Hydrogen production by different strains of Rhodobacter sphaeroides

Gündüz, Ufuk
Yucel, M
Turker, L
Eroglu, L
Utilisation of solar energy by photosynthetic microorganisms for H-2 production attracts much interest due to unlimited supply of energy. It is important to identify the most effective strain in terms of hydrogen production for the feasibility of the process. Four different strains of Rhodobacter sp. were grown in a water-jacketed cylindrical glass-column photobioreactor under anaerobic conditions. Growth characteristics and hydrogen production rates were determined. Comparison between strains of Rhodobacter sp. was based on hydrogen production rate, its duration and the total volume of gas. The best strain was found to be R. sphaeroides L with a gas production rate of 0.0042 L-H2/h/L-culture and total gas volume of 328 ml in about 90 hours. No H2 gas production was observed for R. sphaeroides ATCC 17023 and R. capsulatus ATCC 23782 under the given experimental conditions. Presence of hydrogenase enzyme was also tested in all strains and relative hydrogenase activities were determined.


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Hydrogen production by solar thermal water splitting is an eco-friendly way of storing solar energy in chemical bonds. The most important obstacles for the viability and the commercialization of this technology are lower energy efficiencies and higher production costs compared to conventional hydrogen production ways such as steam reforming, coal gasification, and electrolysis of water. Two-step thermochemical hydrogen production by using solar energy is an alternative method to conventional hydrogen produc...
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Hydrogen is a clean energy alternative to fossil fuels. Photosynthetic bacteria produce hydrogen from organic compounds under anaerobic, nitrogen-limiting conditions through a light-dependent electron transfer process. In this study, the hydrogen production efficiency of phototrophic bacteria, Rhodobacter capsulatus and its Hup mutant strain (an uptake hydrogenase deleted strain) were tested on different initial acetate concentrations at fluctuating temperatures with indoor and outdoor photobioreactors. Ace...
Citation Formats
U. Gündüz, M. Yucel, L. Turker, and L. Eroglu, “Hydrogen production by different strains of Rhodobacter sphaeroides,” 2000, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/53467.