Trehalose, glycogen and ethanol metabolism in the gcr1 mutant of Saccharomyces cerevisiae.

Date

2003-01-01

Author

Şeker, Tamay

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

141
views

0
downloads

Since Gcr1p is pivotal in controlling the transcription of glycolytic enzymes and trehalose metabolism seems to be one of the control points of glycolysis, we examined trehalose and glycogen synthesis in response to 2 % glucose pulse during batch growth ingcr1 (glucose regulation-1) mutant lacking fully functional glycolytic pathway and in the wild-type strain. An increase in both trehalose and glycogen stores was observed 1 and 2 h after the pulse followed by a steady decrease in both the wild-type and thegcr1 mutant. The accumulation was faster while the following degradation was slower ingcr1 cells compared to wild-type ones. Although there was no distinct glucose consumption in the mutant cells it seemed that the glucose repression mechanism is similar ingcr1 mutant and in wild-type strain at least with respect to trehalose and glycogen metabolism.

Subject Keywords

Microbiology, General Medicine

URI

https://hdl.handle.net/11511/42671

Journal

Folia microbiologica

DOI

https://doi.org/10.1007/bf02930955

Collections

Test and Measurement Center In advanced Technologies (MERKEZ LABORATUVARI), Article

Suggestions

OpenMETU
Core

Cephamycin C production by Streptomyces clavuligerus mutants impaired in regulation of aspartokinase Zeyniyev, Araz; Özcengiz, Gülay; Department of Biotechnology (2006) Aspartokinase is the first enzyme of the aspartate family amino acids biosynthetic pathway. Cephamycin C is a β-lactam antibiotic produced as a secondary metabolite via the enzymatic reactions in the lysine branch of this pathway in Streptomyces clavuligerus. The aspartokinase activity of S. clavuligerus is under concerted feedback inhibition by two of the end product amino acids, lysine plus threonine. It is also known that carbon flow through the lysine branch of the aspartate pathway is rate limiting ste...
Biophysical and microbiological study of high hydrostatic pressure inactivation of Bovine Viral Diarrheavirus type 1 on serum CEYLAN, ÇAĞATAY; Severcan, Feride; ÖZKUL, AYKUT; Severcan, Mete; Bozoglu, Faruk; Taheri, Nusret (Elsevier BV, 2012-01-27) The effect of high hydrostatic pressure application on fetal bovine serum components and the model microorganism (Bovine Viral Diarrheavirus type 1 NADL strain) was studied at 132 and 220 MPa pressure for 5 min at 25 degrees C. Protein secondary structures were found to be unaffected by an artificial neural network application on the amide I region for both untreated and HHP treated samples. FTIR spectroscopy study of both the HHP-treated and control samples revealed changes in the intensity of some bands i...
Fed-batch cultivation of bakers' yeast: Effect of nutrient depletion and heat stress on cell composition Ertugay, N; Hamamci, H; Bayındırlı, Alev (Springer Science and Business Media LLC, 1997-01-01) The physiology of a commercial strain of bakers' yeast was studied in terms of the cell composition under different growth conditions and of its response to stress. The study comprised fed-batch experiments since this is the system used in bakers' yeast industry. The classical fed-batch fermentation procedure was modified in that the yeast cells were continuously grown to a steady-state at a dilution rate of 0.1/h in order to achieve more or less the same initial starting point in terms of cell composition....
Analysis of a bac operon-silenced strain suggests pleiotropic effects of bacilysin in Bacillus subtilis Ertekin, Ozan; Taskin, Ash Aras; Demir, Mustafa; Karataş, Ayten; Özcengiz, Gülay (Springer Science and Business Media LLC, 2020-04-01) Bacilysin, as the simplest peptide antibiotic made up of only L-alanine and L-anticapsin, is produced and excreted by Bacillus subtilis under the control of quorum sensing. We analyzed bacilysin-nonproducing strain OGU1 which was obtained by bacA-targeted pMutin T3 insertion into the parental strain genome resulting in a genomic organization (bacA '::lacZ::erm::bacABCDEF) to form an IPTG-inducible bac operon. Although IPTG induction provided 3- to 5-fold increment in the transcription of bac operon genes, n...
BACILYSIN PRODUCTION BY BACILLUS-SUBTILIS - EFFECTS OF BACILYSIN, PH AND TEMPERATURE Özcengiz, Gülay (Springer Science and Business Media LLC, 1991-01-01) The dipeptide antibiotic bacilysin, when added externally to the early exponential-phase cultures, markedly limited its own synthesis. It was shown in cell-free extracts that the feedback effect does not involve the inhibition of bacilysin synthetase, the enzyme catalyzing bacilysin formation. We also studied pH and temperature dependence of bacilysin production. Production was highest at about pH 6.8 and at 25-degrees-C.

Citation Formats

T. Şeker, “Trehalose, glycogen and ethanol metabolism in the gcr1 mutant of Saccharomyces cerevisiae.,” Folia microbiologica, pp. 193–8, 2003, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/42671.