Antioxidant capacities of selected fruits and herbal teas consumed in regular diet and their antimicrobial activities against Staphylococcus aureus

Download
2011
Barut, Burak
Staphylococcus aureus is one of the major causes of food-borne pathogenesis. Antibiotic consumption for these pathogens has been increasing year by year world-wide. In order to decrease the use of synthetic antibiotics, fresh fruits and dry herbs consumed as beverages in regular diets were examined as potential natural antibiotics for the treatment of food based infections against Staphylococcus aureus. Herbs consumed as tea infusions including Pimpinella anisum L. (anise), Anthemis arvensis L. (camomile), Rosa canina L. (rosehip), Salvia fruticosa Mill (sage) and fresh fruit juices including Vitis vinifera L. (grape), Citrus sinensis L. (orange), Prunus persica L. (peach) and Punica granatum L. (pomegranate) were selected as samples of hot or cold consumed beverages in our daily diets. Extracts of fresh fruit juices were prepared in methanol, on the other hand, tea infusions of herbs were filtered and lyophilized. Antioxidant capacities of the plant samples were investigated by radical scavenging methods, namely 2’2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) as well as determination of total phenolic and flavonoid contents. Furthermore, antimicrobial activities of plant samples were determined by minimum inhibitory concentration and minimum bactericidal concentration methods along with disc diffusion method. Trolox equivalent antioxidant capacities (TEAC) of the herbal tea infusions obtained by ABTS radical scavenging method were ranged between 48.38 ± 1.242 and 715.73 ± 4.265 (μmol Trolox equivalent (TE)/g of extract) while, TEAC values of fresh fruits juices were between 26.86 ± 0.217 and 73.55 ± 0.973 (μmol Trolox equivalent (TE)/g of extract). Moreover, EC50 values of the tea infusions obtained by DPPH radical scavenging method were ranged between 0.05 ± 0.001 and 1.53 ± 0.004 (mg/mL) while, EC50 values of the fruit juices were 1.6 ± 0.014 and 2 ± 0.093 (mg/mL). Total phenolic content of the plant samples tested in this study were varied from 1.383 (μg gallic acid equivalent (GAE)/mg of extract) to 159.167 (μg gallic acid equivalent (GAE)/mg of extract) and total flavonoid content varied from 0.111 (μg quercetin equivalent (QE)/mg of extract) to 201.15 (μg quercetin equivalent (QE)/mg of extract). Sage, orange and grape displayed higher antimicrobial activities with values of 1.5, 6 and 6 mg/mL minimum inhibitory concentrations and 1.5, 24 and 24 mg/mL minimum bactericidal concentrations, respectively. Inhibition zone diameters of sage, orange and grape were found to be 9, 9 and 11 mm.

Suggestions

Treatment of implant-related methicillin-resistant Staphylococcus aureus osteomyelitis with vancomycin-loaded VK100 silicone cement: An experimental study in rats
Neyisci, Cagri; Erdem, Yusuf; Bilekli, Ahmet Burak; Demiralp, Bahtiyar; Kose, Ozkan; Bek, Dogan; Korkusuz, Feza; Kankilic, Berna (SAGE Publications, 2018-1)
Introduction: The purpose of this present study is to investigate the efficacy of vancomycin-loaded VK100 silicone cement drug delivery system in the treatment of implant-related methicillin-resistant Staphylococcus aureus (MRSA) osteomyelitis in rats. Materials and Methods: Thirty-six adult (18-20 weeks old) female Sprague-Dawley rats were included in the study. All rats underwent experimental osteomyelitis surgery via injecting 100 mu L bacterial suspension of MRSA into the medullary canal. After a 2-week...
Antibiotic loaded nanocapsules functionalized with aptamer gates for targeted destruction of pathogens
Kavruk, M.; ÇELİKBIÇAK, ÖMÜR; Ozalp, V. C.; Borsa, B. A.; Hernandez, F. J.; BAYRAMOĞLU, GÜLAY; SALİH, BEKİR; ARICA, MEHMET YAKUP (2015-01-01)
In this study, we designed aptamer-gated nanocapsules for the specific targeting of cargo to bacteria with controlled release of antibiotics based on aptamer-receptor interactions. Aptamer-gates caused a specific decrease in minimum inhibitory concentration (MIC) values of vancomycin for Staphylococcus aureus when mesoporous silica nanoparticles (MSNs) were used for bacteria-targeted delivery.
Pharmacogenetics of childhood acute lymphoblastic leukemia: investigation of frequency of tpmt risk alleles for thiopurine toxicity and the role of sult1a1, ephx1 polymorphisms as risk factors for development of the disease
Tümer, Tuğba; Arınç, Emel; Department of Biochemistry (2009)
Thiopurine methyltransferase (TPMT) risk alleles (mainly *2,*3B, *3C and *3A) are the major determinants of interindividual differences in the severe toxicity or efficacy of 6-mercaptopurine (6MP) during the treatment of childhood acute lymphoblastic leukemia (ALL). The frequencies of these risk alleles, known to functionally impair TPMT activity, were investigated among 167children with ALL and 206 healthy adult controls in Turkish population by using allele specific PCR and PCRRFLP methods. TPMT*3A and TP...
In vivo interaction of carcinogenic acrylamide with cytochrome p450 isozymes and phase II enzymes in rabbit liver, kidney and lung
Nuyan, Mine; Arınç, Emel; Department of Biochemistry (2008)
Acrylamide is an industrially produced chemical with known neurotoxic, reproductive toxin and carcinogenic effects. The carcinogenicity associated with acrylamide is mostly attributed to its metabolism by liver CYP2E1. However, studies investigating the effects of acrylamide on CYP2E1 enzyme are limited. In this study, it was aimed to investigate in vivo interaction of carcinogenic acrylamide on microsomal cytochrome P450 enzyme activities, and protein levels, and on cytosolic NQO1 and GST enzyme activities...
Antioxidant and Antimicrobial Activities of Essential Oils Obtained from Oregano (Origanum vulgare ssp hirtum) by Using Different Extraction Methods
KARAKAYA, SİBEL; EL, SEDEF NEHİR; Karagozlu, Nural; Şahin, Serpil (Mary Ann Liebert Inc, 2011-06-01)
In this study, antioxidant and antimicrobial activities of essential oils obtained from oregano (Origanum vulgare ssp. hirtum) were determined by using solvent-free microwave extraction (SFME), supercritical fluid extraction, and conventional hydrodistillation (CH) methods. The inhibitory effects on the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical of essential oils obtained from oregano by using SFME and CH were similar. However, essential oil extracted by CH showed greater (2.69 mu mol/mL...
Citation Formats
B. Barut, “Antioxidant capacities of selected fruits and herbal teas consumed in regular diet and their antimicrobial activities against Staphylococcus aureus,” M.S. - Master of Science, Middle East Technical University, 2011.