Immobilization of glucose oxidase onto gelatin for biosensor construction

Date

2005-01-01

Author

Emregul, E
Sungur, S
Akbulut, Ural

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The properties of a glucose biosensor made by immobilization of glucose oxidase onto gelatin in a layer of electrochemically deposited polyaniline have been investigated. Glucose oxidase was immobilized within gelatin cross-links with chromium(III) acetate. The glucose oxidase biosensor was developed by forming a polyaniline-deposited electrode surface as support for the immobilized enzyme gel, in order to increase its durability. The polyaniline/gelatin/glucose oxidase biosensor has been characterized using chemical and electrochemical methods. Temperature, pH, cross-linking agent concentration, enzyme concentration, kinetic properties, reusability and the effect of electro-active compounds were among the parameters studied. The response time of the glucose oxidase biosensor is 90 s, the detection limit is below 1 mmol/dm(3) and the sensor can be used 20 times within a 2-month period without losing its stability.

Subject Keywords

Biophysics, Bioengineering, Biomaterials, Biomedical Engineering

URI

https://hdl.handle.net/11511/48640

Journal

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION

DOI

https://doi.org/10.1163/1568562053700138

Collections

Department of Chemistry, Article

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Citation Formats

E. Emregul, S. Sungur, and U. Akbulut, “Immobilization of glucose oxidase onto gelatin for biosensor construction,” JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, pp. 505–519, 2005, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48640.