Antibiotic loaded nanocapsules functionalized with aptamer gates for targeted destruction of pathogens

Kavruk, M.
Ozalp, V. C.
Borsa, B. A.
Hernandez, F. J.
In this study, we designed aptamer-gated nanocapsules for the specific targeting of cargo to bacteria with controlled release of antibiotics based on aptamer-receptor interactions. Aptamer-gates caused a specific decrease in minimum inhibitory concentration (MIC) values of vancomycin for Staphylococcus aureus when mesoporous silica nanoparticles (MSNs) were used for bacteria-targeted delivery.


Antimicrobial aptamers for detection and inhibition of microbial pathogen growth
Ozalp, Veli Cengiz; Bilecen, Kivanc; Kavruk, Murat; Öktem, Hüseyin Avni (Future Medicine Ltd, 2013-03-01)
Discovery of alternative sources of antimicrobial agents are essential in the ongoing battle against microbial pathogens. Legislative and scientific challenges considerably hinder the discovery and use of new antimicrobial drugs, and new approaches are in urgent demand. On the other hand, rapid, specific and sensitive detection of airborne pathogens is becoming increasingly critical for public health. In this respect affinity oligonucleotides, aptamers, provide unique opportunities for the development of na...
Nucleic acid gated nanopore delivery of antibiotics
Kavruk, Murat; Son, Çağdaş Devrim; Özalp, Veli Cengiz; Department of Biotechnology (2018)
Discovery of new strategies in administration of antimicrobial agents are essential in the ongoing battle against pathogens due to rapidly emerging antimicrobial resistance. Nanotechnology provide unique opportunities in this respect for the development of targeted drug delivery solutions for effective usage of current antibiotics. Mesoporous silica particles are micrometer-sized particles with nanometer-sized porous surface structure. They are advantageous molecules due to small size, biocompatibility, wel...
MEMS biosensors for detection of methicillin resistant Staphylococcus aureus
Ceylan Koydemir, Hatice; Külah, Haluk; Özgen, Canan; ALP, ALPASLAN; Hascelik, Gulsen (2011-11-15)
This review presents the current state of the conventional methods, microfluidic based biosensors, and the commercial products used in the detection of methicillin resistant Staphylococcus aureus (MRSA), which is one of the most important threats of nosocomial infections in many parts of the world. The early detection of MRSA in the specimens of the patients is important to enable the appropriate treatment, to decrease morbidity and mortality rates, and to manage control actions in the healthcare units. Thu...
Immunotherapeutic applications of CpG-containing oligodeoxynucleotides
Klinman, DM; Ishii, KJ; Gürsel, Mayda; Gursel, I; Takeshita, S; Takeshita, F (2000-06-01)
Bacterial DNA and synthetic oligodeoxynucleotides (ODN) expressing unmethylated CpG motifs stimulate the mammalian immune system to mount a rapid innate immune response. This response is characterized by the production of polyreactive IgM, immunomodulatory cytokines and chemokines. CpG ODN directly stimulate lymphocytes, natural killer cells and professional antigen-presenting cells (such as macrophages and dendritic cells). Owing to the strength and nature of this stimulation, CpG ODN are being harnessed f...
Immunotherapeutic utility of stimulatory and suppressive oligodeoxynucleotides.
Ishii, KJ; Gursel, I; Gürsel, Mayda; Klinman, DM (2004-04-01)
Bacterial DNA contains immunostimulatory CpG motifs that interact with toll-like receptor 9 on immune cells to stimulate the production of cytokines, chemokines and immunoglobulins. Synthetic oligodeoxynucleotides (ODNs) containing CpG motifs mimic the activity of bacterial DNA. Recently, several structurally distinct types of CpG ODN were identified that differentially activate human immune cells. These ODNs may be useful as vaccine adjuvants, anti-allergens and in the treatment of infectious diseases and ...
Citation Formats
M. Kavruk et al., “Antibiotic loaded nanocapsules functionalized with aptamer gates for targeted destruction of pathogens,” CHEMICAL COMMUNICATIONS, pp. 8492–8495, 2015, Accessed: 00, 2020. [Online]. Available: