Low dose dimethyl sulfoxide driven gross molecular changes have the potential to interfere with various cellular processes

Download

10.1038s41598-018-33234-z.pdf

Date

2018-10-04

Author

Tuncer, Sinem
Gurbanov, Rafig
Sheraj, Ilir
Solel, Ege
Esentürk, Okan
Banerjee, Sreeparna

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

123
views

167
downloads

Dimethyl sulfoxide (DMSO) is a small molecule with polar, aprotic and amphiphilic properties. It serves as a solvent for many polar and nonpolar molecules and continues to be one of the most used solvents (vehicle) in medical applications and scientific research. To better understand the cellular effects of DMSO within the concentration range commonly used as a vehicle (0.1-1.5%, v/v) for cellular treatments, we applied Attenuated Total Reflectance (ATR) Fourier Transform Infrared (FT-IR) spectroscopy to DMSO treated and untreated epithelial colon cancer cells. Both unsupervised (Principal Component Analysis-PCA) and supervised (Linear Discriminant Analysis-LDA) pattern recognition/modelling algorithms applied to the IR data revealed total segregation and prominent differences between DMSO treated and untreated cells at whole, lipid and nucleic acid regions. Several of these data were supported by other independent techniques. Further IR data analyses of macromolecular profile indicated comprehensive alterations especially in proteins and nucleic acids. Protein secondary structure analysis showed predominance of beta-sheet over alpha-helix in DMSO treated cells. We also observed for the first time, a reduction in nucleic acid level upon DMSO treatment accompanied by the formation of Z-DNA. Molecular docking and binding free energy studies indicated a stabilization of Z-DNA in the presence of DMSO. This alternate DNA form may be related with the specific actions of DMSO on gene expression, differentiation, and epigenetic alterations. Using analytical tools combined with molecular and cellular biology techniques, our data indicate that even at very low concentrations, DMSO induces a number of changes in all macromolecules, which may affect experimental outcomes where DMSO is used as a solvent.

Subject Keywords

INFRARED-SPECTROSCOPY, ATR-FTIR, DIFFERENTIAL REGULATION, IN-VIVO, C-MYC, CELLS, DMSO, PROTEIN, DIMETHYLSULFOXIDE

URI

https://hdl.handle.net/11511/48089

Journal

SCIENTIFIC REPORTS

DOI

https://doi.org/10.1038/s41598-018-33234-z

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Reduced graphene oxide supported nickel-palladium alloy nanoparticles as a superior catalyst for the hydrogenation of alkenes and alkynes under ambient conditions ÇETİNKAYA, Yasin; Metin, Onder; Balcı, Metin (2016-01-01) Addressed herein is the superior catalytic performance of reduced graphene oxide supported Ni30Pd70 alloy nanoparticles (rGO-Ni30Pd70) for the direct hydrogenation of alkenes and alkynes to alkanes, which surpasses the commercial Pd/C catalyst both in activity and stability. A variety of cyclic or aromatic alkenes and alkynes (a total of 17 examples) were rapidly reduced to the corresponding alkanes with high yields (>99%) via the presented direct hydrogenation protocol under ambient conditions. Compared to...
Supported Ru based ammonia synthesis catalysts Aslan, Mustafa Yasin; Üner, Deniz; Department of Chemical Engineering (2012) Ru/C type ammonia synthesis catalysts are known to be poisoned by hydrogen. In order to elucidate a mechanism for hydrogen poisoning, H2 adsorption and spillover on Ru based ammonia synthesis catalysts were investigated. Supported Ru catalysts and Na promoted Ru catalyst were prepared by incipient wetness impregnation of Ru(NO)(NO3)3 on SiO2, SBA-15, CNT and Vulcan supports. Dispersion value of the catalysts was determined via H2 chemisorption and Transmission Electron Microscopy (TEM) characterization tech...
Low temperature plasma as a means to transform nanoparticle atomic structure Üner, Necip Berker; Thimsen, E. (2018-07-01) Low temperature plasma (LTP) is a highly nonequilibrium substance capable of increasing the specific free energy of mass that flows through it. Despite this attractive feature, there are few examples of the transformation of solid material with an equilibrium atomic structure into a material with a nonequilibrium atomic structure. As a proposed example of such a transformation, in this work, it is argued that the transformation of crystalline metal nanoparticles into amorphous metal nanoparticles is feasibl...
High hydrostatic pressure processing: a method having high success potential in pollen protein extraction ALTUNER, ERGİN MURAT; Ceter, Talip; Alpas, Hami (2012-01-01) Even a single peptide that is present in the pollen wall and cytoplasm could cause pollen allergy. To produce skin-prick test kits, the first step is the extraction of these molecules. In this study, Cedrus atlantica pollens were subjected to 220 and 330 MPa for 10 and 30 min in order to extract these molecules. After high hydrostatic pressure processing (HHPP), the total amounts of proteins (TAPs) are measured and compared with the results of the conventional extraction method (CEM). As a result, the TAPs ...
Increasing clavulanic acid production both in wild type and industrial streptomyces clavuligerus strains by amplification of positive regulator claR gene Mutlu, Alper; Özcengiz, Gülay; Department of Biology (2012) Streptomyces clavuligerus is a Gram-positive, filamentous bacterium which produces several important secondary metabolites, including isopenicillin N, cephamycin C and the β-lactamase inhibitor clavulanic acid. Among these compounds, clavulanic acid is being used in combination with commonly used β-lactam antibiotics in order to fight against bacterial infections that are resistant to such antibiotics. Among these combinations, Augmentin, composed of amoxicillin and clavulanic acid, is the most widely presc...

Citation Formats

S. Tuncer, R. Gurbanov, I. Sheraj, E. Solel, O. Esentürk, and S. Banerjee, “Low dose dimethyl sulfoxide driven gross molecular changes have the potential to interfere with various cellular processes,” SCIENTIFIC REPORTS, pp. 0–0, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48089.