Development of a laccase based paper biosensor for the detection of phenolic compounds

Date

2012-04-01

Author

Öktem, Hüseyin Avni
EYİDOĞAN, FÜSUN
Bayrac, Ceren
Yilmaz, Remziye

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

253
views

0
downloads

Laccase (E.C.1.10.3.2, p-benzenediol: oxygen oxidoreductase) catalyzes the oxidation of various aromatic compounds, particularly phenols, which are organic pollutants, present in wastewater. With this specific function this enzyme has had a great impact on the development of biosensors for both environmentally important pollutants and clinically relevant metabolites. There are different detection procedures such as chromatographic and spectrophotometric methods to detect the presence of phenolic compounds in wastewater. However, they are time-consuming processes and require specific instruments and qualified staff. The development of a low-cost, portable and disposable biosensor by the immobilization of laccase in Whatman paper has been described. The enzyme is loaded with colouring agent MBTH that allows detection of oxidation products of phenols by forming maroon-green colours. The optimum enzyme concentration loaded onto papers forming lowest background colour and producing highest colour intensity was determined to be 2 mg/ml while the optimum MBTH concentration was 24 mM. The biosensor was responsive to L-DOPA and catechol, with the lowest detection limits of 64 mu M. The colour intensities were found to be consistent with substrate concentration and the results were reproducible.

Subject Keywords

Biosensors, Laccase, MBTH, Phenols

URI

https://hdl.handle.net/11511/53168

Journal

JOURNAL OF FOOD AGRICULTURE & ENVIRONMENT

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Development of new synthetic methodologies for the synthesis of unusual isocoumarin and indole derivatives:The chemistry of homophthalic acid Özcan, Sevil; Balcı, Metin; Department of Chemistry (2007) Many heterocyclic compounds containing nitrogen, oxygen and sulfur show wide range of physiological activities and their synthesis has always been attracted the interest of chemists. The aim of this research is to develop new synthetic methodologies leading to the synthesis of new derivatives of isocoumarines, indoles, isoquinolines, benzodiazepinones and quinazolines, which have been found to show important biological activities. Starting from homophthalic acid and bishomophthalic acid the corresponding ac...
Development of novel Ag/bauxite nanocomposite as a heterogeneous catalyst for biodiesel production Bet-Moushoul, Elsie; Farhadi, Khalil; Mansourpanah, Yaghoub; Molaie, Rahim; Forough, Mehrdad; Nikbakht, Ali Mohammad (2016-07-01) Ag/bauxite nanocomposites have been prepared using in situ reduction of aqueous AgNO3 solution in a bauxite matrix and investigated for the transesterification of sunflower oil with methanol in order to study their potential as heterogeneous catalysts. The prepared nanocopmosites were characterized by XRD, SEM, EDX, FT-IR, and TG- DTA. The Central Composite Design of the Response Surface Methodology was used to optimize the effect of reaction temperature, reaction time, catalyst loading and methanol to oil ...
Development of new synthetic methodologies for indole derivatives Kılıklı, Ahmet Alper; Balcı, Metin; Department of Chemistry (2010) Synthesizing nitrogen containing heterocyclic compounds is one of the leading research areas throughout the organic chemistry due to their significant activities on biological systems. Among the various biologically active molecules, indole derivatives are of prime importance on the grounds of their proven clinical roles. Objective of this study is to synthesize new indole derivatives those may contribute treatment of several diseases like their analogues via a recently developed synthetic methodology. Besi...
Development of polycarbonate based zeolite 4A filled mixed matrix gas separation membranes SEN, Deger; Kalıpçılar, Halil; Yılmaz, Levent (2007-10-15) The incorporation of fillers, blending with multifunctional low molecular-weight additives and their combination are investigated as alternatives for modifying the permselective properties of polymeric gas separation membranes. For this purpose, pure polycarbonate (PC), PC/p-nitroaniline (pNA), PC/zeolite 4A and PC/pNA/zeolite 4A mixed matrix membranes were prepared by solvent-evaporation method using dichloromethane as solvent. Zeolite 4A was the filler and pNA was the low molecular-weight additive with mu...
Development of a New Biosensor by Adsorption of Creatinine Deiminase on Monolayers of Micro- and Nanoscale Zeolites Marchenko, Svitlana; Piliponskiy, Ihor I.; Mamchur, Olha O.; Soldatkin, Oleksandr O.; Kucherenko, Ivan S.; Kasap, Berna Ozansoy; Akata Kurç, Burcu; Dzyadevych, Sergei; Soldatkin, Alexei P. (2016-08-27) This work is dedicated to the development of creatinine-sensitive biosensor consisting of pH-sensitive field-effect transistor (pH-FET) and creatinine deiminase (CD) immobilized with various types of zeolites, in particular, silicalite, zeolite beta (BEA) and nanobeta, and BEA zeolites, modified with gold nanoparticles and ions. For comparison, the traditional method of CD immobilization in saturated glutaraldehyde (GA) vapor was used. To modify pH-FET with zeolites, a monolayer method of deposition was app...

Citation Formats

H. A. Öktem, F. EYİDOĞAN, C. Bayrac, and R. Yilmaz, “Development of a laccase based paper biosensor for the detection of phenolic compounds,” JOURNAL OF FOOD AGRICULTURE & ENVIRONMENT, pp. 1030–1034, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/53168.