Methanol feeding strategy design enhances recombinant human growth hormone production by Pichia pastoris

Date

2016-03-01

Author

Gunes, Hande
Boy, Erdem
Ata, Ozge
Zerze, Gul H.
Çalık, Pınar
Ozdamar, Tuncer H.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

282
views

0
downloads

BACKGROUND: The effects of designed methanol and co-substrate feeding strategies on fed-batch recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) were investigated.

Subject Keywords

Biochemical engineering, Bioprocesses, Biotechnology, Bioreactors, Fermentation

URI

https://hdl.handle.net/11511/47031

Journal

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

DOI

https://doi.org/10.1002/jctb.4619

Collections

Test and Measurement Center In advanced Technologies (MERKEZ LABORATUVARI), Article

Suggestions

OpenMETU
Core

Fermentation and oxygen transfer characteristics in recombinant human growth hormone production by Pichia pastoris in sorbitol batch and methanol fed-batch operation Çalık, Pınar; Soyaslan, Elif S.; Sahin, Merve; Taspinar, Hatice; Acik, Eda; Bayraktar, Eda (Wiley, 2010-02-01) BACKGROUND: The influence of methanol feed rate on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) in medium containing sorbitol was investigated at three different specific growth rates (mu), namely, 0.02 (MS-0.02), 0.03 (MS-0.03), and 0.04 (MS-0.04).
Exponential feeding strategy development for benzaldehyde lyase production by recombinant "Escherichia coli" Taşpınar, Hatice; Çalık, Pınar; Özdamar, Tunçer H.; Department of Biotechnology (2010) In this study, the aim was to investigate the effects of exponential feeding strategy on benzaldehyde lyase (BAL) production by recombinant Escherichia coli BL21. For this purpose, the effects of medium components were investigated to optimize the initial medium composition of the fed-batch fermentations. For the batch bioreactor operations, the highest cell concentration and BAL activity were achieved in a media containing 30 g L-1 pretreated molasses, and 5 g L-1 (NH4)2HPO4 as 5.07 g L-1, and 1611 U ml-1 ...
Bioprocess design parameters for beta-lactamase production by Bacillus species Çelik, Eda; Çalık, Pınar; Department of Chemical Engineering (2003) In this study, the effects of bioprocess design parameters on β-lactamase production were systematically investigated using wild type Bacillus species. For this purpose, the research programme was carried out in mainly four parts. Initially, potential β-lactamase producers were screened and Bacillus licheniformis ATCC 25972, a constitutive β-lactamase producer, was selected. Next, the effects of bioprocess medium components, i.e., carbon sources (glucose, fructose, sucrose, citric acid and glycerol), inorga...
Recombinant therapeutic protease production by Bacillus sp. Korkmaz, Nuriye; Çalık, Pınar; Department of Chemical Engineering (2007) The first aim of this study is the development of extracellular recombinant therapeutic protease streptokinase producing Bacillus sp., and the second aim is to determine fermentation characteristics for streptokinase production. In this context, the signal (pre-) DNA sequence of B.licheniformis (DSM1969) extracellular serine alkaline protease enzyme gene (subC: Acc. No. X03341) was ligated to 5’ end of the streptokinase gene (skc: Acc. No. S46536) by SOE (Gene Splicing by Overlap Extension) method through P...
Comparison of benzaldehyde lyase production capacity in recombinant Escherichia coli and recombinant Bacillus species Kaya, Hande; Çalık, Pınar; Department of Chemical Engineering (2006) In this study, the benzaldehyde lyase (BAL, EC 4.1.2.38) production in E. coli BL21 (DE3) pLySs as intracellular and in Bacillus species as extracellular were investigated, and comparison of the production capacity of the enzyme in the developed recombinant microorganisms were compared. For this purpose, firstly, PCR amplified bal gene was cloned into pRSETA vector which is under the control of strong T7 promoter and expressed in E. coli BL21 (DE3) pLysS strain. With developed recombinant E. coli BL21 (DE3)...

Citation Formats

H. Gunes, E. Boy, O. Ata, G. H. Zerze, P. Çalık, and T. H. Ozdamar, “Methanol feeding strategy design enhances recombinant human growth hormone production by Pichia pastoris,” JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, pp. 664–671, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/47031.