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Nutritional and cultural parameters influencing antidipteran delta endotoxin production
Date
2003-01-01
Author
ÖZKAN, MELEK
Dilek, Filiz Bengü
Yetiş, Ülkü
Özcengiz, Gülay
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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In this study, various nutritional and cultural parameters influencing diptera-specific delta-endotoxin synthesis by Bacillus thuringiensis subsp. israelensis HD500 were investigated. Of various inorganic nitrogen sources, the highest yields of Cry11Aa and Cry4Ba proteins were obtained on (NH4)(2)HPO4. Among carbon sources, inulin, dextrin, maltose, lactose, sucrose, whey and glycerol were all stimulatory, while glucose, starch and molasses were suppressive. High concentrations of inorganic phosphate (50 to 100 mM K2HPO4) were required for an effective synthesis of Cry4Ba. Mn was the most critical element for the biosynthesis of both toxins at 10(-6) M concentration. Mg and Ca favored production when provided at 8 x 10(-3) M and 5.5 x 10(-4) M concentrations, respectively, while Fe, Zn and Cu negatively influenced biosynthesis. Cry4-toxin synthesis was best at neutral pH and also when the organism was grown at 25 degreesC. Throughout the study, the extent of growth and sporulation of the producer organism was also monitored. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.
Subject Keywords
Molecular Biology
,
Microbiology
,
General Medicine
URI
https://hdl.handle.net/11511/49154
Journal
Research In Microbiology
DOI
https://doi.org/10.1016/s0923-2508(02)00006-2
Collections
Department of Environmental Engineering, Article
Citation Formats
IEEE
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BibTeX
M. ÖZKAN, F. B. Dilek, Ü. Yetiş, and G. Özcengiz, “Nutritional and cultural parameters influencing antidipteran delta endotoxin production,”
Research In Microbiology
, pp. 49–53, 2003, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/49154.