MODULATION OF CYTOCHROME P450s AND ANTIOXIDANT ENZYMES BY CAFFEIC ACID, p-COUMARIC ACID AND QUERCETIN-RICH ANATOLIAN PROPOLIS IN HUMAN ADENOCARCINOMA HT-29 CELLS

Date

2018-01-01

Author

ALTAY, AHMET
İrtem Kartal, Deniz
Sagdicoglu-Celep, Gulcin
Güray, Nülüfer Tülün
Bozoglu, Faruk

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

196
views

0
downloads

Propolis, which is a complex resinous material has diverse pharmacological and biological properties. Phase I and II enzymes are responsible for detoxication and elimination of activated carcinogens, acting as important biomarkers for chemoprevention. Among them, cytochrome P450s plays a prominent role in metabolic activation of xenobiotics. This study was designed to investigate the in vitro modulatory effects of Anatolian propolis and its phenolic contents; caffeic acid, quercetin and p-coumaric acid on the mRNA expression levels of CYP1A1, CYP1A2, CYP2E1, NQOI, GSTP1 and GPx in human colorectal adenocarcinoma HT-29 cells. Analysis results revealed that the propolis extract and its major phenolic contents; caffeic acid and quercetin suppressed the mRNA expression levels of CYP1A1, CYP1A2 and CYP2E1 between the range of 1,66 and 4,55 fold, while p-coumaric acid did not show remarkable effects on the enzymes aforementioned. On the other hand, the extract and all phenolics increased the mRNA expression levels of antioxidant enzymes; NQO1, GSTP1 and GPx between the range of 1,51 and 9,85 fold. This preliminary study demonstrates that phenolic content rich-Anatolian propolis modulates the gene expression of the enzymes involved in xenobiotics activation and detoxication pathways.

Subject Keywords

Propolis, Phenolics, Cytotoxicity, Phase I and II enzymes

URI

https://hdl.handle.net/11511/53313

Journal

FRESENIUS ENVIRONMENTAL BULLETIN

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

The role of the cross pathway control protein in the stress response and adaptation of aspergillus species to antifungals Amarsaikhan, Nansalmaa; Ögel, Zümrüt Begüm; Bakır, Ufuk; Department of Biotechnology (2010) In this study, the adaptation and response of Aspergillus nidulans and Aspergillus fumigatus wild type and cpcA strains to antifungal compounds were studied using cultural, genetic and proteomic methods. CpcA is the fungal cross pathway control protein which may also have a role in the development of resistance to antifungal that has become a major problem in human and plant fungal diseases and many studies are devoted to address the drug resistance mechanisms. Cell adapts itself to stress when it is subje...
Monitoring of Tryptophan as a Biomarker for Cancerous Cells in Terahertz (THz) Sensing Altan, Hakan; Gok, Seher; Ozyurt, Ipek; Severcan, Feride (2016-02-17) Tryptophan is an extremely important amino acid for a variety of biological functions in living organisms. Changes in the concentration of this amino acid can point to identification of cancerous tissues or even confirm symptoms of depression in patients. Therefore it is extremely important to identify and quantify tryptophan concentrations in human blood as well as in in-vivo diagnostic studies. Here a reflection based terahertz pulsed spectroscopy system was used to study the interaction of THz pulses wit...
Modulation of mRNA expression and activities of xenobiotic metabolizing enzymes, CYP1A1, CYP1A2, CYP2E1, GPx and GSTP1 by the Salicornia freitagii extract in HT-29 human colon cancer cells ALTAY, AHMET; İrtem Kartal, Deniz; SADİ, GÖKHAN; Güray, Nülüfer Tülün; YAPRAK, AHMET EMRE (2017-01-01) Phase I-II detoxification and antioxidant enzymes are responsible for the detoxification and elimination of activated carcinogens, acting as important biomarkers for chemoprevention. Among them, cytochrome P450s plays a prominent role in the metabolic activation of xenobiotics. The herb Salicornia freitagii (SF) (Amaranthaceae) is known for its anticancer, antioxidant, antidiabetic and antiinflammatory activities. In this study, we determined the bioactive phenolics in the SF methanol extract and investigat...
AN ONCOGENIC ISOFORM SWITCH LEADS TO HNRNPA1 OVEREXPRESSION IN TRIPLE NEGATIVE BREAST CANCER Erdem, Murat; Erson Bensan, Ayşe Elif; Department of Biology (2022-8-05) HNRNPA1 is one of the most abundant and ubiquitously expressed nuclear proteins and plays a significant role in RNA biology. It has many diverse functions in cellular nucleic acid metabolisms, including mRNA transport, miRNA maturation, and telomere maintenance. Emerging evidence suggest HNRNPA1 to be an important RNA binding protein (RBP) to regulate various RNA related processes in cancer cells. However, deregulation of HNRNPA1 expression and its action mechanisms are unclear in breast cancers. The study...
Changes in gene profile of human hepatocellular carcinoma cell line (HepG2) induced by boric acid at half mazimal inhibitory concentration (IC50). Tombuloğlu, Ayşegül; Çöpoğlu, Hülya; Güray, Nülüfer Tülün; Aydın Son, Yeşim (null; 2017-06-30) Boron is an essential micronutrient for many biological processes in plants and animals. In humans, adverse health outcomes are reported for both boron deprivation and excessive boron intake. Humans might get exposed to high doses of boron by consuming boron containing products or by working at boron enriched workplaces. In this study, the aim is to find out about the cytotoxicity mechanisms of boric acid, the most abundant form of boron containing compounds in human blood, on human hepatocellular carcinoma...

Citation Formats

A. ALTAY, D. İrtem Kartal, G. Sagdicoglu-Celep, N. T. Güray, and F. Bozoglu, “MODULATION OF CYTOCHROME P450s AND ANTIOXIDANT ENZYMES BY CAFFEIC ACID, p-COUMARIC ACID AND QUERCETIN-RICH ANATOLIAN PROPOLIS IN HUMAN ADENOCARCINOMA HT-29 CELLS,” FRESENIUS ENVIRONMENTAL BULLETIN, pp. 336–345, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/53313.