A functional immobilization matrix based on a conducting polymer and functionalized gold nanoparticles: Synthesis and its application as an amperometric glucose biosensor

Kesik, Melis
Kanik, Fulya Ekiz
Hizalan, Gonul
Kozanoglu, Duygu
Nalbant Esentürk, Emren
Timur, Suna
Toppare, Levent Kamil
Combination of nanoparticles and biomolecules attracted considerable attention in biosensing applications. In this study, effective surface design was investigated by modifying the electrode surface with pristine and functionalized gold nanoparticles. For this purpose, spherical gold nanoparticles were synthesized and characterized with UV-vis spectroscopy and transmission electron microscopy (TEM) analyses. Then, gold nanoparticles were modified with mercaptopropionic acid (MPA) yielding Au-S bonds (Au NPs/MPA). Moreover, a novel functional monomer, 6-(4,7-bis(2,3-dihydrothieno [3,4-b][1,4]dioxin-5-yl)-2H-benzo[d][1,2,3]triazol-2-yl)hexan-1-amine (BEDOA-6), was synthesized and used as an immobilization matrix for glucose biosensor. After successful electrochemical deposition of the polymer; poly(BEDOA-6) on graphite electrodes, immobilization of glucose oxidase (GOx) was carried out covalently with the help of crosslinking agent. During immobilization, Au NPs and Au NPs/MPA were used in biosensor fabrication in order to achieve the most effective surface design for target biosensor. In addition, SEM and fluorescence analyses were utilized to characterize the surface properties. The biosensor shows a wide linear range between 0.025 mM and 1.25 mM glucose concentration with a low detection limit of 0.025 mM. Also, kinetic parameters, operational and storage stabilities were determined. Finally, the biosensor was tested on beverages for glucose detection.


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Two donor-acceptor-donor types of T-conjugated monomers were synthesized using Stille coupling reaction. Both monomers were found to produce electroactive polymers upon electrochemical oxidation. The effects of different donor substituents on the polymers' electrochemical and spectroelectrochemical properties were examined. Optical characterization revealed that the band gaps of poly(2-(2,3-dihydrobenzo[b][1,4]dioxin-6-yl)-3-(2,3-dihydrobenzo[b][1,4]dioxin-7-yl)-5,8-di(thiophen-2-yl)quinoxaline) (PDBQTh) an...
NUGAY, T; KUCUKYAVUZ, S (Wiley, 1992-01-01)
The reaction between 1,5-diethenylnaphthalene and sec-butyllithium produces a new difunctional organolithium initiator which is soluble in non-polar solvents and effective in the synthesis of styrene-isoprene-styrene triblock copolymer. Monomodal molecular weight distributions are observed for block copolymers and styrene homopolymers. The microstructure of polyisoprene blocks is similar to those polymers initiated by butyllithium in the same solvent.
A thiazolothiazole containing multichromic polymer for glucose detection
Soylemez, S.; Kaya, H. Z.; Udum, Y. A.; Toppare, Levent Kamil (Department of Polymer Engineering, Scientific Society of Mechanical Engineering, 2019-10-01)
Donor-acceptor (DA) type monomers namely 2,5-di(thiophen-2-yl)thiazolo[5,4-d]thiazole (TTzTh) and 2,5-bis(3-methylthiophen-2-yl)thiazolo[5,4-d]thiazole (TTzMTh) were synthesized and their electrochemical and optoelectronic properties were investigated in detail. The spectro-electrochemical analysis showed that the alkyl chain substitution results in a shift in the onset of the pi-pi* transition towards longer wavelengths. Depending on the donor substituents, the polymers exhibited optical band gaps 1.65 and...
A new donor-acceptor type polymeric material from a thiophene derivative and its electrochromic properties
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A new electrochromic polymer, poly(2,3,5,8-tetra(thiophen-2-yl)quinoxaline) (PTTQ), was synthesized electrochemically and its electrochromic properties were investigated. The polymer was characterized by Cyclic Voltammetry, Fourier Transform infrared spectroscopy, LTV-Vis-NIR Spectroscopy, and colorimetry. Spectroelectrochemistry analysis demonstrated that the polymer can undergo both p- and true n-type doping processes. The polymer, (PTTQ), has three accessible color states: an oxidized transmissive, a neu...
Soluble Alkyl Substituted Poly(3,4-Propylenedioxyselenophene)s: A New Platform For Optoelectronic Materials
Atak, Samed; Icli-Ozkut, Merve; Önal, Ahmet Muhtar; CİHANER, ATİLLA (Wiley, 2011-10-15)
Optical and electrochemical properties of regiosymmetric and soluble alkylenedioxyselenophene-based electrochromic polymers, namely poly(3,3-dibutyl-3,4-dihydro-2H-seleno pheno [3,4-b][1,4]dioxephine) (PProDOS-C(4)), poly(3,3-dihexyl-3,4-dihydro-2H-selenopheno[3,4-b][1,4]dioxephine) (PProDOS-C(6)), and poly(3,3-didecyl-3,4-dihydro-2H-selenopheno[3,4-b][1,4]dioxephine) (PProDOS-C(10)), are highlighted. It is noted that these unique polymers have low bandgaps (1.57-1.65 eV), and they are exceptionally stable ...
Citation Formats
M. Kesik et al., “A functional immobilization matrix based on a conducting polymer and functionalized gold nanoparticles: Synthesis and its application as an amperometric glucose biosensor,” POLYMER, pp. 4463–4471, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48004.