High hydrostatic pressure processing: a method having high success potential in pollen protein extraction

Date

2012-01-01

Author

ALTUNER, ERGİN MURAT
Ceter, Talip
Alpas, Hami

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Even a single peptide that is present in the pollen wall and cytoplasm could cause pollen allergy. To produce skin-prick test kits, the first step is the extraction of these molecules. In this study, Cedrus atlantica pollens were subjected to 220 and 330 MPa for 10 and 30 min in order to extract these molecules. After high hydrostatic pressure processing (HHPP), the total amounts of proteins (TAPs) are measured and compared with the results of the conventional extraction method (CEM). As a result, the TAPs extracted by HHPP is 18.0210 mu g/mL at 220 MPa for 10 min, 22.5770 mu g/mL at 220 MPa for 30 min, 23.3810 mu g/mL at 330 MPa for 10 min and 25.9270 mu g/mL at 330 MPa for 30 min, while this is 1.9460 mu g/mL in 24 h by the CEM. In addition to these results, visual pollen deformation and eruption, pollen wall and surface damage have also been observed.

Subject Keywords

Cedrus atlantica, Atlas cedar, High hydrostatic pressure processing, Pollen protein extraction

URI

https://hdl.handle.net/11511/38995

Journal

HIGH PRESSURE RESEARCH

DOI

https://doi.org/10.1080/08957959.2012.678341

Collections

Department of Food Engineering, Article

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Citation Formats

E. M. ALTUNER, T. Ceter, and H. Alpas, “High hydrostatic pressure processing: a method having high success potential in pollen protein extraction,” HIGH PRESSURE RESEARCH, pp. 291–298, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/38995.