Superoxide dismutase activity of hexaploid and tetraploid wheat cultivars subjected to heat and chilling stress

Eyidogan, F
Öktem, Hüseyin Avni
Yucel, M
Effect of heat (37degreesC, 24 hours) and chilling (4degreesC, 24 hours) stress on SOD activity was investigated in shoots and roots of hexaploid and tetraploid wheat cultivars. In order to analyse the changes of SOD isozymes against heat and chilling stress, shoot and root extracts were subjected to native PAGE. Extracts of both tissues revealed the presence of Mn and Cu/Zn isozymes of SOD. Cu/ZnSOD activity comprised 90% of total SOD activity in both tissues. Heat stress caused a significant increase in SOD activity both in root and shoot tissues of the hexaploid cultivars whereas a decrease was observed in the tetraploid cultivar. Under cold stress no significant change in SOD activity was observed in shoot tissues of all cultivars. On the contrary, SOD activities in the roots of the hexaploid cultivars were significantly enhanced while a decrease was observed in the roots of tetraploid cultivar. Our data suggest that enhanced SOD activity might be one of the reasons of relatively greater stress tolerance of bread wheat cultiars over durum cultivars under heat and chilling stress conditions.


Superoxide dismutase activity in salt stressed wheat seedlings
Eyidogan, F; Öktem, Hüseyin Avni; Yucel, M (2003-01-01)
We investigated the effect of salt stress on enzymatic activity of superoxide dismutase (SOD) isozymes in shoot and root tissues of salt tolerant and sensitive wheat (Triticum aestivum L. and Triticum durum Defs.) cultivars. Ten day old seedlings were subjected to 0.7 M NaCl stress for 3 and 5 days. Seedlings treated in the same manner without salt stress served as controls. Activity of SOD isozymes in root and shoot extracts was determined by activity staining of native polyacrylamide gels. In both shoot a...
Antioxidant responses of shoots and roots of lentil to NaCl-salinity stress
Bandeoglu, E; Eyidogan, F; Yucel, M; Öktem, Hüseyin Avni (2004-01-01)
The effect of salt stress (100 mM and 200 mM NaCl) on antioxidant responses in shoots and roots of 14-day-old lentil (Lens culinaris M.) seedlings was investigated. Salt stress caused a significant decrease in length, wet-dry weight and an increase in proline content of both shoot and root tissues. In leaf tissues, high salinity treatment resulted in a 4.4 fold increase in H2O2 content which was accompanied by a significant level of lipid peroxidation and an increase in electrolyte leakage. Root tissues wer...
Biochemical analysis of trehalose and its metabolizing enzymes in wheat under abiotic stress conditions
El-Bashiti, T; Hamamcı, Haluk; Öktem, Hüseyin Avni; Yucel, M (2005-07-01)
In this study, three wheat cultivars (Triticum aestivum L.) Tosun, Bolal (stress tolerant) and Cakmak (stress sensitive) were analysed for the presence of trehalose. Using gas chromatography-mass spectrometry (GC-MS) analysis, trehalose was unambiguously identified in extracts from seeds and seedlings of different wheat cultivars. The trehalose amount was quantified by high performance liquid chromatography (HPLC) connected with refractive index detector. Effects of drought and salt stress on trehalose cont...
Antioxidant responses of lentil to cold and drought stress
Öktem, Hüseyin Avni; Demirba, Didem; Bayrac, Abdullah Tahir; Oez, Mehmet Tufan; Oezguer, Ebru; Selcuk, Feyza; Yuecel, Meral (2008-01-01)
The effects of cold and drought stress on antioxidant responses and growth parameters in shoots and roots of lentil (Lens culinaris M cv Sultan 1) seedlings were investigated. Ten-day-old hydroponically grown seedlings were subjected to drought and cold (4 degrees C) stress for 5 days. The length and fresh weight of shoots decreased significantly under both stress conditions, contrary to the increase in these growth parameters for roots under the same conditions. The oxidative damage as:generation of malond...
Cu/Zn superoxide dismutase activity and respective gene expression during cold acclimation and freezing stress in barley cultivars
Kayihan, C.; EYİDOĞAN, FÜSUN; Afsar, N.; Öktem, Hüseyin Avni; YÜCEL, MUSTAFA (Institute of Experimental Botany, 2012-12-01)
The transcript levels and activities of the superoxide dismutase isoenzyme (Cu/ZnSOD) were assessed in winter (Tarm-92) and spring (Zafer-160) barley cultivars during cold acclimation, freezing stress and after rewarming. Leaf Cu/ZnSOD activity and Cu/ZnSOD expression level were not significantly changed during cold acclimation. The Cu/ZnSOD expression increased evidently at mild freezing stress (-3 A degrees C; F1), while Cu/ZnSOD1 activity did not show any response and Cu/ZnSOD2 activity decreased continu...
Citation Formats
F. Eyidogan, H. A. Öktem, and M. Yucel, “Superoxide dismutase activity of hexaploid and tetraploid wheat cultivars subjected to heat and chilling stress,” CEREAL RESEARCH COMMUNICATIONS, pp. 387–394, 2003, Accessed: 00, 2020. [Online]. Available: