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Selenium containing conducting polymer based pyranose oxidase biosensor for glucose detection
Date
2015-04-01
Author
Gokoglan, Tugba Ceren
SÖYLEMEZ, SANİYE
Kesik, Melis
Toksabay, Sinem
Toppare, Levent Kamil
Metadata
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A novel amperometric pyranose oxidase (PyOx) biosensor based on a selenium containing conducting polymer has been developed for the glucose detection. For this purpose, a conducting polymer; poly(4,7-bis(thieno[3,2-b]thiophen-2-yl)benzo[c][1,2,5] selenadiazole) (poly(BSeTT)) was synthesized via electropolymerisation on gold electrode to examine its matrix property for glucose detection. For this purpose, PyOx was used as the model enzyme and immobilised via physical adsorption technique. Amperometric detection of consumed oxygen was monitored at -0.7 V vs Ag reference electrode in a phosphate buffer (50 mM, pH 7.0). K-M(app), I-max, LOD and sensitivity were calculated as 0.229 mM, 42.37 nA, 3.3 x 10 (4) nM and 6.4 nA/mM cm(2), respectively. Scanning electron microscopy (SEM), Electrochemical Impedance Spectroscopy (EIS) and cyclic voltammetry (CV) techniques were used to monitor changes in surface morphologies and to run electrochemical characterisations. Finally, the constructed biosensor was applied for the determination of glucose in beverages successfully.
Subject Keywords
Amperometric biosensor
,
Conducting polymer based biosensor
,
Glucose biosensor
,
Pyranose oxidase
URI
https://hdl.handle.net/11511/47832
Journal
FOOD CHEMISTRY
DOI
https://doi.org/10.1016/j.foodchem.2014.09.065
Collections
Department of Chemistry, Article