High hydrostatic pressure processing: a method having high success potential in pollen protein extraction

Ceter, Talip
Alpas, Hami
Even a single peptide that is present in the pollen wall and cytoplasm could cause pollen allergy. To produce skin-prick test kits, the first step is the extraction of these molecules. In this study, Cedrus atlantica pollens were subjected to 220 and 330 MPa for 10 and 30 min in order to extract these molecules. After high hydrostatic pressure processing (HHPP), the total amounts of proteins (TAPs) are measured and compared with the results of the conventional extraction method (CEM). As a result, the TAPs extracted by HHPP is 18.0210 mu g/mL at 220 MPa for 10 min, 22.5770 mu g/mL at 220 MPa for 30 min, 23.3810 mu g/mL at 330 MPa for 10 min and 25.9270 mu g/mL at 330 MPa for 30 min, while this is 1.9460 mu g/mL in 24 h by the CEM. In addition to these results, visual pollen deformation and eruption, pollen wall and surface damage have also been observed.


Effect of high hydrostatic pressure on the profile of proteins extracted from Betula pendula pollens
Altuner, Ergin Murat; Ceter, Talip; Alpas, Hami (2014-10-02)
High hydrostatic pressure (HHP) has high success potential in pollen protein extraction, but its effect on pollen protein profiles has not been studied yet. The aim of this study is to put forward whether HHP processing causes a change in the protein profiles extracted from pollens or not. In this study, proteins extracted from Betula pendula pollens were studied at 100, 200 and 300 MPa at room temperature for 5 min. In addition, the efficiency of three different extraction solvents, namely phosphate buffer...
Low dose dimethyl sulfoxide driven gross molecular changes have the potential to interfere with various cellular processes
Tuncer, Sinem; Gurbanov, Rafig; Sheraj, Ilir; Solel, Ege; Esentürk, Okan; Banerjee, Sreeparna (2018-10-04)
Dimethyl sulfoxide (DMSO) is a small molecule with polar, aprotic and amphiphilic properties. It serves as a solvent for many polar and nonpolar molecules and continues to be one of the most used solvents (vehicle) in medical applications and scientific research. To better understand the cellular effects of DMSO within the concentration range commonly used as a vehicle (0.1-1.5%, v/v) for cellular treatments, we applied Attenuated Total Reflectance (ATR) Fourier Transform Infrared (FT-IR) spectroscopy to DM...
High hydrostatic pressure induced inactivation kinetics of e. coli o157:h7 and s. aureus in carrot juice and analysis of cell volume change
Pilavtepe, Mutlu; Alpas, Hami; Department of Food Engineering (2007)
The main objective of this study was to determine the pressure induced inactivation mechanism of pressure-resistant Escherichia coli O157:H7 933 and Staphylococcus aureus 485 in a low acid food. Firstly, inactivation curves of pathogens were obtained at 200 to 400 MPa at 40ºC in peptone water and carrot juice. First-order and Weibull models were fitted and Weibull model described the inactivation curves of both pathogens more accurately than first-order model, revealing that food systems could exhibit eithe...
High hydrostatic pressure effects on mold flora, citrinin mycotoxin, hydroxytyrosol, oleuropein phenolics and antioxidant activity of black table olives
Tokusoglu, Oezlem; Alpas, Hami; BOZOGLU, Faruk (2010-04-01)
High hydrostatic pressure (HHP) as a non-thermal technology is an effective tool for microbiologically safe and shelf-stable fruits Mycotoxin citrinin (CIT) is a toxic secondary metabolite, especially produced from filamentous fungus Penicillium citrinum and is also produced by other species of Penicillium. Aspergillus, and Monascus that are able to develop on olive after harvest, during brine and storage of olives Nutritional benefits of olive fruit are mainly related to phenolics such as hydroxytyrosol, o...
High performance ligands for the removal of aqueous boron species by continuous polymer enhanced ultrafiltration
YÜRÜM, ALP; TARALP, Alpay; Bicak, Niyazi; Ozbelge, Hilmi Onder; Yılmaz, Levent (Elsevier BV, 2013-07-01)
Boron is an essential element for plant growth, but it may also result in toxicity when present in excessive amounts. In this study, continuous polymer enhanced ultrafiltration (PEUF) was developed to selectively recover aqueous boron species (collectively termed "boron" hereafter) from solution. The PEUF process consisted of complexing boron to a water-soluble polymer, removing all non-complexed solutes by UF, restoring the unloaded polymer, and isolating the newly freed boron by UF. Three different polyme...
Citation Formats
E. M. ALTUNER, T. Ceter, and H. Alpas, “High hydrostatic pressure processing: a method having high success potential in pollen protein extraction,” HIGH PRESSURE RESEARCH, pp. 291–298, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/38995.