A study of the relative dominance of selected anaerobic sulfate-reducing bacteria in a continuous bioreactor by fluorescence in situ hybridization

İçgen, Bülent
Harrıson, S.T.L.
The diversity and the community structure of sulfate-reducing bacteria (SRB) in an anaerobic continuous bioreactor used for treatment of a sulfate-containing wastewater were investigated by fluorescence in situ hybridization. Hybridization to the 16S rRNA probe EUB338 for the domain Bacteria was performed, followed by a nonsense probe NON338 as a control for nonspecific staining. Sulfate-reducing consortia were identified by using five nominally genus-specific probes (SRB129 for Desulfobacter, SRB221 for Desulfobacterium, SRB228 for Desulfotomaculum, SRB660 for Desulfobulbus, and SRB657 for Desulfonema) and four group-specific probes (SRB385 as a general SRB probe, SRB687 for Desulfovibrioaceae, SRB814 for Desulfococcus group, and SRB804 for Desulfobacteriaceae). The total prokaryotic population was determined by 4',6-diamidino-2-phenylindole staining. Hybridization analysis using these 16S rRNA-targeted oligonucleotide probes showed that, of those microbial groupings investigated, Desulfonema, Desulfobulbus, spp., and Desulfobacteriaceae group were the main sulfate-reducing bacteria in the bioreactor when operated at steady state at 35 degrees C, pH 7.8, and a 2.5-day residence time with feed stream containing 2.5 kg m(-3) sulfate as terminal electron acceptor and 2.3 kg m(-3) acetate as carbon source and electron donor.
Microbial Ecology


Comparative molecular phylogenetics of Astragalus L. sections from Turkey with New World Astragalus species using nrDNA ITS sequences
Dizkirici, Ayten; EKİCİ, MURAT; Kaya, Zeki (Springer Science and Business Media LLC, 2014-01-01)
Comparative molecular phylogenetic study using nrDNA internal transcribed spacer region (ITS) sequences was carried out on species from three Astragalus L. (Fabaceae) sections from Turkey (Old World species) and the New World. A total of 56 taxa (179 accessions) including 30 species from Incani DC., 15 species from Hypoglottidei DC. and 11 species from Dissitiflori DC. section were used in the current study. The total length of the ITS region was 642 bp with 56 (8.7 %) parsimony-informative and 9 insertion\...
Patterns of dark respiration in aquatic systems
Mantikci, Mustafa; Staehr, Peter A.; Hansen, Jorgen L. S.; Markager, Stiig (CSIRO Publishing, 2020-01-01)
We used continuous measurements of dissolved oxygen (DO) in dark bottles to characterise patterns of the dark respiration rate (R-dark) for three marine phytoplankton monocultures and in natural-water samples from two marine coastal systems. Furthermore, patterns of ecosystem community respiration rate were determined from open-water changes in DO in a fjord and in a lake. We considered two models of R-dark to describe temporal changes in DO: constant R-dark and decreasing R-dark; increasing R-dark. In addi...
An expanded molecular phylogeny of Plumbaginaceae, with emphasis on Limonium (sea lavenders): Taxonomic implications and biogeographic considerations
Koutroumpa, Konstantina; Theodoridis, Spyros; Warren, Ben H.; Jimenez, Ares; Celep, Ferhat; Doğan, Musa; Romeiras, Maria M.; Santos-Guerra, Arnoldo; Maria Fernandez-Palacios, Jose; Caujape-Castells, Juli; Moura, Monica; de Sequeira, Miguel Menezes; Conti, Elena (Wiley, 2018-12-01)
Plumbaginaceae is characterized by a history of multiple taxonomic rearrangements and lacks a broad molecular phylogenetic framework. Limonium is the most species-rich genus of the family with ca. 600 species and cosmopolitan distribution. Its center of diversity is the Mediterranean region, where ca. 70% of all Limonium species are endemic. In this study, we sample 201 Limonium species covering all described infrageneric entities and spanning its wide geographic range, along with 64 species of other Plumba...
Is there any relationship between phytoplankton seasonal dynamics and the carbonate system?
Merico, A; Tyrrell, T; Cokacar, T (Elsevier BV, 2006-01-01)
Production of calcium carbonate by marine calcifying organisms has been shown to decrease under increasing CO2. This effect appears to be driven by a decrease in [CO32-]. The modelling study here described aims at investigating whether the success of a marine calcifying phytoplankton species, the coccolithophore Emiliania huxleyi, may be tied to [CO32-]. The work highlights the complex interactions between the carbonate system variables and spring blooms, and the possibility of a link to the competition bet...
A numerical taxonomic study of the genus Acantholimon Boiss Plumbaginaceae in Ankara Province Turkey
MUVAFFAK, Aslı; Doğan, Musa; Bilgin, Cemal Can (Laser Pages Publishing Ltd., 2001-01-01)
This study examines whether a satisfactory classification of Acantholimon Boiss. (Plumbaginaceae) species found in Ankara province (Turkey) can be obtained from phenctic clustering based on external vegetative and floral morphological characters of both herbarium and collected specimens, The study is on the following species: A. venustum Boiss., A, halophilium Bokhari., A. acerosum (Willd.) Boiss., A. kotschyi (Jaub. & Spach) Boiss., A. confertiflorum Bokhari., A. glumaceum (Jaub. & Spach) Boiss., A. casare...
Citation Formats
B. İçgen and S. T. L. Harrıson, “A study of the relative dominance of selected anaerobic sulfate-reducing bacteria in a continuous bioreactor by fluorescence in situ hybridization,” Microbial Ecology, pp. 43–52, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48912.