Assessment of genetic relationships in durum wheat cultivars using AFLP markers

Incirli, A
Akkaya, Mahinur
The genetic relationships were assessed for the first time in Turkish durum wheat cultivars using AFLP markers. In the analysis, 18 AFLP primer combinations resulted in a total of 189 polymorphic loci. All of the selective primers used are Eco and Mse primers with three nucleotide extentions on the 3' ends. The number of polymorphic markers per primer combination ranged from 4 to 24. The relationships, among nine winter and six spring type durum wheat cultivars, obtained with various algorithms are in accordance with the known pedigree information of the cultivars. Based on 'Nei72' genetic distance analysis, the most distant two cultivars are 'Berkmen-469' (winter type) and 'Diyarbakir-88' (spring type), and the closest two are 'Selcuklu-97' and 'Sofu', with the values of 0.793 and 0.115, respectively. The closest two winter type cultivars are `Akbasak-073-44' and `Kunduru-414-44' (0.128).


Assessment of genetic variation of bread wheat varieties using microsatellite markers
Akkaya, Mahinur; Buyukunal-Bal, EB (2004-01-01)
The DNA fingerprinting profiles of 11 bread wheat cultivars, grown in Turkey, was obtained by using 19 highly polymorphic wheat microsatellites. Up to six randomly selected individuals from each of these cultivars were subjected to analysis. Estimated polymorphism information content (PIC) values among 65 individual genotypes for 19 loci were between 0.36 and 0.87 with an average value of 0.68. The numbers of observed alleles were between 2 and 9, with an average value of 5.42. These relationships were asse...
Discriminating durum wheat cultivars using highly polymorphic simple sequence repeat DNA markers
Dograr, N; Akin-Yalin, S; Akkaya, Mahinur (2000-08-01)
The winter type durum wheat varieties of Anatolia used in this study were differentiated for the first time by using simple sequence repeat (SSR) DNA markers or microsatellites. Seven microsatellite markers were used to distinguish four well-adapted landrace selections. five cultivars and seven recently obtained advancing lines. The loci of seven microsatellites were all homozygous, but the WMS6 locus occurred with two alleles in all the genotypes. The genotypes were all distinguished from each other, with ...
Determination of esterase enzyme polymorphism in house fly (Musca domestica L.) populations in Turkey
TAŞKIN, VATAN; Taskin, Belgin Gocmen; KÜÇÜKAKYÜZ, KÖKSAL; Kence, Meral (2011-01-01)
The objective of this study was to determine esterase enzyme polymorphism in 16 natural Musca domestica L. populations from the Aegean and Mediterranean regions of Turkey by using the native polyacrylamide gel electrophoresis (PAGE) technique. The high level of genetic variation among populations and species make esterase enzymes an important tool for analyzing genetic differentiation and evolutionary relationships in insects. In the present study, 22 a-esterase bands were detected by using a-naphthylacetat...
The impact of over-exploitation on the genetic structure of Turkish red pine (Pinus brutia Ten.) populations determined by RAPD markers
Lise, Yildiray; Kaya, Zeki; Isik, Fikret; Sabuncu, Rumi; Kandemir, Irfan; Önde, Sertaç (2007-01-01)
To determine the possible impact of over-exploitation on the genetic structure of Turkish red pine (Pinus brutia Ten.) populations, three natural and three over-exploited (human degraded) populations of the species in the Mediterranean region of Turkey were investigated with Randomly Amplified Polymorphic DNA (RAPD). With the 80 RAPT) primers tested, 12 of them yielded 137 polymorphic RAPD fragments. Four of the studied populations maintained unique fragments. The mean proportion of polymorphic fragments fo...
Assessment of genetic variability for grain nutrients from diverse regions: potential for wheat improvement
Pandey, Anamika; Khan, Mohd Kamran; Hakki, Erdogan E.; Thomas, George; Hamurcu, Mehmet; Gezgin, Sait; Gizlenci, Ozge; Akkaya, Mahinur S. (Springer Science and Business Media LLC, 2016-11-3)
Background: A total of 150 bread wheat genotypes representing 121 Indian and 29 Turkish origin were screened for nutrient concentrations and grain protein content. Elemental and grain protein composition were studied by Inductively Coupled Plasma-Atomic Emission Spectrophotometer and LECO analyser, respectively. The study was performed to determine the variability in nutrient concentrations present in the collected wheat genetic material from two countries. Results: Several fold variations among genotype...
Citation Formats
A. Incirli and M. Akkaya, “Assessment of genetic relationships in durum wheat cultivars using AFLP markers,” GENETIC RESOURCES AND CROP EVOLUTION, pp. 233–238, 2001, Accessed: 00, 2020. [Online]. Available: