Production, purification and characterization of chitosanase from Penicillium spinulosum

Date

1998-11-01

Author

Ak, O
Bakir, U
Güray, Nülüfer Tülün

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A chitosan degrading fungus which had been isolated in our laboratory and identified as Penicillium spinulosum by the International Mycological Institute (England), was used for the production of chitosanase in a salt medium containing Rhizopus cell walls as the sole carbon source. Although chitosanase was produced under all the conditions tested about 1 % cell wall concentration maximized enzyme production. The enzyme was purified 50 fold by using ammonium sulfate precipitation and ion-exchange chromatography and then characterized. Optimal pH and temperature values of the enzyme were determined as pH 5 and 55 degrees C, respectively. The K-m and V-max values were found as 1.7 mg chitosan/ml and 146.4 mu mol glucosamine/ml-min, respectively by using Lineweaver-Burk plot. Approximately 15% and 50% activity losses were observed during a 20 minutes heat treatment at 45 and 65 degrees C at pH 4.2, respectively.

Subject Keywords

Biochemistry & molecular biology

URI

https://hdl.handle.net/11511/53719

Journal

BIOCHEMICAL ARCHIVES

Collections

Department of Biology, Article

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Citation Formats

O. Ak, U. Bakir, and N. T. Güray, “Production, purification and characterization of chitosanase from Penicillium spinulosum,” BIOCHEMICAL ARCHIVES, pp. 221–225, 1998, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/53719.