POLYESTER FILM STRIPS COATED WITH PHOTOGRAPHIC GELATIN CONTAINING IMMOBILIZED GLUCOSE-OXIDASE HARDENED BY CHROMIUM(III) SULFATE

Date

1992-01-01

Author

ELCIN, YM
Akbulut, Ural

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Glucose oxidase was immobilized into photographic gelatin hardened by chromium(III) sulphate. The enzyme-gelatin mixture was coated on polyester film strips which allowed easy and simple handling during assays. The effect of gelatin and cross-linker concentrations on water content and enzymatic activity was studied. The effect of pH during immobilization and that of incubation temperature on maximum activity were examined- Enzyme leakage tests were carried out during reuse number studies. Consecutive use of strips followed by washing and resting between uses were found to affect the reuse number. A maximum immobilization of 68% was reached under optimal conditions. Mechanical stability and leakage were found to be functions of gelatin and cross-linker concentrations. Photographic gelatin was found to have many capabilities with extraordinary characteristics as a carrier on immobilization.

Subject Keywords

Biophysics, Mechanics of Materials, Bioengineering, Biomaterials, Ceramics and Composites

URI

https://hdl.handle.net/11511/36960

Journal

BIOMATERIALS

DOI

https://doi.org/10.1016/0142-9612(92)90064-u

Collections

Department of Chemistry, Article

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Citation Formats

Y. ELCIN and U. Akbulut, “POLYESTER FILM STRIPS COATED WITH PHOTOGRAPHIC GELATIN CONTAINING IMMOBILIZED GLUCOSE-OXIDASE HARDENED BY CHROMIUM(III) SULFATE,” BIOMATERIALS, pp. 156–161, 1992, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/36960.