Convulsant agent pentylenetetrazol does not alter the structural and dynamical properties of dipalmitoylphosphatidylcholine model membranes

Date

2011-01-25

Author

Turker, Sevgi
Wassall, Stephen
Stillwell, William
Severcan, Feride

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

208
views

0
downloads

Pentylenetetrazol (PTZ) is an epileptogenic agent, which is widely used in the determination of epilepsy-induced alterations and in the assessment of anticonvulsant agents in epileptic studies. Even though PTZ is suggested to induce repetitive firing of nerve fibers and shorten the refractory, its mechanism of action is only partially understood. In the literature there are discrepancies for its action mechanism. While some studies stated that primary sires of PTZ are membrane proteins, some reports indicated that PTZ acts on membrane lipids. In order to pain new insight for this we tested the possibility of interaction of PTZ with a simplified model system called dipalmitoylphosphatidylcholine (DPPC) multilamellar vesicles (MLVs) at agent concentrations (0-24 mol%) using differential scanning calorimetry (DSC), Fourier transform infrared (FTIR), electron spin resonance (ESR) and steady-state fluorescence spectroscopy. The results showed that PTZ at concentrations used (1-24 mol%), does not cause any significant change in lipid phase behavior, lipid dynamics (fluidity), lipid acyl chain flexibility (order), hydration state of the head group and/or the region near the head group of DPPC MLVs. These results clearly revealed that PTZ does not change the structural and dynamical parameters of neutral DPPC lipid vesicles and does not locate within the bilayer.

Subject Keywords

Analytical Chemistry, Spectroscopy, Clinical Biochemistry, Drug Discovery, Pharmaceutical Science

URI

https://hdl.handle.net/11511/49805

Journal

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

DOI

https://doi.org/10.1016/j.jpba.2010.09.002

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

SYNTHESIS AND ANTIFUNGAL ACTIVITY OF SOME NEW BENZIMIDAZOLE DERIVATIVES GOKER, H; ERTAN, R; AKGUN, H; YULUG, N (Wiley, 1991-05-01) Synthesis and antifungal evaluation of 5-ethoxycarbonyl-2-(substituted-benzyl or phenoxymethyl)benzimidazoles are reported. Structures of the compounds were elucidated with IR-, H-1-NMR-, C-13-NMR-, mass-spectra and elemental analysis. Preliminary results show that none of the synthesized benzimidazole derivatives has antifungal activity at the concentration of 100-mu-g/ml against Candida parapsilosis, Candida stellatoidea, and Candida pseudotropicalis.
Reinvestigation of the synthetic and mechanistic aspects of Mn(III) acetate mediated oxidation of enones Demir, Ayhan Sıtkı; Reis, O; Igdir, AC (Elsevier BV, 2004-04-05) Mn(OAc)(3) mediated alpha'-acetoxylation of alpha,beta-unsaturated enones is reinvestigated from a synthetic and mechanistic point of view and an improved procedure based on the use of acetic acid as a co-solvent is presented. Excellent results were obtained for a variety of structurally diverse and synthetically important enones under the optimized conditions.
Dual-adjuvant effect of pH-sensitive liposomes loaded with STING and TLR9 agonists regress tumor development by enhancing Th1 immune response Kocabas, Banu Bayyurt; Almacioglu, Kubra; Bulut, Esin Alpdundar; Gucluler, Gozde; Tincer, Gizem; Bayik, Defne; Gürsel, Mayda; GÜRSEL, İHSAN (Elsevier BV, 2020-12-01) Nucleic acid-based pattern recognition receptor agonists are effective adjuvants and immunotherapeutic agents. Rather than single applications, ligand combinations could synergistically potentiate immune responses by elevating cytokine and chemokine production via triggering multiple signaling pathways. However, short half-lives of such labile ligands due to nuclease attack and limited cellular uptake due to their structure significantly hamper their in vivo performances. More importantly, simultaneous deli...
Asymmetric aldol addition of alpha-azido ketones to ethyl pyruvate mediated by a cinchona-based bifunctional urea catalyst Okumus, Seda; Tanyeli, Cihangir; Demir, Ayhan Sıtkı (Elsevier BV, 2014-07-30) The first asymmetric synthesis of ethyl 4-aryl-3-azido-2-hydroxy-2-methyl-4-oxobutanoates via a cinchona organocatalyst induced aldol addition of alpha-azido ketones to ethyl pyruvate has been developed. The coupling reaction under optimized conditions was carried out to furnish tetrafunctionalized synthons with enantioselectivities of up to 91:9 and enriched diastereoselectivities of up to 95:5 (syn:anti).
The immunological co-adjuvant action of liposomal interleukin-2: The role of mode of localisation of the cytokine and antigen in the vesicles Gürsel, Mayda (Informa UK Limited, 1998-01-01) In experiments designed to study the co-adjuvant action of interleukin-2, a model antigen (tetanus toroid) was passively entrapped in, or covalently coupled to multilamellar liposomes in the presence or absence of interleukin-2 (IL-2). When present, IL-2 was either co-entrapped with the toroid, entrapped alone in liposomes with toroid coupled to their surface, or coupled to the surface of liposomes with entrapped toroid. The role of spatial localization of IL-2 within the liposomal structure (vis a vis that...

Citation Formats

S. Turker, S. Wassall, W. Stillwell, and F. Severcan, “Convulsant agent pentylenetetrazol does not alter the structural and dynamical properties of dipalmitoylphosphatidylcholine model membranes,” JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, pp. 379–386, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/49805.