Treatment of implant-related methicillin-resistant Staphylococcus aureus osteomyelitis with vancomycin-loaded VK100 silicone cement: An experimental study in rats

2018-1
Neyisci, Cagri
Erdem, Yusuf
Bilekli, Ahmet Burak
Demiralp, Bahtiyar
Kose, Ozkan
Bek, Dogan
Korkusuz, Feza
Kankilic, Berna
Introduction: The purpose of this present study is to investigate the efficacy of vancomycin-loaded VK100 silicone cement drug delivery system in the treatment of implant-related methicillin-resistant Staphylococcus aureus (MRSA) osteomyelitis in rats. Materials and Methods: Thirty-six adult (18-20 weeks old) female Sprague-Dawley rats were included in the study. All rats underwent experimental osteomyelitis surgery via injecting 100 mu L bacterial suspension of MRSA into the medullary canal. After a 2-week duration for the formation of osteomyelitis model, rats were assigned Introduction: randomly into four groups: control (C), systemic vancomycin (V), local vancomycin-loaded VK100 silicone cement (vVK100), and systemic vancomycin and local vancomycin-loaded VK100 silicone cement (V+vVK100). The following treatment protocols were administered to each group for 4 weeks. For group C, 0.9% saline solution equivalent to the volume of vancomycin dose (approximately 1 ml/kg) was administered intraperitoneally twice daily (12-h intervals). For group V, 15 mg/kg of vancomycin was administered intraperitoneally twice daily (12-h intervals). For group vVK100, vVK100 polymer was included so that the intramedullary canal of the rats are affected. For group V+vVK100, vVK100 polymer was included so that the intramedullary canal of the rats are affected and 15 mg/kg of vancomycin was administered intraperitoneally twice daily (12-h intervals). After 4 weeks of treatment, clinical, radiologic, microbiologic, and histopathologic evaluations were performed for all groups. Results: Results of this study revealed that all scores of the evaluation criteria for the treatment groups (groups V, vVK100, and V+vVK100) decreased due to the treatment protocols when compared to group C. These results show the effectiveness of all treatment protocols for the implant-related chronic MRSA osteomyelitis. However, there were no statistical difference between these three protocols. Conclusions: vVK100 polymer, as a local antibiotic delivery system, seems to be an effective method for the treatment of implant-related chronic MRSA osteomyelitis.
Journal of Orthopaedic Surgery

Suggestions

Antioxidant capacities of selected fruits and herbal teas consumed in regular diet and their antimicrobial activities against Staphylococcus aureus
Barut, Burak; Çoruh, Nursen; Department of Biochemistry (2011)
Staphylococcus aureus is one of the major causes of food-borne pathogenesis. Antibiotic consumption for these pathogens has been increasing year by year world-wide. In order to decrease the use of synthetic antibiotics, fresh fruits and dry herbs consumed as beverages in regular diets were examined as potential natural antibiotics for the treatment of food based infections against Staphylococcus aureus. Herbs consumed as tea infusions including Pimpinella anisum L. (anise), Anthemis arvensis L. (camomile), ...
Removal of Pesticides from Wastewater by Membrane Proces
Ateş, Nuray; Uzal, Niğmet; Yetiş, Ülkü; Dilek, Filiz Bengü (2021-06-23)
Purpose: The aim of this study was to investigate the removal performances of four pesticides (tributyl phosphate, flutriafol, dicofol and irgarol) by RO membranes. Methods: Three different RO membranes (BW30-LE, SW30-XLE and GE-AD) were used to reject pesticides in two transmembrane pressures of 10 and 20 bar in bench-scale membrane filtration cell. Tributyl phosphate and flutriafol were detected by GC/MS and dicofol and irgarol were monitored by HLPC instruments. Results: While the highest removal of trib...
Characterization and genomic analysis of a novel bacteriophage against methicillin-resistant staphylococcus aureus
Çotak, Medine; Akkaya, Mahinur S.; Karahan, Zeynep Ceren; Department of Biotechnology (2019)
Methicillin resistant Staphylococcus aureus (MRSA) is one of the most frequent hospital and community acquired infectious agents causing severe diseases. Bacteriophages may offer a solution to treat the bacterial infections that are not responding to classical antibiotics. In this context, a new lytic phage, named as vB_SauM-MikSA913, was isolated from sewage treatment center in Samsun (Turkey). MRSA clinical strains obtained from a local hospital were used as propagating hosts while searching for the lytic...
Treatment of opium alkaloid containing wastewater in sequencing batch reactor (SBR)-Effect of gamma irradiation
Bural, Cavit B.; Demirer, Goksel N.; Kantoglu, Omer; Dilek, Filiz Bengü (Elsevier BV, 2010-04-01)
Aerobic biological treatment of opium alkaloid containing wastewater as well as the effect of gamma irradiation as pre-treatment was investigated. Biodegradability of raw wastewater was assessed in aerobic batch reactors and was found highly biodegradable (83-90% degradation). The effect of irradiation (40 and 140 kGy) on biodegradability was also evaluated in terms of BOD5/COD values and results revealed that irradiation imparted no further enhancement in the biodegradability. Despite the highly biodegrada...
MEMS biosensors for detection of methicillin resistant Staphylococcus aureus
Ceylan Koydemir, Hatice; Külah, Haluk; Özgen, Canan; ALP, ALPASLAN; Hascelik, Gulsen (2011-11-15)
This review presents the current state of the conventional methods, microfluidic based biosensors, and the commercial products used in the detection of methicillin resistant Staphylococcus aureus (MRSA), which is one of the most important threats of nosocomial infections in many parts of the world. The early detection of MRSA in the specimens of the patients is important to enable the appropriate treatment, to decrease morbidity and mortality rates, and to manage control actions in the healthcare units. Thu...
Citation Formats
C. Neyisci et al., “Treatment of implant-related methicillin-resistant Staphylococcus aureus osteomyelitis with vancomycin-loaded VK100 silicone cement: An experimental study in rats,” Journal of Orthopaedic Surgery, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/51720.