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High hydrostatic pressure processing: a method having high success potential in pollen protein extraction

2012-01-01
ALTUNER, ERGİN MURAT
Ceter, Talip
Alpas, Hami
Even a single peptide that is present in the pollen wall and cytoplasm could cause pollen allergy. To produce skin-prick test kits, the first step is the extraction of these molecules. In this study, Cedrus atlantica pollens were subjected to 220 and 330 MPa for 10 and 30 min in order to extract these molecules. After high hydrostatic pressure processing (HHPP), the total amounts of proteins (TAPs) are measured and compared with the results of the conventional extraction method (CEM). As a result, the TAPs extracted by HHPP is 18.0210 mu g/mL at 220 MPa for 10 min, 22.5770 mu g/mL at 220 MPa for 30 min, 23.3810 mu g/mL at 330 MPa for 10 min and 25.9270 mu g/mL at 330 MPa for 30 min, while this is 1.9460 mu g/mL in 24 h by the CEM. In addition to these results, visual pollen deformation and eruption, pollen wall and surface damage have also been observed.