Impact of Iontophoresis and PACK-CXL Corneal Concentrations of Antifungals in an In Vivo Model

Yesilirmak, Nilufer
Ozdemir, Huseyin Baran
Unal, Elif Ayca
Erdogan, Merve
Şeker, Tamay
Tum, Atakan Emre
Karakus, Ahmet Kamil
Hizel, Kenan
Bilgihan, Kamil
Purpose: To investigate voriconazole (VRZ) penetration and fungal load in the cornea after applying VRZ therapy with various treatment combinations in a fungal keratitis model. Methods: Fifty-four eyes of 27 young albino rabbits were provided for this experimental study. Twelve corneas were inoculated with Candida albicans, 12 corneas were inoculated with Fusarium solani, and 6 eyes were selected as controls. Infected corneas received various treatment combinations including VRZ 1% drop therapy alone, VRZ 1% plus amphotericin B 1% drop combination therapy, iontophoretic VRZ therapy, and VRZ 1% drop therapy after corneal cross-linking. Fungal load was measured by log reduction, and VRZ levels were quantified by liquid chromatography-tandem mass spectrometry. Results: Iontophoresis-assisted VRZ application showed the highest antifungal activity against F. solani keratitis (4-log reduction) and C. albicans keratitis (5-log reduction) compared with other treatment applications. VRZ levels were also found to be the highest in corneas that received iontophoretic VRZ treatment (3.6313 +/- 0.0990 ppb for F.solani keratitis and 1.7001 +/- 0.0065 ppb for C. albicans keratitis) compared with other treatment applications. Conclusions: Iontophoresis seems to provide the highest VRZ concentration and highest antifungal activity in the cornea compared with other treatment applications for C. albicans and F. solani keratitis.


Variations Of Choroidal And Retinal Thickness Measurements Analyzed By Enhanced Depth Imaging Optical Coherence Tomography In Uveitis
Gurses, Ozlem; Karaismailoglu, Eda (2016-09-01)
Purpose : Choroidal thickness (CT) is considered a promising parameter to monitor the severity of inflammation. We hypothesize that inflammation of the iris and ciliary body in anterior uveitis might affect the CT, and inflammation of the choroid in intermediate, posterior and panuveitis might affect the retinal thickness.(RT) Methods : Prospective case-control study of 32 eyes with anterior, 5 eyes with intermediate, 24 eyes with posterior, 24 eyes with panuveitis, and 51 control eyes were performed with ...
Diagnostic Criteria And Clinical Manifestations Of Presumed Latent Tuberculosis-Related Uveitis In A Bacille Calmette-Guerin Vaccinatinated Community
Gurses, Ozlem; Karaismailoglu, Eda (2015-06-01)
Purpose: The wide range of clinical manifestations of presumed latent tuberculosis-related uveitis (TRU) make its diagnosis difficult in an endemic community. We described the ocular manifestations of patients with TRU, and we evaluated the correlation between skin induration value of tuberculin skin test (TST) and tuberculosis antigens tube value of QuantiFERON®-TB Gold (QFT) test in a Bacille Calmette-Guerin<br /> (BCG) vaccinated community. Methods: This was a prospective 1-year study in a tertiary refe...
Influence of Surface Modifications of Flax Fiber on Mechanical and Flow Properties of Thermoplastic Polyurethane Based Eco-Composites
Tayfun, Umit; DOĞAN, Mehmet; Bayramlı, Erdal (2016-01-01)
In this study, several treatments including alkali, permanganate, peroxide and silanization were applied to flax fiber (FF) to improve its adhesion to bio-based thermoplastic polyurethane (TPU) matrix. TPU/FF composites were prepared at a constant ratio of 30 wt% by using extrusion process. Their mechanical properties, modulus of elasticity, water uptake behaviors, melt-flow properties and morphologies of composites were investigated. All treatments resulted in better mechanical properties with respect to u...
Effect of suppressive DNA on CpG-induced immune activation.
Yamada, H; Gursel, I; Takeshita, F; Conover, J; Ishii, KJ; Gürsel, Mayda; Takeshita, S; Klinman, DM (The American Association of Immunologists, 2002-11-15)
Bacterial DNA and synthetic oligodeoxynucleotides (ODN) containing unmethylated CpG motifs stimulate a strong innate immune response. This stimulation can be abrogated by either removing the CpG DNA or adding inhibitory/suppressive motifs. Suppression is dominant over stimulation and is specific for CpG-induced immune responses (having no effect on LPS- or Con A-induced activation). Individual cells noncompetitively internalize both stimulatory and suppressive ODN. Studies using ODN composed of both stimula...
The serum angiotensin converting enzyme and lysozyme levels in patients with ocular involvement of autoimmune and infectious diseases
Sahin, Ozlem; Ziaei, Alireza; Karaismailoğlu, Eda; Taheri, Nusret (Springer Science and Business Media LLC, 2016-2-16)
Background: Increased serum levels of angiotensin converting enzyme and lysozyme are considered as inflammatory markers for diagnosis of sarcoidosis which is an autoimmune inflammatory disease. The purpose of this study is to evaluate the significance of differences in serum angiotensin converting enzyme and lysozyme levels of patients with ocular involvement of other autoimmune inflammatory and infectious diseases. Methods: This is a prospective study involving patients with ankylosing spondylitis, behc...
Citation Formats
A. KALKANCI et al., “Impact of Iontophoresis and PACK-CXL Corneal Concentrations of Antifungals in an In Vivo Model,” CORNEA, pp. 1463–1467, 2018, Accessed: 00, 2020. [Online]. Available: