Human liver steroid sulphotransferase sulphates bile acids

1990-12-15
Radomınska, A.
Comer, Ka
Zimniak, Pawel
Falany, Josie L.
Mesude , Iscan
Charles N. , Falany
The sulphation of bile acids is an important pathway for the detoxification and elimination of bile acids during cholestatic liver disease. A dehydroepiandrosterone (DHEA) sulphotransferase has been purified from male and female human liver cytosol using DEAE-Sepharose CL-6B and adenosine 3′,5′-diphosphate-agarose affinity chromatography [Falany, Vazquez & Kalb (1989) Biochem. J. 260, 641-646]. Results in the present paper show that the DHEA sulphotransferase, purified to homogeneity, is also reactive towards bile acids, including lithocholic acid and 6-hydroxylated bile acids, as well as 3-hydroxylated short-chain bile acids. The highest activity towards bile acids was observed with lithocholic acid (54.3 +/- 3.6 nmol/min per mg of protein); of the substrates tested, the lowest activity was detected with hyodeoxycholic acid (4.2 +/- 0.01 nmol/min per mg of protein). The apparent Km values for the enzyme are 1.5 +/- 0.31 microM for lithocholic acid and 4.2 +/- 0.73 microM for taurolithocholic acid. Lithocholic acid also competitively inhibits DHEA sulphation by the purified sulphotransferase (Ki 1.4 microM). No evidence was found for the formation of bile acid sulphates by sulphotransferases different from the DHEA sulphotransferase during purification work. The above results suggest that a single steroid sulphotransferase with broad specificity encompassing neutral steroids and bile acids exists in human liver.
Biochemical Journal

Suggestions

POSSIBLE INVOLVEMENT OF MANGANESE IN THE CATALYTIC MECHANISM OF BOVINE LIVER ARGINASE
TURKOGLU, S; OZER, I (Elsevier BV, 1992-06-01)
1. Bovine liver arginase followed Michaelis-Menten kinetics in the pH range of 4.5-9.0. The variation of upsilon(i) pH implied that a basic group (pK(alpha) 8.7) functions at the catalytic site.
Context dependent isoform specific PI3K inhibition confers drug resistance in hepatocellular carcinoma cells.
Narci, Kubra; Kahraman, Deniz Cansen; Koyas, Altay; Ersahin, Tulin; Tuncbag, Nurcan; Atalay, Rengul Cetin (2022-03-24)
Background: Targeted therapies for Primary liver cancer (HCC) is limited to the multi-kinase inhibitors, and not fully effective due to the resistance to these agents because of the heterogeneous molecular nature of HCC developed during chronic liver disease stages and cirrhosis. Although combinatorial therapy can increase the efficiency of targeted therapies through synergistic activities, isoform specific effects of the inhibitors are usually ignored. This study concentrated on PI3K/Akt/mTOR pathway and t...
Molecular investigation of therapeutical effects of Ɣ-tocotrienol conjugation therapy with doxorubicin on hepatocellular carcinoma cell line HePG2
Özyurt, İpek; Son, Çağdaş Devrim; Department of Biology (2017)
Hepatocellular carcinoma (HCC) is the fifth leading tumor of the liver tissue in terms of frequency of occurrence and third in terms of mortality. Even though, the most widely used chemotherapeutic agent doxorubicin in treatment of HCC had a great impact for development of therapeutic strategies preventing the progression of tumor formation and elucidating the physiological characteristics of tumor tissue. Moreover, use of doxorubicin minimizes the side effects of the drug in the individual and prevents the...
Large eddy simulation of pressure fluctuations inside stenosed blood vessels towards noninvasive diagnosis of atherosclerosis
Özden, Kamil; Sert, Cüneyt; Yazıcıoğlu, Yiğit; Department of Mechanical Engineering (2018)
Atherosclerosis is a cardiovascular disease, in which plaque builds up inside a blood vessel, narrowing it down and forming a stenosis that adversely affects the flow. Because of the stenosis, turbulent flow occurs at the post-stenotic region, which causespressure fluctuations on the vessel wall. The resulting murmurpropogates through the surrounding tissue and reaches the skin surface. These sounds emitted from the stenosed vessels are evaluated as a sign of stenosis. In this study, large eddy simulations ...
Quantitative expression analysis of the genes potentially involved in clavulanic acid overproduction in streptomyces clavuligerus
Aktaş, Caner; Özcengiz, Gülay; Department of Molecular Biology and Genetics (2018)
Streptomyces clavuligerus is the producer of the medically important β-lactam antibiotics, including cephamycin C (CC) and the potent β-lactamase inhibitor clavulanic acid. (CA). We have already undertaken an extensive comparative proteomic analysis by using both 2-DE-MALDI-MS and GeLC-MS approaches between an industrial CA overproducer, namely DEPA and the reference strain NRRL3585. In this context, we documented several differentially expressed (over- and under-represented) proteins accounting for high CA...
Citation Formats
A. Radomınska, K. Comer, P. Zimniak, J. L. Falany, I. Mesude, and F. Charles N., “Human liver steroid sulphotransferase sulphates bile acids,” Biochemical Journal, pp. 597–604, 1990, Accessed: 00, 2020. [Online]. Available: http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC1149750&blobtype=pdf.