mixed-wet carbonates...
Effect of Alkyl Derivatization on Several Properties of N-Halamine Antimicrobial Siloxane Coatings Kocer, Hasan B.; Akdağ, Akın; Ren, Xuehong; Broughton, R. M.; Worley, S. D.; Huang, T. S. (American Chemical Society (ACS), 2008-10-01) Variation of alkyl substitution at position 5 on the hydantoin ring of a series of N-halamine siloxane derivatives has been employed to better understand the biocidal activities of these compounds for use in preparing antimicrobial coatings. The alkyl derivatization of the hydantoin ring at its 5 position, while an essentially constant chlorine loading is maintained at the I position, has shown that there is little dependence of the antimicrobial efficacy against Escherichia coli O157:H7 on the alkyl chain ...
Comparison of Fenitrothion and Trifluralin Adsorption on Organo-Zeolites and Activated Carbon. Part II: Thermodynamic Parameters and the Suitability of the Kinetic Models of Pesticide Adsorption LÜLE ŞENÖZ, Güzide Meltem; Atalay, Mustafa Ümit (Informa UK Limited, 2014-07-04) The suitability of two kinetic models and the thermodynamic parameters of pesticide adsorption were investigated based on obtained data of previous studies. Kinetic evaluation indicated that the pesticides adsorption on adsorbents followed the pseudo-second-order model. Gibbs free energy (Delta G degrees), enthalpy (Delta H degrees), and entropy (Delta S degrees) were calculated for thermodynamic parameters by using linearized Arrhenius equation. The results indicated that the sorption process of fenitrothi...

Citation Formats

E. Celik, N. Ozbay, N. OKTAR, and P. Çalık, “Use of biodiesel byproduct crude glycerol as the carbon source for fermentation processes by recombinant Pichia pastoris,” INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, pp. 2985–2990, 2008, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/46897.