Assessment of genetic variation of bread wheat varieties using microsatellite markers

Akkaya, Mahinur
Buyukunal-Bal, EB
The DNA fingerprinting profiles of 11 bread wheat cultivars, grown in Turkey, was obtained by using 19 highly polymorphic wheat microsatellites. Up to six randomly selected individuals from each of these cultivars were subjected to analysis. Estimated polymorphism information content (PIC) values among 65 individual genotypes for 19 loci were between 0.36 and 0.87 with an average value of 0.68. The numbers of observed alleles were between 2 and 9, with an average value of 5.42. These relationships were assessed amoung cultivars and among their individual genotypes using the mean pairwise average square distance (D-1) (or the delta mu measure of distance (Ddm)) matrix and neighbor-joining method based on 'Nei-72' distance matrix, respectively. Two spring cultivars, 'Kaklic-88' and 'Orso', were closely related to each other; the other 9 winter and facultative cultivars were dispersed. Pedigrees of cultivars represented very little shared information among each other, therefore, they could not been utilized in comparisons.


Assessment of genetic relationships in durum wheat cultivars using AFLP markers
Incirli, A; Akkaya, Mahinur (2001-06-01)
The genetic relationships were assessed for the first time in Turkish durum wheat cultivars using AFLP markers. In the analysis, 18 AFLP primer combinations resulted in a total of 189 polymorphic loci. All of the selective primers used are Eco and Mse primers with three nucleotide extentions on the 3' ends. The number of polymorphic markers per primer combination ranged from 4 to 24. The relationships, among nine winter and six spring type durum wheat cultivars, obtained with various algorithms are in accor...
Assessment of genetic variability for grain nutrients from diverse regions: potential for wheat improvement
Pandey, Anamika; Khan, Mohd Kamran; Hakki, Erdogan E.; Thomas, George; Hamurcu, Mehmet; Gezgin, Sait; Gizlenci, Ozge; Akkaya, Mahinur S. (Springer Science and Business Media LLC, 2016-11-3)
Background: A total of 150 bread wheat genotypes representing 121 Indian and 29 Turkish origin were screened for nutrient concentrations and grain protein content. Elemental and grain protein composition were studied by Inductively Coupled Plasma-Atomic Emission Spectrophotometer and LECO analyser, respectively. The study was performed to determine the variability in nutrient concentrations present in the collected wheat genetic material from two countries. Results: Several fold variations among genotype...
Estimation of Indian and Turkish Hexaploid Wheat Population Structure Employing Molecular Markers
Pandey, Anamika; Khan, Mohd Kamran; Thomas, George; HAKKI, ERDOĞAN EŞREF; KAYIŞ, SEYİT ALİ; HAMURCU, MEHMET; GEZGİN, SAİT; TOPAL, ALİ; Akkaya, Mahinur (2015-01-01)
Bread wheat (Triticum aestivum) is the most commonly grown crop due to its adaptation in a wide range of ecogeographical conditions and providing enhanced food assurance to the modern world. A diverse and rich collection is the foundation of each successful wheat improvement program. Therefore, major efforts are in progress worldwide to boost wheat production by broadening genetic diversity. Accepting this issue as a target, present study gives an overview of the major progress in the diversity and populati...
Characterisation of DNA from archaelogical wheat (Triticum L.) seeds from Anatolia
Somel, Mehmet; Kence, Aykut; Akkaya, Mahinur S.; Department of Biotechnology (2003)
Ancient DNA analysis of archaeological wheat remains may serve to clarify unknown or controversial points in the history of wheat. In the first part of this study, extraction and amplification of DNA from Anatolian charred ancient wheat seeds obtained from different locations and ages was attempted. None of the our extraction samples yielded any PCR amplification. The possible reasons for this result were investigated by constructing an artificial charring experiment. The results suggest that the chances of...
Investigating the roles of micrornas in biotic stress responses and functional characterization of a novel ztl-type f-box protein via virus induced gene silencing
Dağdaş, Yasin Fatih; Akkaya, Mahinur S.; Department of Biotechnology (2009)
Barley and wheat are the two most important crop species in Turkey. Molecular studies for increasing crop yield of these species are very important for the economic benefits of Turkey. Powdery mildew and yellow rust are the two main pathogens, infecting barley and wheat, respectively in our country and causing a great amount of yield loss each year. Till now, classical genetics studies were performed in order to develop resistant barley and wheat cultivars, but these studies have not been succesful. Therefo...
Citation Formats
M. Akkaya and E. Buyukunal-Bal, “Assessment of genetic variation of bread wheat varieties using microsatellite markers,” EUPHYTICA, pp. 179–185, 2004, Accessed: 00, 2020. [Online]. Available: