Dielectrophoretic detection of imatinib resistance in K562 Cells Using A lab-on-a-chip system

2019-10-27
Demircan Yalçın, Yağmur
Toral, Taylan Berkın
Sukaş, Serkan
Yıldırım, Ender
Zorlu, Özge
Gündüz, Ufuk
Külah, Haluk
This study presents label-free detection of imatinib resistance in K562 cells via integrated dielectrophoretic detection and impedimetric counting on a lab-on-a-chip system. Two impedimetric counting units were placed upstream and downstream of dielectrophoretic detection unit to obtain differential cell count and consequently the trapping ratio. The trapping ratio up to 57% was achieved for imatinib resistant (~60-fold) K562 cells, while it was minimized to 20% for wild type K562 cells
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, 27 October 2019 through 31 October 2019

Suggestions

Dielectric Characterization of Imatinib Resistant K562 Leukemia Cells through Electrorotation with 3-D Electrodes
Bahrieh, G.; Koydemir, H. Ceylan; Erdem, Murat; Ozgur, E.; Gündüz, Ufuk; Külah, Haluk (2013-11-06)
This study reports the use of electrorotation (ER) technique within a micro-fabricated device with 3D electrodes for dielectric characterization of Imatinib-resistant K562 (K562/IMA-0.2) human leukemic cells. The ER devices with 3D quadruple electrodes (30 mu m in height) were used in order to eliminate the fringing field effect on the rotation of cells. To induce the rotational moment on the cells, signals in phase quadrature were applied to the triangular electrodes. The rotation of cells measured at the ...
Dielectrophoretic Separation of K562 Human Leukemia Cells From Leukocytes by Isolated 3D Electrodes
Bahrieh, Garsha; Özgür, Ebru; Külah, Haluk (null; 2014-09-03)
This paper presents the design, simulation, and fabrication of a MEMS based dielectrophoresis (DEP) platform for the separation and detection of the human leukemic cancer cells (K562) and its multidrug resistant (MDR) counterpart (K56/IMA-0.2 µM) from the human leukocyte mixture. The pro-posed DEP device, benefits from the 3D isolated electrodes inside a microfluidic channel. 3D elec-trodes (extruded 30 µm in z-direction) were isolated from the medium with thin parylene coating (~ 0.3 µm). In addition, the ...
NOS knockout or inhibition but not disrupting PSD-95-NOS interaction protect against ischemic brain damage
Kleinschnitz, Christoph; Mencl, Stine; Kleikers, Pamela W. M.; Schuhmann, Michael K.; Lopez, Manuela G.; Casas, Ana I.; Sürün, Bilge; Reif, Andreas; Schmidt, Harald H. H. W. (SAGE Publications, 2016-09-01)
Promising results have been reported in preclinical stroke target validation for pharmacological principles that disrupt the N-methyl-D-aspartate receptor-post-synaptic density protein-95-neuronal nitric oxide synthase complex. However, post-synaptic density protein-95 is also coupled to potentially neuroprotective mechanisms. As post-synaptic density protein-95 inhibitors may interfere with potentially neuroprotective mechanisms and sufficient validation has often been an issue in translating basic stroke ...
Dielectric Analysis of Changes in Electric Properties of Doxorubicin Resistant K562 Leukemic Cells Through Electrorotation with 3 D Electrodes
Garsha, Bahrieh; Erdem, Murat; Özgür, Ebru; Gündüz, Ufuk; Külah, Haluk (2013-10-31)
In this study, dielectric characterization of multidrug resistant (MDR) K562 human leukemia cells was carried out using a MEMS-based electrorotation (ER) device with 3D electrodes. Different cell populations were utilized, which were resistant to 0.1, 0.3, and 0.5 μM doxorubicin. The ER devices with 3D quadruple electrodes (30 urn in height) were used, in order to eliminate the fringing field effect on the rotation of cells. Signals in phase quadrature were applied to the polynomial electrodes, to induce th...
Assessment of effects of multi drug resistance on dielectric properties of K562 leukemic cells using electrorotation
Bahrieh, Garsha; Erdem, Murat; Ozgur, Ebru; Gündüz, Ufuk; Külah, Haluk (2014-01-01)
In this study, dielectric characterization of multidrug resistant (MDR) K562 human leukemia cells was carried out using a MEMS based electrorotation (ER) device with 3D electrodes. P-glycoprotein (P-gp) dependent MDR causes variation in cell dielectric properties (cell interior conductivity (sigma(i)), membrane capacitance (C-m) and total effective membrane conductance (G(m)*)) due to overexpression of P-gp, which modulates the activity of membrane-bound Cl- channels. Different cell populations resistant to...
Citation Formats
Y. Demircan Yalçın et al., “Dielectrophoretic detection of imatinib resistance in K562 Cells Using A lab-on-a-chip system,” Basel; Switzerland, 2019, p. 1358, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/87148.