A study of the relative dominance of selected anaerobic sulfate-reducing bacteria in a continuous bioreactor by fluorescence in situ hybridization

Date

2007-01-01

Author

İçgen, Bülent
Harrıson, S.T.L.

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The diversity and the community structure of sulfate-reducing bacteria (SRB) in an anaerobic continuous bioreactor used for treatment of a sulfate-containing wastewater were investigated by fluorescence in situ hybridization. Hybridization to the 16S rRNA probe EUB338 for the domain Bacteria was performed, followed by a nonsense probe NON338 as a control for nonspecific staining. Sulfate-reducing consortia were identified by using five nominally genus-specific probes (SRB129 for Desulfobacter, SRB221 for Desulfobacterium, SRB228 for Desulfotomaculum, SRB660 for Desulfobulbus, and SRB657 for Desulfonema) and four group-specific probes (SRB385 as a general SRB probe, SRB687 for Desulfovibrioaceae, SRB814 for Desulfococcus group, and SRB804 for Desulfobacteriaceae). The total prokaryotic population was determined by 4',6-diamidino-2-phenylindole staining. Hybridization analysis using these 16S rRNA-targeted oligonucleotide probes showed that, of those microbial groupings investigated, Desulfonema, Desulfobulbus, spp., and Desulfobacteriaceae group were the main sulfate-reducing bacteria in the bioreactor when operated at steady state at 35 degrees C, pH 7.8, and a 2.5-day residence time with feed stream containing 2.5 kg m(-3) sulfate as terminal electron acceptor and 2.3 kg m(-3) acetate as carbon source and electron donor.

Subject Keywords

Ecology, Soil Science, Ecology, Evolution, Behavior and Systematics

URI

https://hdl.handle.net/11511/48912

Journal

Microbial Ecology

DOI

https://doi.org/10.1007/s00248-006-9009-0

Collections

Department of Environmental Engineering, Article

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Citation Formats

B. İçgen and S. T. L. Harrıson, “A study of the relative dominance of selected anaerobic sulfate-reducing bacteria in a continuous bioreactor by fluorescence in situ hybridization,” Microbial Ecology, pp. 43–52, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48912.