Determination of Selected Endocrine Disrupter Compounds at Trace Levels in Sewage Sludge Samples

Date

2012-09-01

Author

Sonmez, Munire Selcen
Muz, Melis
Komesli, Okan Tarik
BAKIRDERE, Sezgin
Gökçay, Celal Ferdi

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

170
views

0
downloads

Simultaneous determination of endocrine disrupter compounds (EDCs), namely diltiazem, progesterone, benzylbutylphthalate (BBP), estrone, and carbamazepine (Cbz) were performed by using high performance LC-electrospray tandem MS. The ultrasound-aided sequential extraction of sludge samples was optimized to increase extraction efficiencies of the analytes; ranging between 93.0-98.3% recovery. The limit of detection values for diltiazem, progesterone, BBP, estrone, and Cbz were found as 0.78, 0.72, 0.24, 0.75, and 0.72 mu g/kg, respectively. Sludge samples taken from Ankara Tatlar; Hurma, Lara and Kemer of Antalya, and Middle East Technical University-vacuum rotating membrane wastewater treatment plant (WWTP) aeration tanks were analyzed for their EDCs contents under optimized conditions. Diltiazem was found in all the samples in the range between 116.4-180.8 ng/g while progesterone and estrone were not detected in any of the samples analyzed with the exception of Tatlar WWTP. The BBP concentration was between beyond detection and 9195.5 ng/g. In addition, Cbz was found in all the samples ranging from 25.6 to 118.8 ng/g.

Subject Keywords

Extraction efficiency, Health risk, Xenobiotic pollutant, Wastewater treatment, Ultrasound-aided sequential extraction

URI

https://hdl.handle.net/11511/57554

Journal

CLEAN-SOIL AIR WATER

DOI

https://doi.org/10.1002/clen.201100403

Collections

Department of Environmental Engineering, Article

Suggestions

OpenMETU
Core

Determination of selected natural hormones and endocrine disrupting compounds in domestic wastewater treatment plants by liquid chromatography electrospray ionization tandem mass spectrometry after solid phase extraction Muz, Melis; SÖNMEZ, MARGARET JEANNE M.; Komesli, Okan Tarik; Bakirdere, Sezgin; Gökçay, Celal Ferdi (2012-01-01) A new analytical method for the simultaneous determination of two natural hormones (progesterone and estrone) and two selected endocrine disrupter compounds (EDCs) (diltiazem and carbamazepine (Cbz)) was developed by liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) after pre-concentration with solid phase extraction (SPE). Influent and effluent samples taken from five different wastewater treatment plants throughout Turkey namely Hurma/Antalya, Lara/Antalya, Kemer-1 and ...
Usage of microwave and ultrasound in the extraction of essential oils and phenolic compounds İnce, Alev Emine; Şahin, Serpil; Şümnü, Servet Gülüm; Department of Food Engineering (2011) The objective of this study is to extract phenolic compounds from nettle and melissa by using microwave and ultrasound and to compare these methods with conventional extraction and maceration, respectively. Extraction of melissa essential oil was also studied. In extraction of phenolics, effects of extraction time (5-20 min for microwave; 5-30 min for ultrasound) and solid to solvent ratio (1:10, 1:20, 1:30 g/ml) on total phenolic content (TPC) were investigated for microwave and ultrasound extractions. Dif...
Simultaneous determination of selected endocrine disrupter compounds in wastewater samples in ultra trace levels using HPLC-ES-MS/MS Komesli, Okan Tarik; Bakirdere, Sezgin; Bayoren, Ceren; Gökçay, Celal Ferdi (2012-08-01) An analytical procedure for the simultaneous determination of six selected endocrine disrupter compounds (EDCs: diltiazem, progesterone, benzyl butyl phthalate (BBP), estrone, carbamazepine (Cbz), acetaminophen) was developed by liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ES-MS/MS). All of the parameters for HPLC and ES-MS/MS systems including mobile phase composition, flow rate, and sample injection volume were optimized to obtain not only the best separation of species int...
Determination of binary pesticide mixtures by an acetylcholinesterase-choline oxidase biosensor Kok, FN; Hasırcı, Vasıf Nejat (2004-02-15) In this study, acetylcholinesterase (AChE) and choline oxidase (ChO) were co-immobilized on poly (2-hydroxyethyl methacrylate) (pHEMA) membranes to construct a biosensor for the detection of anti-cholinesterase compounds. pHEMA membranes were prepared with the addition of SnCl4 to achieve the desired porosity. Immobilization of the enzymes was done by surface attachment via epichlorohydrin (Epi) and Cibacron Blue F3G-A (CB) activation. Enzyme immobilized membrane was used in the detection of anti-cholineste...
Determination of fumonisins B-1 and b2 in corn by liquid chromatography/mass spectrometry with immunoaffinity column cleanup: Single-laboratory method validation Senyuva, Hamide Z.; Özcan Kabasakal, Süreyya; Cimen, Dilek; Gilbert, John (2008-05-01) A single-laboratory method validation was conducted to establish the effectiveness of an immunoaffinity column cleanup procedure followed by liquid chromatography/mass spectrometry (LC/MS) for the determination of fumonisins B-1 and B-2 (FB1 + FB2) in corn. The test portion is extracted with acetonitrile-methanol-water (25 + 25 + 50). The extract is filtered, diluted with phosphate-buffered saline solution, and applied to an immunoaffinity column. FB1 + FB2 are removed with methanol and directly determined ...

Citation Formats

M. S. Sonmez, M. Muz, O. T. Komesli, S. BAKIRDERE, and C. F. Gökçay, “Determination of Selected Endocrine Disrupter Compounds at Trace Levels in Sewage Sludge Samples,” CLEAN-SOIL AIR WATER, pp. 980–985, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/57554.