Genes associated with resistance to wheat yellow rust disease identified by differential display analysis

Bozkurt, Osman
Unver, Turgay
Akkaya, Mahinur
This study is focused on the identification of early response genes involved in the resistance mechanism of one of the most important diseases of wheat, yellow rust. The strategy undertaken was to use differential display reverse transcriptase-PCR method (DDRT-PCR) on two of the yellow rust differential lines of wheat, were infected with the virulent and the avirulent Puccinia striiformis f. sp. tritici races together with appropriate control inoculations. Upon infection of the plant materials (resistant and/or susceptible), different time points were evaluated on DDRT-PCR banding profiles. The expression level differences of 33 genes in DDRT-PCR were further analyzed using quantitative real-time PCR (qRT-PCR). Among which only those (14 gene fragments) resulting reproducible induction or repression levels were reported with measured fold changes. Based on the sequence similarity analysis, all but one were absent in NCBI databank, thus they are newly found wheat sequences. Among those, two genes were identified as full ORF, including 50 and 30 end untranslated regions (UTR); namely cyclophilin like protein (putative antifungal activity) and ubiquitin-conjugating enzyme (E2) or Rad6. The sequence homology analysis of the cloned gene fragments revealed that the genes detected may have roles in ubiquitinylation, programmed cell death (apoptosis), putative antifungal activities, disease resistance responses, pathogenesis related responses, and including a few with no known function.


Bacterial expression of an effector protein of yellow rust pathogen and a resistance protein of wheat and characterization of the effector protein
Erdoğan, Sayıt Mahmut; Akkaya, Mahinur S.; Özçubukçu, Salih; Department of Biotechnology (2019)
Yellow rust is one of the most important wheat disease encountered in large parts of the world and in our country caused by a pathogen which is called as Puccinia striiformis f. sp. tritici (Pst). Rapid alterations in the pathogen virulency can make previously resistant varieties susceptible to the disease. Finding out common or species-specific genes, participated in the plant-pathogen interactions will provide an understanding of the biological mechanisms of the disease. Within the scope of this thesis fo...
Physiological, Biochemical, and Transcriptomic Responses to Boron Toxicity in Leaf and Root Tissues of Contrasting Wheat Cultivars
Kayihan, Ceyhun; Oz, Mehmet Tufan; EYİDOĞAN, FÜSUN; Yucel, Meral; Öktem, Hüseyin Avni (2017-02-01)
In this study, we examined physiological, biochemical, and transcriptomic responses to toxic boron (B) treatment in leaves and roots of two wheat cultivars in order to gain better insight into B response or tolerance mechanisms. Delayed development and reduced vigor caused by high B were not observed in leaves and roots of both cultivars. Length, wet weight, and dry weight were not markedly changed under B toxicity. In leaves, when compared to control, 995 and 892 genes were significantly expressed at least...
Expression profiling in response to ascochyta rabiei inoculations in chickpea
Avcıoğlu Dündar, Banu; Akkaya, Mahinur S.; Department of Biotechnology (2008)
In this study, it was aimed to identify chickpea (Cicer arietinum) genes or gene fragments expressed upon Ascochyta rabiei infection using a tolerant chickpea cultivar ILC195 and fungal isolates with varying level of pathogenicity. PCR amplification of resistance gene analogs (RGA) and disease related genes, and mRNA differential display reverse transcription (DDRT) were used to get these expressed gene fragments in chickpea. The constitutively or differentially expressed PCR product fragments were cloned a...
Genetic screening of Turkish wheat varieties for the durable resistance gene, Lr34
Boylu, Barış; Akkaya, Mahinur S.; Hakkı, Erdoğan Eşref; Department of Biotechnology (2011)
Wheat diseases such as rusts and powdery mildews are among the most important and ancient diseases that affect wheat cultivation worldwide. The pathogen race specific resistance genes cannot maintain long lasting resistance. On the other hand, the presence of genes confers the non-race specific resistance last much longer. The durable resistance phenotypes in wheat against various rust and powdery mildew diseases were reported as Lr34, Yr18, and Pm38 separately; nevertheless, they were known to locate very ...
Bioproduction, structure elucidation and in vitro antiproliferative effect of eumelanin pigment from Streptomyces parvus BSB49
Bayram, Sinan; Dengiz, Çağatay; GERÇEK, Yusuf Can; ÇETİN, İdil; TOPÇUL, Mehmet Rıfkı (Springer Science and Business Media LLC, 2020-01-01)
In this study, the structure of the purified extracellular eumelanin pigment isolated from Streptomyces spp. was elucidated by detailed analysis via two different spectroscopic techniques (FT-IR and NMR). In vitro antiproliferative effects of eumelanin were evaluated on HeLa cell line. These experiments were carried out with the evaluation of the parameters including cell viability, cell index, and mitotic index. With the cell viability and cell index, IC50 concentration of eumelanin was determined as 10 μM...
Citation Formats
O. Bozkurt, T. Unver, and M. Akkaya, “Genes associated with resistance to wheat yellow rust disease identified by differential display analysis,” PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, pp. 251–259, 2007, Accessed: 00, 2020. [Online]. Available: