MODULATION OF CYTOCHROME P450s AND ANTIOXIDANT ENZYMES BY CAFFEIC ACID, p-COUMARIC ACID AND QUERCETIN-RICH ANATOLIAN PROPOLIS IN HUMAN ADENOCARCINOMA HT-29 CELLS

Date

2018-01-01

Author

ALTAY, AHMET
İrtem Kartal, Deniz
Sagdicoglu-Celep, Gulcin
Güray, Nülüfer Tülün
Bozoglu, Faruk

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Propolis, which is a complex resinous material has diverse pharmacological and biological properties. Phase I and II enzymes are responsible for detoxication and elimination of activated carcinogens, acting as important biomarkers for chemoprevention. Among them, cytochrome P450s plays a prominent role in metabolic activation of xenobiotics. This study was designed to investigate the in vitro modulatory effects of Anatolian propolis and its phenolic contents; caffeic acid, quercetin and p-coumaric acid on the mRNA expression levels of CYP1A1, CYP1A2, CYP2E1, NQOI, GSTP1 and GPx in human colorectal adenocarcinoma HT-29 cells. Analysis results revealed that the propolis extract and its major phenolic contents; caffeic acid and quercetin suppressed the mRNA expression levels of CYP1A1, CYP1A2 and CYP2E1 between the range of 1,66 and 4,55 fold, while p-coumaric acid did not show remarkable effects on the enzymes aforementioned. On the other hand, the extract and all phenolics increased the mRNA expression levels of antioxidant enzymes; NQO1, GSTP1 and GPx between the range of 1,51 and 9,85 fold. This preliminary study demonstrates that phenolic content rich-Anatolian propolis modulates the gene expression of the enzymes involved in xenobiotics activation and detoxication pathways.

Subject Keywords

Propolis, Phenolics, Cytotoxicity, Phase I and II enzymes

URI

https://hdl.handle.net/11511/53313

Journal

FRESENIUS ENVIRONMENTAL BULLETIN

Collections

Department of Biology, Article

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Citation Formats

A. ALTAY, D. İrtem Kartal, G. Sagdicoglu-Celep, N. T. Güray, and F. Bozoglu, “MODULATION OF CYTOCHROME P450s AND ANTIOXIDANT ENZYMES BY CAFFEIC ACID, p-COUMARIC ACID AND QUERCETIN-RICH ANATOLIAN PROPOLIS IN HUMAN ADENOCARCINOMA HT-29 CELLS,” FRESENIUS ENVIRONMENTAL BULLETIN, pp. 336–345, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/53313.