FT-IR spectroscopic analysis of rainbow trout liver exposed to nonylphenol

Date

2003-07-01

Author

Cakmak, G
Togan, İnci Zehra
Uguz, C
Severcan, Feride

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

76
views

0
downloads

Nonylphenol (NP) is a biodegradation product of nonylphenol ethoxylates (NPEs) belonging to the alkylphenol ethoxylates (APEs) group. APEs are widely used nonionic surfactants in detergents, herbicides, pesticides, paints, and cosmetics. The present work investigates the effects of NP on rainbow trout (Oncorhynchus mykiss) livers at the molecular level using Fourier transform infrared (FT-IR) spectroscopy. The FT-IR spectra revealed dramatic differences between the NP-treated and control tissues, which mainly indicated that the level of triglycerides increased, the lipid order increased, and the protein concentration decreased in the treated samples. Moreover, it was also found that glycogen levels significantly decreased and the relative content of nucleic acids increased in NP-treated fish. The 17beta-Estradiol-treated fish liver spectra were found to be quite similar to those of NP-treated fish. All these results implied that rainbow trout may offer considerable promise to be used as a bioindicator for NP in the future.

Subject Keywords

Instrumentation, Spectroscopy

URI

https://hdl.handle.net/11511/51934

Journal

APPLIED SPECTROSCOPY

DOI

https://doi.org/10.1366/000370203322102933

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

FTIR spectroscopic analysis of rainbow trout liver exposed to 17 beta-estradiol Severcan, Feride; Togan, İnci Zehra; Cakmak, G (2005-01-01) Nonylphenol (NP) is a biodegradation product of nonylphenol ethoxylates (NPEs) belonging to the alkylphenol ethoxylates (APEs) group. APEs are widely used nonionic surfactants in detergents, herbicides, pesticides, paints, and cosmetics. The present work investigates the effects of NP on rainbow trout (Oncorhynchus mykiss) livers at the molecular level using Fourier transform infrared (FT-IR) spectroscopy. The FT-IR spectra revealed dramatic differences between the NP-treated and control tissues, which main...
Concentration-Based Measurement Studies of L-Tryptophan Using Terahertz Time-Domain Spectroscopy (THz-TDS) Ozer, Zeynep; Gok, Seher; Altan, Hakan; Severcan, Feride (SAGE Publications, 2014-01-01) L-Tryptophan is an extremely important amino acid for a variety of biological functions in living organisms. In this study we were able to measure changes in the concentration of L-tryptophan when incorporated into pellets with polyethylene as a host. The changes were measured both through the characteristic absorption bands of the C11 and C12 bonds in the low terahertz frequency range and using changes in the refractive index where pellets with higher concentrations of L-tryptophan showed higher refractive...
Surface-enhanced Raman of dopamine and neurotransmitters using sol-gel substrates and polymer-coated fiber-optic probes Volkan, Mürvet; Vo-Dinh, T (SAGE Publications, 2000-12-01) Surface-enhanced Raman spectroscopy (SERS) is used to detect catecholamines-viz., dopamine, norepinephrine, epinine, and isoproterenol-using a new substrate prepared with sol-gel technology. Catecholamines are complexed at the surface by iron(III) ions, which were introduced during the reduction of the silver ions embedded inside the sol-gel matrix. With the use of a He-Ne laser at a power of only 12 mW, it is possible to measure dopamine in the micromolar concentration range. A single-fiber SERS probe syst...
Differentiation of mesophilic and thermophilic bacteria with Fourier transform infrared spectroscopy Garip, Sebnem; Bozoglu, Faruk; Severcan, Feride (SAGE Publications, 2007-02-01) In the present study the characterization and differentiation of mesophilic and thermophilic bacteria were investigated by using Fourier transform infrared (FT-IR) spectroscopy. Our results showed significant differences between the FT-IR spectra of mesophilic and thermophilic bacteria. The protein-to-lipid ratio was significantly higher for thermophiles compared to mesophiles. The absorption intensity of the CH3 asymmetric stretching vibration was higher in thermophilic bacteria, indicating a change in the...
FTIR spectroscopic characterization of protein structure in aqueous and non-aqueous media Haris, PI; Severcan, Feride (1999-09-15) With increasing use of proteins in many different applications, ranging from phramaceuticals to biosensors and biomaterials, there has emerged a need for protein structural characterisation in diverse environments. In many cases it is not sufficient to just have the three-dimensional structure of a protein in H2O or in the crystalline state. Often information on the structural properties of a protein is required in the presence of organic solvents, detergent micelles, phospholipid membranes and so on. Fouri...

Citation Formats

G. Cakmak, İ. Z. Togan, C. Uguz, and F. Severcan, “FT-IR spectroscopic analysis of rainbow trout liver exposed to nonylphenol,” APPLIED SPECTROSCOPY, pp. 835–841, 2003, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/51934.