In vitro and in vivo evaluation of the effects of demineralized bone matrix or calcium sulfate addition to polycaprolactone-bioglass composites

Date

2010-01-01

Author

ERDEMLİ, ÖZGE
Captug, O.
Bilgili, H.
ORHAN, DİCLEHAN
Tezcaner, Ayşen
Keskin, Dilek

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

214
views

0
downloads

The objective of this study was to improve the efficacy of polycaprolactone/bioglass (PCL/BG) bone substitute using demineralized bone matrix (DBM) or calcium sulfate (CS) as a third component. Composite discs involving either DBM or CS were prepared by compression moulding. Bioactivity of discs was evaluated by energy dispersive X-ray spectroscopy (ESCA) and scanning electron microscopy (SEM) following simulated body fluid incubation. The closest Calcium/Phosphate ratio to that of hydroxyl carbonate apatite crystals was observed for PCL/BG/DBM group (1.53) after 15 day incubation. Addition of fillers increased microhardness and compressive modulus of discs. However, after 4 and 6-week PBS incubations, PCL/BG/DBM discs showed significant decrease in modulus (from 266.23 to 54.04 and 33.45 MPa, respectively) in parallel with its highest water uptakes (36.3 and 34.7%). Discs preserved their integrity with only considerable weight loss (7.5-14.5%) in PCL/BG/DBM group. In vitro cytotoxicity tests showed that all discs were biocompatible. Composites were implanted to defects on rabbit humeri. After 7 weeks, new tissue formation and mineralization at bone-implant interface were observed for all implants. Bone mineral densities at interface were higher than that of implant site and negative controls (defects left empty) but lower than healthy bone level. However, microhardness of implant sites was higher than in vitro results indicating in vivo mineralization of implants. Addition of DBM or CS resulted with higher microhardness values at interface region (ca. 650 mu m from implant) compared to PCL/BG and negative control. Histological studies revealed that addition of DBM enhanced bone formation around and into implant while CS provided cartilage tissue formation around the implant. From these results, addition of DBM or CS could be suggested to improve bone healing efficacy of PCL/BG composites.

Subject Keywords

Biophysics, Bioengineering, Biomaterials, Biomedical Engineering

URI

https://hdl.handle.net/11511/41980

Journal

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE

DOI

https://doi.org/10.1007/s10856-009-3862-6

Collections

Department of Engineering Sciences, Article

Suggestions

OpenMETU
Core

In vitro investigation and biomechanical modeling of the effects of PLF-68 on osteoarthritis in a three-dimensional model Kavas, Aysegul; Ozdemir, Mustafa; Gürses, Senih; Keskin, Dilek; Tezcaner, Ayşen (2011-10-01) In this study, it was hypothesized that Pluronic F-68 (PLF-68) increases matrix synthesis of osteoarthritis (OA) chondrocytes in addition to its well-documented cell survival effect. To test this hypothesis, rat articular chondrocytes were embedded in agarose discs and were exposed to 5-azacytidine (Aza-C) to induce OA-like alterations. Chondrocytes were then treated with PLF-68 (8 and 12 mg/ml) for 10 days. Aza-C-exposed and PLF-68-untreated chondrocytes and Aza-C-unexposed and PLF-68-untreated chondrocyte...
Immobilization of glucose oxidase onto gelatin for biosensor construction Emregul, E; Sungur, S; Akbulut, Ural (Informa UK Limited, 2005-01-01) The properties of a glucose biosensor made by immobilization of glucose oxidase onto gelatin in a layer of electrochemically deposited polyaniline have been investigated. Glucose oxidase was immobilized within gelatin cross-links with chromium(III) acetate. The glucose oxidase biosensor was developed by forming a polyaniline-deposited electrode surface as support for the immobilized enzyme gel, in order to increase its durability. The polyaniline/gelatin/glucose oxidase biosensor has been characterized usin...
In vitro/in vivo comparison of cefuroxime release from poly(epsilon-caprolactone)-calcium sulfate implants for osteomyelitis treatment Yaprakci, Volkan; Erdemli, Ozge; Kayabolen, Alisan; Tezcaner, Ayşen; Bozkurt, Fatih; Keskin, Dilek (Wiley, 2013-11-01) This study aimed to investigate the release of cefuroxime axetil (CF) and calcium from poly(epsilon-caprolactone) (PCL)-calcium sulfate (CaS) implants (PCL:CaS 2:1-10% CF; PCL:CaS 2:1-20% CF; PCL:CaS 1:1-10% CF) for treating infectious bone diseases. Bioactivity, crystallinity and strength, and release profiles under standard and pressurized release conditions were studied. PCL:CaS 2:1-20% CF had slower release than 10% loading. These groups had no significant change in CF and Ca release in response to pres...
Microstructure, microhardness, and biocompatibility characteristics of yttrium hydroxyapatite doped with fluoride Toker, S. M.; Tezcaner, Ayşen; Evis, Zafer (Wiley, 2011-02-01) The current study focused on doping of hydroxyapatite (HA) with constant yttrium (Y3+) and varying fluoride (F-) compositions to investigate its microstructure, microhardness, and biocompatibility. HA was synthesized by precipitation method and sintered at 1100 degrees C for 1 h. Y3+ and F- ion dopings resulted in changes in densities. In x-ray diffraction analysis, no secondary phase formation was observed. Lattice parameters decreased upon ion substitutions. Scanning electron microscopy (SEM) results show...
BOSENTAN, A NON-SPECIFIC ENDOTHELIN ANTAGONIST, STIMULATES FRACTURE HEALING Muratli, Hasan H.; Korkusuz, Feza; KORKUSUZ, PETEK; Bicimoglu, Ali; Ercan, Z. Sevim (National Taiwan University, 2007-02-01) It is assumed that bosentan, a non-selective ET-1 receptor antagonist, will enhance fracture healing. The aim of this prospective randomized controlled study was to investigate the effects of transcutaneous bosentan administration into diaphyseal bone fractures using radiology, histology, prostaglandin E-2 (PGE(2)) and leukotrien C-4 (LTC4) activity measurements.

Citation Formats

Ö. ERDEMLİ, O. Captug, H. Bilgili, D. ORHAN, A. Tezcaner, and D. Keskin, “In vitro and in vivo evaluation of the effects of demineralized bone matrix or calcium sulfate addition to polycaprolactone-bioglass composites,” JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, pp. 295–308, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/41980.