Fed-batch cultivation of bakers' yeast: Effect of nutrient depletion and heat stress on cell composition

Ertugay, N
Hamamci, H
Bayındırlı, Alev
The physiology of a commercial strain of bakers' yeast was studied in terms of the cell composition under different growth conditions and of its response to stress. The study comprised fed-batch experiments since this is the system used in bakers' yeast industry. The classical fed-batch fermentation procedure was modified in that the yeast cells were continuously grown to a steady-state at a dilution rate of 0.1/h in order to achieve more or less the same initial starting point in terms of cell composition. This steady-state culture was then switched to fed-batch concomitantly with exposure to stress. The highest amount of trehalose accumulation was achieved when nutrient depletion and heat stress were applied concomitantly. The highest amount of trehalose, 12 %, was attained in cells stressed by both nitrogen depletion and heat stress. The protein content remained constant, although with some oscillations, at a value of 30 % throughout this dual stress experiment.

Citation Formats
N. Ertugay, H. Hamamci, and A. Bayındırlı, “Fed-batch cultivation of bakers’ yeast: Effect of nutrient depletion and heat stress on cell composition,” FOLIA MICROBIOLOGICA, vol. 42, no. 3, pp. 214–218, 1997, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48547.