Selenium containing conducting polymer based pyranose oxidase biosensor for glucose detection

Date

2015-04-01

Author

Gokoglan, Tugba Ceren
SÖYLEMEZ, SANİYE
Kesik, Melis
Toksabay, Sinem
Toppare, Levent Kamil

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

314
views

0
downloads

A novel amperometric pyranose oxidase (PyOx) biosensor based on a selenium containing conducting polymer has been developed for the glucose detection. For this purpose, a conducting polymer; poly(4,7-bis(thieno[3,2-b]thiophen-2-yl)benzo[c][1,2,5] selenadiazole) (poly(BSeTT)) was synthesized via electropolymerisation on gold electrode to examine its matrix property for glucose detection. For this purpose, PyOx was used as the model enzyme and immobilised via physical adsorption technique. Amperometric detection of consumed oxygen was monitored at -0.7 V vs Ag reference electrode in a phosphate buffer (50 mM, pH 7.0). K-M(app), I-max, LOD and sensitivity were calculated as 0.229 mM, 42.37 nA, 3.3 x 10 (4) nM and 6.4 nA/mM cm(2), respectively. Scanning electron microscopy (SEM), Electrochemical Impedance Spectroscopy (EIS) and cyclic voltammetry (CV) techniques were used to monitor changes in surface morphologies and to run electrochemical characterisations. Finally, the constructed biosensor was applied for the determination of glucose in beverages successfully.

Subject Keywords

Amperometric biosensor, Conducting polymer based biosensor, Glucose biosensor, Pyranose oxidase

URI

https://hdl.handle.net/11511/47832

Journal

FOOD CHEMISTRY

DOI

https://doi.org/10.1016/j.foodchem.2014.09.065

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Electrochemical and optical properties of a conducting polymer and its use in a novel biosensor for the detection of cholesterol SÖYLEMEZ, SANİYE; UDUM, YASEMİN; Kesik, Melis; HIZLIATEŞ, CEVHER GÜNDOĞDU; ERGÜN, Mustafa Yavuz; Toppare, Levent Kamil (2015-06-01) A simple and robust cholesterol biosensor was designed by immobilizing cholesterol oxidase (ChOx) onto a conducting polymer modified graphite electrode. For this purpose, monomer, (Z)-4-(4-(9H-carbazol-9-yl) benzylidene) 2 (4 nitrophenyl) oxazol-5(4H)-one (CBNP), was synthesized and electrochemically polymerized on an electrode to achieve an effective immobilization platform for enzyme immobilization. After electropolymerization of the monomer (CBNP), electrochemical and spectroelectrochemical properties we...
A conducting polymer and a calixarene derivative A novel surface design for glucose detection Gökoğlan, Ceren; Söylemez, Saniye; Kesik, Melis; Ünay, Hande; Sayın, Serkan; Çırpan, Ali; Yıldız, Hüseyin Bekir; Toppare, Levent Kamil (null; 2016-07-17) In this study, a novel amperometric glucose biosensor based on a conducting polymer and a calixarene was developed. Conducting polymer of (2‐(2‐oc‐tyldodecyl)‐4,7‐di(selenoph‐2‐yl)‐2H‐benzo[d][1,2,3]triazole)) (SBTz) was used as the immobilization matrix for biomolecule deposition to achieve an effective surface design to detect glucose. After successful deposition of SBTz on graphite electrode, a newly synthesized calixarene and gold nanoparticle (AuNP) mixture were used for improving biosensor character...
Benzodithiophene bearing conjugated polymer-based surface anchoring for sensitive electrochemical glucose detection BULUT, Umut; SAYIN, Vuslat Öykü; Cevher, Sevki Can; Çırpan, Ali; Soylemez, Saniye (2022-10-01) An amino-functionalized, conjugated polymer (P(BDBT)) modified glassy carbon electrode (GCE) was employed as an immobilization platform for glucose oxidase (GOx) enzyme to assemble a novel glucose biosensor. Amino groups available on the polymer backbone served as bioconjugation sites for GOx via glutaraldehyde (GA). The biosensor response to the reduction in oxygen amount because of the enzyme reaction was monitored at –0.7 V potential versus Ag/AgCl. The biosensor displayed a broad linear range between 0....
Fabrication of a promising immobilization platform based on electrochemical synthesis of a conjugated polymer Buber, Ece; SÖYLEMEZ, SANİYE; UDUM, YASEMİN; Toppare, Levent Kamil (2018-07-01) Since conjugated polymers are an important class of materials with remarkable properties in biosensor applications, in this study, a novel glucose biosensor based on a conjugated polymer was fabricated via the electropolymerization of the monomer 10,13-bis(4-hexylthiophen-2-yl)dipyridol[3,2-a:2',3'-c]phenazine onto a graphite electrode surface. Glucose oxidase (GOx) was used as the model biological recognition element. As a result of the enzymatic reaction between GOx and glucose, the glucose amount was det...
Electrochemical polymerization of 1-(4-nitrophenyl)-2,5-di(2-thienyl)-1 H-pyrrole as a novel immobilization platform for microbial sensing Tuncagil, Sevinc; ODACI DEMİRKOL, DİLEK; Varis, Serhat; TİMUR, SUNA; Toppare, Levent Kamil (2009-09-01) Two types of bacterial biosensor were constructed by immobilization of Gluconobacter oxydans and Pseudomonas fluorescens cells on graphite electrodes modified with the conducting polymer; poly(1-(4-nitrophenyl)-2,5-di(2-thienyl)-1 H-pyrrole) [SNS(NO2)]. The measurement was based on the respiratory activity of cells estimated by the oxygen consumption at -0.7 V due to the metabolic activity in the presence of substrate. As well as analytical characterization, the linear detection ranges, effects of electropo...

Citation Formats

T. C. Gokoglan, S. SÖYLEMEZ, M. Kesik, S. Toksabay, and L. K. Toppare, “Selenium containing conducting polymer based pyranose oxidase biosensor for glucose detection,” FOOD CHEMISTRY, pp. 219–224, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/47832.