Superoxide dismutase activity in salt stressed wheat seedlings

Eyidogan, F
Öktem, Hüseyin Avni
Yucel, M
We investigated the effect of salt stress on enzymatic activity of superoxide dismutase (SOD) isozymes in shoot and root tissues of salt tolerant and sensitive wheat (Triticum aestivum L. and Triticum durum Defs.) cultivars. Ten day old seedlings were subjected to 0.7 M NaCl stress for 3 and 5 days. Seedlings treated in the same manner without salt stress served as controls. Activity of SOD isozymes in root and shoot extracts was determined by activity staining of native polyacrylamide gels. In both shoot and root extracts of examined cultivars two isozymes of SOD, namely MnSOD and Cu/ZnSOD were identified. Cu/ZnSOD activity comprised 90 % of total SOD activity in both root and shoot tissues. Salt stress caused 1-1.5 fold increase in MnSOD activity of shoots in tolerant cultivars when compared with non-stressed controls. Under stress conditions, compared to controls all cultivars exhibited reduced MnSOD activity in root tissues. Cu/ZnSOD activity, on the other hand, was remarkably enhanced (3-4 fold) in root extracts of the tolerant cultivars, whereas it was reduced in the sensitive ones.


Antioxidant responses of shoots and roots of lentil to NaCl-salinity stress
Bandeoglu, E; Eyidogan, F; Yucel, M; Öktem, Hüseyin Avni (2004-01-01)
The effect of salt stress (100 mM and 200 mM NaCl) on antioxidant responses in shoots and roots of 14-day-old lentil (Lens culinaris M.) seedlings was investigated. Salt stress caused a significant decrease in length, wet-dry weight and an increase in proline content of both shoot and root tissues. In leaf tissues, high salinity treatment resulted in a 4.4 fold increase in H2O2 content which was accompanied by a significant level of lipid peroxidation and an increase in electrolyte leakage. Root tissues wer...
Superoxide dismutase activity of hexaploid and tetraploid wheat cultivars subjected to heat and chilling stress
Eyidogan, F; Öktem, Hüseyin Avni; Yucel, M (2003-01-01)
Effect of heat (37degreesC, 24 hours) and chilling (4degreesC, 24 hours) stress on SOD activity was investigated in shoots and roots of hexaploid and tetraploid wheat cultivars. In order to analyse the changes of SOD isozymes against heat and chilling stress, shoot and root extracts were subjected to native PAGE. Extracts of both tissues revealed the presence of Mn and Cu/Zn isozymes of SOD. Cu/ZnSOD activity comprised 90% of total SOD activity in both tissues. Heat stress caused a significant increase in S...
Biochemical analysis of trehalose and its metabolizing enzymes in wheat under abiotic stress conditions
El-Bashiti, T; Hamamcı, Haluk; Öktem, Hüseyin Avni; Yucel, M (2005-07-01)
In this study, three wheat cultivars (Triticum aestivum L.) Tosun, Bolal (stress tolerant) and Cakmak (stress sensitive) were analysed for the presence of trehalose. Using gas chromatography-mass spectrometry (GC-MS) analysis, trehalose was unambiguously identified in extracts from seeds and seedlings of different wheat cultivars. The trehalose amount was quantified by high performance liquid chromatography (HPLC) connected with refractive index detector. Effects of drought and salt stress on trehalose cont...
Effect of Protein-Lipid-Salt Interactions on Sodium Availability in the Mouth and Consequent Perception of Saltiness: In Solutions
Yücel, Umut; Peterson, Devin G. (2015-09-02)
The influence of protein sodium interactions on the availability of sodium in the aqueous phase of liquid samples and consequently on the perception of saltiness was investigated. The aqueous effluents of casein and casein emulsion salt solutions were monitored for sodium availability from a tongue column system. In the aqueous protein salt solutions, increasing the protein/salt ratio from 1:1 to 5:1 or 10:1 significantly decreased the initial salt concentration in the effluent and resulted in a higher salt...
Generating salt-tolerant Nicotiana tabacum and identification of stress-responsive miRNAs in transgenics
AYSIN, Ferhunde; Erson Bensan, Ayşe Elif; Eyidogan, Fusun; Öktem, Hüseyin Avni (2015-01-01)
Identification of vacuolar Na+/H+ antiporters facilitates the basis of salt stress tolerance mechanisms. Na+ accumulation into the vacuole is crucial for the avoidance of cytoplasmic Na+ toxicity. In this study, we show that the introduction of AtNHX1 into tobacco generated more tolerant plants when compared to wild-type plants. Transgenic tobacco plants exhibited higher germination rates in the presence of increasing salt concentrations compared to wild-type plants. In addition, proline levels were higher ...
Citation Formats
F. Eyidogan, H. A. Öktem, and M. Yucel, “Superoxide dismutase activity in salt stressed wheat seedlings,” ACTA PHYSIOLOGIAE PLANTARUM, pp. 263–269, 2003, Accessed: 00, 2020. [Online]. Available: