Trehalose, glycogen and ethanol metabolism in the gcr1 mutant of Saccharomyces cerevisiae.

Since Gcr1p is pivotal in controlling the transcription of glycolytic enzymes and trehalose metabolism seems to be one of the control points of glycolysis, we examined trehalose and glycogen synthesis in response to 2 % glucose pulse during batch growth ingcr1 (glucose regulation-1) mutant lacking fully functional glycolytic pathway and in the wild-type strain. An increase in both trehalose and glycogen stores was observed 1 and 2 h after the pulse followed by a steady decrease in both the wild-type and thegcr1 mutant. The accumulation was faster while the following degradation was slower ingcr1 cells compared to wild-type ones. Although there was no distinct glucose consumption in the mutant cells it seemed that the glucose repression mechanism is similar ingcr1 mutant and in wild-type strain at least with respect to trehalose and glycogen metabolism.
Folia microbiologica


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Aspartokinase is the first enzyme of the aspartate family amino acids biosynthetic pathway. Cephamycin C is a β-lactam antibiotic produced as a secondary metabolite via the enzymatic reactions in the lysine branch of this pathway in Streptomyces clavuligerus. The aspartokinase activity of S. clavuligerus is under concerted feedback inhibition by two of the end product amino acids, lysine plus threonine. It is also known that carbon flow through the lysine branch of the aspartate pathway is rate limiting ste...
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Citation Formats
T. Şeker, “Trehalose, glycogen and ethanol metabolism in the gcr1 mutant of Saccharomyces cerevisiae.,” Folia microbiologica, pp. 193–8, 2003, Accessed: 00, 2020. [Online]. Available: