17 beta-estradiol induced compositional, structural and functional changes in rainbow trout liver, revealed by FT-IR spectroscopy: A comparative study with nonylphenol

Cakmak, G
Togan, İnci Zehra
Severcan, Feride
Steroidal hormones produced by humans and animals are constantly being excreted into the environment. It has been demonstrated that sewage effluent discharged to surface water contains natural estrogens and synthetic estrogenic chemicals. As estrogen levels continuously increase in the aquatic environment, it is very important to have a detailed understanding of estrogens' effects on fish. In the present study, juvenile rainbow trout (Oncorhynchus mykiss) were exposed to 17 beta-estradiol (E2) for 3 weeks and the effects of E2 on rainbow trout livers were investigated at the molecular level using Fourier transform infrared spectroscopy. The results revealed that E2 induced significant alterations in the liver tissues. A decrease in glycogen levels and protein concentration, and an increase in both the population of hepatic lipids, especially triglycerides, as well as the relative content of nucleic acids was observed in the E2 treated liver. In addition, a decrease in the membrane fluidity and an increase in lipid order were found in the cells of treated samples. In order to compare the effect of E2 with that of NP at molecular level, the fish were also treated with an estrogenic compound, nonylphenol (NP). The NP-treated fish liver spectra were found to be quite similar to those of E2-treated fish confirming that NP mimics the effect of E2 in immature rainbow trout.


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Uguz, C; Togan, İnci Zehra; Eroglu, Y; Tabak, I; Zengin, M; İşcan, Mesude (Elsevier BV, 2003-06-01)
Alkylphenol polyethoxylates (APEs) called environmental endocrine disruptors has been shown to accumulate in water around the world. In this study.. the pollution level of alkylphenolic compounds was measured and quantified in water, sediment, and the tissues of fishes collected in two rivers, Sakarya and Degirmendere Rivers, Turkey. Butylphenol (BP) were detected in sediment samples at one sampling stations of both rivers with 1.68 and 3.15 mug/g sediment, while nonylphenol (NP) were detected with the amou...
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Internal release of nutrients is an important contributor to the nutrient dynamics in shallow eutrophic lakes. Zoobenthic organisms may contribute to this release by excreting nutrients to the overlaying water. Based on experiments and using results from previous experimental studies as well as field monitoring density data from 2007 to 2017, we calculated the annual and seasonal nutrient excretions of the two most common macroinvertebrates (Corbicula fluminea and Limnodrilus hoffmeisteri) in Lake Taihu, Ch...
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Ülgüdür, Nilüfer; Bayramoğlu, Tuba Hande; Uludağ-Demirer, Sibel; Demirer, Göksel (Elsevier BV, 2019-09-01)
The effluent stream of the anaerobic digestion processes, the digestate, accommodates high residual organic content that needs to be further treated before discharge. Anaerobic treatment of digestate would not only reduce the residual organic compounds in digestate but also has a potential to capture the associated biogas. High-rate anaerobic reactor configurations can treat the waste streams using lower hydraulic retention times which requires less footprint opposed to the conventional completely stirred t...
Fish and mucus-dwelling bacteria interact to produce a kairomone that induces diel vertical migration in Daphnia
Beklioğlu, Meryem; Gözen, Ayşe Gül (Wiley, 2006-12-01)
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FTIR spectroscopy offers hints towards widespread molecular changes in cobalt-acclimated freshwater bacteria
Kardaş, Mehmet; Gözen, Ayşe Gül; Severcan, Feride (Elsevier BV, 2014-10-01)
High concentrations of heavy metals can be toxic for bacteria. However, after prolonged exposure, bacteria can become acclimated and begin to be able to grow in the presence of heavy metals. Acclimation can involve alterations of metabolism and molecular structures. Our aim was to examine these alterations in cobalt-acclimated bacteria via attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy on viable samples. Bacillus sp. and Pseudomonas sp. isolated from a temperate shallow lake ...
Citation Formats
G. Cakmak, İ. Z. Togan, and F. Severcan, “17 beta-estradiol induced compositional, structural and functional changes in rainbow trout liver, revealed by FT-IR spectroscopy: A comparative study with nonylphenol,” AQUATIC TOXICOLOGY, pp. 53–63, 2006, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/51022.