Tamoxifen Increases Membrane Fluidity at High Concentrations

Date

2000-6-1

Author

Severcan, Feride
Kazanci, Nadide
Zorlu, Faruk

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

193
views

0
downloads

There are contradictory results in the literature relating to the effect of tamoxifen on membrane fluidity. The present work investigates the effect of tamoxifen on membrane dynamics to find out whether the concentration of tamoxifen can be one of the factors in this discrepancy. Turbidity (absorbance at 440 nm) and Fourier transform infrared spectroscopic studies reveal that tamoxifen causes opposite effects on membrane fluidity at low (1 mol.%) and high (30 mol.%) tamoxifen concentrations. Low tamoxifen concentrations increase the absorbance in the gel and liquid crystalline phase, whereas high tamoxifen concentrations decrease the absorbance in gel and liquid crystalline phase, whereas tamoxifen concentrations decrease the absorbance. Observations on both phases show that the bandwidth of the CH2 stretching bands decreases with 1 mol.% tamoxifen and increases with 30 mol.% tamoxifen present, indicating a decrease in membrane fluidity at low tamoxifen concentrations and an increase in fluidity at high tamoxifen concentrations. It is seen that the apparent discrepancy in the literature on the effect of tamoxifen on membrane fluidity mainly arises from the tamoxifen concentration used and the confusion on the concept of lipid fluidity and lipid order.

Subject Keywords

Tamoxifen, Model membrane, Phospholipid membrane, FTIR, Turbidity, Membrane fluidity, Membrane order

URI

https://hdl.handle.net/11511/50882

Journal

Bioscience Reports

DOI

https://doi.org/10.1023/a:1005515502954

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Tamoxifen-model membrane interactions: An FT-IR study Boyar, H; Severcan, Feride (1997-06-01) The temperature- and concentration-induced effects of tamoxifen (TAM) on dipalmitoyl phosphatidylcholine (DPPC) model membranes were investigated by the Fourier transform-infrared (FT IR) spectroscopic technique. An investigation of the C-H stretching region and the C=0 mode reveals that the inclusion of TAM changes the physical properties of the DPPC multibilayers by (i) shifting the main phase transition to lower temperatures; (ii) broadening the transition profile slightly; (iii) disordering the system i...
Immune modulatory effects of pediococcus pentosaceus derived membrane vesicles: mechanism of action and therapeutic applications Alpdündar Bulut, Esin; Gürsel, Mayda; Department of Biology (2018) In our previous studies, we characterized 5 different human gram positive commensal bacteria derived membrane vesicles (MVs) and compared their activity with non-pathogenic E.coli derived membrane vesicles. Results showed that commensal bacteria derived MVs had immunomodulatory properties whereas non-pathogenic E.coli derived membrane vesicles had immune stimulatory properties. In this thesis, we aimed to focus our attention to Pediococcus pentosaceus-derived MVs that displayed the highest immunomodulatory ...
Polycarbonate based zeolite 4a filled mixed matrix membranes: preparation, characterization and gas separation performances Şen, Değer; Yılmaz, Levent; Department of Chemical Engineering (2008) Developing new membrane morphologies and modifying the existing membrane materials are required to obtain membranes with improved gas separation performances. The incorporation of zeolites and low molecular-weight additives (LMWA) into polymers are investigated as alternatives to modify the permselective properties of polymer membranes. In this study, these two alternatives were applied together to improve the separation performance of a polymeric membrane. The polycarbonate (PC) chain characteristics was a...
PERMEABILITY OF PHEMA MEMBRANES PREPARED BY PHOTOINITIATION ARICA, MY; Hasırcı, Vasıf Nejat (1993-01-01) Poly(2-hydroxyethyl methacrylate) membranes were prepared in aqueous media with different ions and ionic strengths. The enzyme glucose oxidase was incorporated into some of these membranes. It was found that membrane water contents and ionic strengths have a linear relationship as long as the salt is not changed. When the salt was varied, only a relationship between salt concentration and water content could be observed. The membrane permeabilities were all found to be of the same order (10(-8) cm2 s-1) but...
PERMEABILITY PROPERTIES OF CHARGED HYDROGEL-CARRYING MEMBRANES AKIN, H; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin (1990-02-01) Poly(2-vinylpyridine) is known to be hydrophobic, and a membrane prepared from it would not permeate water-soluble substances in aqueous media. By introducing hydrophilic poly(2-vinyl-N-methylpyridinium iodide) particles into the membrane, it would be possible to control permeation. This type of membrane was prepared and an increase in permeability was observed on increasing the amount of hydrophilic component in the membrane and decreasing the crosslink density of the hydrogel part. The size of the hydroge...

Citation Formats

F. Severcan, N. Kazanci, and F. Zorlu, “Tamoxifen Increases Membrane Fluidity at High Concentrations,” Bioscience Reports, pp. 177–184, 2000, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/50882.