Alternative Polyadenylation: Another Foe in Cancer

Download

index.pdf

Date

2016-06-01

Author

Erson Bensan, Ayşe Elif
Can, Tolga

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

161
views

0
downloads

Advancements in sequencing and transcriptome analysis methods have led to seminal discoveries that have begun to unravel the complexity of cancer. These studies are paving the way toward the development of improved diagnostics, prognostic predictions, and targeted treatment options. However, it is clear that pieces of the cancer puzzle are still missing. In an effort to have a more comprehensive understanding of the development and progression of cancer, we have come to appreciate the value of the noncoding regions of our genomes, partly due to the discovery of miRNAs and their significance in gene regulation. Interestingly, the miRNA-mRNA interactions are not solely dependent on variations in miRNA levels. Instead, the majority of genes harbor multiple polyadenylation signals on their 3' UTRs (untranslated regions) that can be differentially selected on the basis of the physiologic state of cells, resulting in alternative 3' UTR isoforms. Deregulation of alternative polyadenylation (APA) has increasing interest in cancer research, because APA generates mRNA 3' UTR isoforms with potentially different stabilities, subcellular localizations, translation efficiencies, and functions. This review focuses on the link between APA and cancer and discusses the mechanisms as well as the tools available for investigating APA events in cancer. Overall, detection of deregulated APA-generated isoforms in cancer may implicate some proto-oncogene activation cases of unknown causes and may help the discovery of novel cases; thus, contributing to a better understanding of molecular mechanisms of cancer. (C) 2016 AACR.

Subject Keywords

Cleavage factor-I, Cytoplasmic poly(A) polymerase, Messenger-Rna Polyadenylation, 3' Untranslated Regions, Large-scale analysis, Structural basis, Gene-expression, Human Tissues, Seq, 3'Utrs

URI

https://hdl.handle.net/11511/49030

Journal

MOLECULAR CANCER RESEARCH

DOI

https://doi.org/10.1158/1541-7786.mcr-15-0489

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Resolving the complexity of the cancer transcriptome through 3’ UTRs Erson Bensan, Ayşe Elif (null; 2017-06-30) Advancements in sequencing and transcriptome analysis methods contributed to a better understanding of the complexity of cancer. These findings are paving the way toward the development of improved diagnostics, prognostic predictions, and targeted treatment options. In an effort to have a more comprehensive understanding of cancer, we focus on the 3’ UTRs (untranslated regions) of genes as we came to appreciate the value of the noncoding regions of our genomes, partly due to microRNAs. The 3’UTRs have long ...
RNA-biology ruling cancer progression? Focus on 3 ' UTRs and splicing Erson Bensan, Ayşe Elif (Springer Science and Business Media LLC, 2020-09-01) The protein-coding regions of mRNAs have the information to make proteins and hence have been at the center of attention for understanding altered protein functions in disease states, including cancer. Indeed, the discovery of genomic alterations and driver mutations that change protein levels and/or activity has been pivotal in our understanding of cancer biology. However, to better understand complex molecular mechanisms that are deregulated in cancers, we also need to look at non-coding parts of mRNAs, i...
Targeted delivery of CPG-oligodeoxynucleotide to breast cancer cells by poly-amidoamine dendrimer-modified magnetic nanoparticles Taghavi Pourianazar, Negar; Gündüz, Ufuk; Gündüz, Güngör; Department of Biotechnology (2016) One major application of nanotechnology in cancer treatment involves designing nanoparticles to deliver drugs, oligonucleotides, and genes to cancer cells. Nanoparticles should be engineered so that they could target and destroy tumor cells with minimal damage to healthy tissues. This research aims to develop an appropriate and efficient nanocarrier, having the ability of interacting with and delivering CpG-oligodeoxynucleotides (CpG-ODNs) to tumor cells. CpG-ODNs activate Toll-like receptor 9 (TLR9), which...
Sequence specific inhibition of human chemokine gene, cxcl7, by small interfering RNAS in lewis lung carcinoma cells Kızışar, Dilay; Akkaya, Mahinur S.; Department of Chemistry (2013) In this study, we aimed to design small interfering RNAs to silence the expression of a gene that is a member of chemokines playing crucial roles on cancer. Small interfering RNAs (siRNAs) are generated by ribonuclease III cleavage of 20-25nt in length dsRNAs which mediate RNAi-induced specific mRNA degradation and they are known to be an important part of the gene regulation mechanism in all Eukaryotes. Recently, siRNAs are being utilized as promising treatment approaches. In this study, we targeted a gene...
Alterations of ceramide/sphingosine 1-phosphate rheostat involved in the regulation of resistance to imatinib-induced apoptosis in K562 human chronic myeloid leukemia cells Baran, Yusuf; Salas, Arelis; Senkal, Can E.; Gündüz, Ufuk; Bielawski, Jacek; Obeid, Lina M.; Ogretmen, Besim (2007-04-13) In this study, mechanisms of resistance to imatinib-induced apoptosis in human K562 cells were examined. Continuous exposure to stepwise increasing concentrations of imatinib resulted in the selection of K562/IMA-0.2 and -1 cells, which expressed similar to 2.3- and 19-fold resistance, respectively. Measurement of endogenous ceramides by high performance liquid chromatography/mass spectroscopy showed that treatment with imatinib increased the generation of ceramide, mainly C-18-ceramide, which is generated ...

Citation Formats

A. E. Erson Bensan and T. Can, “Alternative Polyadenylation: Another Foe in Cancer,” MOLECULAR CANCER RESEARCH, pp. 507–517, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/49030.