Dielectrophoretic detection of imatinib resistance in K562 Cells Using A lab-on-a-chip system

Demircan Yalçın, Yağmur
Toral, Taylan Berkın
Sukaş, Serkan
Yıldırım, Ender
Zorlu, Özge
Gündüz, Ufuk
Külah, Haluk
This study presents label-free detection of imatinib resistance in K562 cells via integrated dielectrophoretic detection and impedimetric counting on a lab-on-a-chip system. Two impedimetric counting units were placed upstream and downstream of dielectrophoretic detection unit to obtain differential cell count and consequently the trapping ratio. The trapping ratio up to 57% was achieved for imatinib resistant (~60-fold) K562 cells, while it was minimized to 20% for wild type K562 cells
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, 27 October 2019 through 31 October 2019


Assessment of effects of multi drug resistance on dielectric properties of K562 leukemic cells using electrorotation
Bahrieh, Garsha; Erdem, Murat; Ozgur, Ebru; Gündüz, Ufuk; Külah, Haluk (2014-01-01)
In this study, dielectric characterization of multidrug resistant (MDR) K562 human leukemia cells was carried out using a MEMS based electrorotation (ER) device with 3D electrodes. P-glycoprotein (P-gp) dependent MDR causes variation in cell dielectric properties (cell interior conductivity (sigma(i)), membrane capacitance (C-m) and total effective membrane conductance (G(m)*)) due to overexpression of P-gp, which modulates the activity of membrane-bound Cl- channels. Different cell populations resistant to...
Dielectric Characterization of Imatinib Resistant K562 Leukemia Cells through Electrorotation with 3-D Electrodes
Bahrieh, G.; Koydemir, H. Ceylan; Erdem, Murat; Ozgur, E.; Gündüz, Ufuk; Külah, Haluk (2013-11-06)
This study reports the use of electrorotation (ER) technique within a micro-fabricated device with 3D electrodes for dielectric characterization of Imatinib-resistant K562 (K562/IMA-0.2) human leukemic cells. The ER devices with 3D quadruple electrodes (30 mu m in height) were used in order to eliminate the fringing field effect on the rotation of cells. To induce the rotational moment on the cells, signals in phase quadrature were applied to the triangular electrodes. The rotation of cells measured at the ...
Demircan, Y.; Orguc, S.; Musayev, J.; Ozgur, E.; Erdem, Murat; Gündüz, Ufuk; Eminoglu, S.; Külah, Haluk; Akın, Tayfun (2015-06-25)
This paper presents a fully-integrated lab-on-a-chip (LOC) system for label-free detection and real-time counting of dielectrophoretically trapped multidrug resistant (MDR) K562 cells. The system integrates a parylene-based microfluidic DEP channel on top of a CMOS image sensor for the first time in the literature. The DEP channel can trap MDR K562 cells with 9 V-pp and 10 mu l/min flow rate, and the CMOS image sensor can detect the trapped cells as small as 3 mu m in diameter with a noise level of 28.3 e(-...
Aslan, Mahmut Kamil; Külah, Haluk (2017-06-22)
This paper presents a portable system integrating microfluidic dielectrophoresis (DEP) channel with a CMOS imager for label and lens free detection, and real-time counting of MCF-7 breast cancer cells. The DEP device is designed for trapping the MCF-7 that can simultaneously be imaged using a CMOS sensor. An android application is employed in order to record raw CMOS images. Cells are detected and counted using inbuilt image processing operations of the same application. Average counting accuracy of the sys...
Dielectrophoretic Separation of K562 Human Leukemia Cells From Leukocytes by Isolated 3D Electrodes
Bahrieh, Garsha; Özgür, Ebru; Külah, Haluk (null; 2014-09-03)
This paper presents the design, simulation, and fabrication of a MEMS based dielectrophoresis (DEP) platform for the separation and detection of the human leukemic cancer cells (K562) and its multidrug resistant (MDR) counterpart (K56/IMA-0.2 µM) from the human leukocyte mixture. The pro-posed DEP device, benefits from the 3D isolated electrodes inside a microfluidic channel. 3D elec-trodes (extruded 30 µm in z-direction) were isolated from the medium with thin parylene coating (~ 0.3 µm). In addition, the ...
Citation Formats
Y. Demircan Yalçın et al., “Dielectrophoretic detection of imatinib resistance in K562 Cells Using A lab-on-a-chip system,” Basel; Switzerland, 2019, p. 1358, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/87148.