Characterization of SDS-degrading Delftia acidovorans and in situ monitoring of its temporal succession in SDS-contaminated surface waters

YILMAZ, Fadime
İçgen, Bülent
Incomplete removal of sodium dodecyl sulfate (SDS) in wastewater treatment plants may result in SDS residues escaping and finding their way into receiving water bodies like rivers, lakes, and sea. Introduction of effective microorganisms into the aerobic treatment facilities can reduce unpleasant by-products and SDS residues. Selecting effective microorganisms for SDS treatment is a big challenge. Current study reports the isolation, identification, and in situ monitoring of an effective SDS-degrading isolate from detergent-polluted river waters. Screening was carried out by the conventional enrichment culture technique and the isolate was tentatively identified by using fatty acid methyl ester and 16S ribosomal RNA (rRNA) sequence analyses. Fatty acids produced by the isolate investigated were assumed as typical for the genus Comamonas. 16S rRNA sequence analysis also confirmed that the isolate had 95 % homology with Delftia acidovorans known as Comamonas or Pseudomonas acidovorans previously. D. acidovorans exhibited optimum growth at SDS concentration of 1 g l(-1) but tolerated up to 10 g l(-1) SDS. 87 % of 1.0 g l(-1) pure SDS was degraded after 11 days of incubation. The temporal succession of D. acidovorans in detergent-polluted river water was also monitored in situ by using Comamonas-specific fluorescein-labeled Cte probe. Being able to degrade SDS and populate in SDS-polluted surface waters, D. acidovorans isolates seem to be very helpful in elimination of SDS.


Use of cadA-Specific Primers and DNA Probes as Tools to Select Cadmium Biosorbents with Potential in Remediation Strategies
İçgen, Bülent (Springer Science and Business Media LLC, 2016-05-01)
Biosorption, using cadmium-resistant bacterial isolates, is often regarded as a relatively inexpensive and efficient way of cleaning up wastes, sediments, or soils polluted with cadmium. Therefore, many efforts have been devoted to the isolation of cadmium-resistant isolates for the efficient management of cadmium remediation processes. However, isolation, identification and in situ screening of efficient cadmium-resistant isolates are primary challenges. To overcome these challanges, in this study, cadA, c...
Diversity of the Aromatic-Ring-Hydroxylating Dioxygenases in the Monoaromatic Hydrocarbon Degraders Held by a Common Ancestor
Yavas, Alper; İçgen, Bülent (Springer Science and Business Media LLC, 2018-09-01)
Aromatic ring hydroxylating dioxygenases (ARHDs), harboured by a variety of bacteria, catalyze the initial reaction in the degradation of a wide range of toxic environmental contaminants like aromatic and polycyclic aromatic hydrocarbons (PAHs). Regardless of the source, bacteria harbouring RHDs play major role in the removal of these toxic contaminants. The diversity of ARHDs in contaminated sites is supposed to be huge. However, most of the ARHD diversity studies are based on the PAH degraders and the ARH...
Levels, Distribution, and Sources of Polychlorinated Biphenyls in Sediments of Lake Eymir, Turkey
Gedik, Kadir; İmamoğlu, İpek (Springer Science and Business Media LLC, 2013-08-01)
Little information is available on the occurrence and fate of persistent organic pollutants in lakes of Turkey. The present study was performed as a first step to assess the occurrence, spatial distribution, and potential sources of polychlorinated biphenyls (PCBs) in surface sediments of Lake Eymir located in a special protection area. I 41PCB pound concentrations range from 1.09 to 2.33 ng g(-1) in 62 sediment samples collected from the lake. The spatial homogeneity of the PCBs along the whole lake sugges...
Tracking transformation processes of organic micropollutants in aquatic environments using multi-element isotope fractionation analysis
Hofstetter, Thomas B.; Bolotin, Jakov; Skarpeli-Liati, Marita; Wijker, Reto; Kurt, Zöhre; Nishino, Shirley F.; Spain, Jim C. (Elsevier BV, 2011-06-01)
The quantitative description of enzymatic or abiotic transformations of man-made organic micropollutants in rivers, lakes, and groundwaters is one of the major challenges associated with the risk assessment of water resource contamination. Compound-specific isotope analysis enables one to identify (bio)degradation pathways based on changes in the contaminants' stable isotope ratios even if multiple reactive and non-reactive processes cause concentrations to decrease. Here, we investigated how the magnitude ...
Evaluation of PCB dechlorination pathways in anaerobic sediment microcosms using an anaerobic dechlorination model
Demirtepe, Hale; Kjellerup, Birthe; Sowers, Kevin R.; İmamoğlu, İpek (Elsevier BV, 2015-10-15)
A detailed quantitative analysis of anaerobic dechlorination (AD) pathways of polychlorinated biphenyls (PCBs) in sediment microcosms was performed by applying an anaerobic dechlorination model (ADM). The purpose of ADM is to systematically analyze changes in a contaminant profile that result from microbial reductive dechlorination according to empirically determined dechlorination pathways. In contrast to prior studies that utilized modeling tools to predict dechlorination pathways, ADM also provides quant...
Citation Formats
F. YILMAZ and B. İçgen, “Characterization of SDS-degrading Delftia acidovorans and in situ monitoring of its temporal succession in SDS-contaminated surface waters,” ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, pp. 7413–7424, 2014, Accessed: 00, 2020. [Online]. Available: