Investigating the roles of micrornas in biotic stress responses and functional characterization of a novel ztl-type f-box protein via virus induced gene silencing

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2009

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Dağdaş, Yasin Fatih

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Barley and wheat are the two most important crop species in Turkey. Molecular studies for increasing crop yield of these species are very important for the economic benefits of Turkey. Powdery mildew and yellow rust are the two main pathogens, infecting barley and wheat, respectively in our country and causing a great amount of yield loss each year. Till now, classical genetics studies were performed in order to develop resistant barley and wheat cultivars, but these studies have not been succesful. Therefore, molecular plant-pathogen interactions studies are starting to become the new tool to fight against pathogens. In this thesis, two important aspects of plant microbe interactions were investigated. In the first part, the role of microRNAs (miRNAs) in powdery mildew-barley pathosysytem, and yellow rust-wheat pathosystem were studied. The expression levels of miRNAs and their putative targets were investigated via miRNA microarray analysis and qRT-PCR, respectively, in response to virulent and avirulent pathogen infections. These data were used to establish a new model for powdery mildew-barley and yellow rust-wheat pathosystems. In the second part, functional analysis of a novel F-box gene, which was a ZTL-type F-box, was performed by using Barley Stripe Mosaic Virus mediated Virus Induced Gene Silencing. This F-box gene (HvDRF) (Hordeum vulgare Disease Related F-box) was induced upon yellow rust infection and we studied its role in powdery mildew infection. The results confirmed HvDRF as a positive regulator of race specific immunity and enlarged the roles of ZTL-type F-box proteins to biotic stress responses.

Subject Keywords

Wheat products., Biotechnology.

URI

http://etd.lib.metu.edu.tr/upload/12610641/index.pdf
https://hdl.handle.net/11511/18667

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

Y. F. Dağdaş, “Investigating the roles of micrornas in biotic stress responses and functional characterization of a novel ztl-type f-box protein via virus induced gene silencing,” M.S. - Master of Science, Middle East Technical University, 2009.