Isolation of a chitosan degrading fungus, Penicillium spinulosum, and chitosanase production by the isolate

Date

1996-12-18

Author

Ak, O
Bakir, U
Güray, Nülüfer Tülün

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

141
views

0
downloads

A chitosan degrading fungus was isolated from chitosan and then identified as Penicillium spinulosum by the International Mycological Institute, England. The isolate was used for the production of chitosanase in a salts medium with either chitosan or Rhizopus cell walls as the sole carbon source. Chitosanase was produced under all the conditions tested, however better yields were obtained by using Rhizopus cell walls.

URI

https://hdl.handle.net/11511/55409

Collections

Department of Biology, Conference / Seminar

Suggestions

OpenMETU
Core

Production, purification and characterization of chitosanase from Penicillium spinulosum Ak, O; Bakir, U; Güray, Nülüfer Tülün (1998-11-01) A chitosan degrading fungus which had been isolated in our laboratory and identified as Penicillium spinulosum by the International Mycological Institute (England), was used for the production of chitosanase in a salt medium containing Rhizopus cell walls as the sole carbon source. Although chitosanase was produced under all the conditions tested about 1 % cell wall concentration maximized enzyme production. The enzyme was purified 50 fold by using ammonium sulfate precipitation and ion-exchange chromatogra...
Purification, characterization, and identification of a novel bifunctional catalase-phenol oxidase from Scytalidium thermophilum Kocabas, Didem Sutay; Bakir, Ufuk; Phillips, Simon E. V.; McPherson, Michael J.; Ögel, Zümrüt Begüm (Springer Science and Business Media LLC, 2008-06-01) A novel bifunctional catalase with an additional phenol oxidase activity was isolated from a thermophilic fungus, Scytalidium thermophilum. This extracellular enzyme was purified ca. 10-fold with 46% yield and was biochemically characterized. The enzyme contains heme and has a molecular weight of 320 kDa with four 80 kDa subunits and an isoelectric point of 5.0. Catalase and phenol oxidase activities were most stable at pH 7.0. The activation energies of catalase and phenol oxidase activities of the enzyme ...
ANALYSIS OF PLASMIDS MEDIATING ANT (2'') AND AAC(3)-II BASED GENTAMICIN RESISTANCE IN CLINICAL ISOLATES OF ENTEROBACTERIACEAE Kocabıyık, Semra (Elsevier BV, 1989-07-01) The genetics of ANT(2″) and AAC(3)-II mediated gentamicin resistance in Gram-negative bacteria obtained from Hacettepe University Hospital in Turkey, was studied. The plasmid DNA analyzed by agarose gel electrophoresis revealed a heterogeneous plasmid population. Conjugal transfer appeared to be an efficient mechanism for the dissemination of resistance to gentamicin/tobramycin/kanamycin/streptomycin/chloramphenicol/cephalothin, which was the common resistance pattern in the population. Plasmids of 46, 60, ...
Interpretation of SARS-CoV-2 behaviour on different substrates and denaturation of virions using ethanol: an atomic force microscopy study ÇELİK, ÜMİT; ÇELİK, KÜBRA; Celik, Suleyman; ABAYLI, HASAN; ŞAHNA, KEZBAN; TONBAK, ŞÜKRÜ; AŞÇI TORAMAN, ZÜLAL; Oral, Ahmet (Royal Society of Chemistry (RSC), 2020-12-01) Coronavirus (SARS-CoV-2) is a respiratory infection virus that was first detected in Wuhan, China. The virus causes COVID-19 disease and the outbreak was recognised as a pandemic by the World Health Organization (WHO) in March 2020. SARS-CoV-2 virion was first imaged using cryo-electron microscopy by the Chinese Center for Disease Control and Prevention (CDC). Atomic Force Microscopy is a unique technique that can allow imaging of biomolecules under different conditions. In this work, we used Atomic Force M...
Isolation and characterisation of antioxidant compounds in yellow rose root extract Kyejussa, Yusuf; Çoruh, Nursen; Department of Chemistry (2009) A phytochemical investigation on methanolic extract of roots of yellow rose led to the isolation of a catechin gallate. The crude extract first underwent fractionation using petroleum ether, chloroform, ethylacetate, butanol with water as solvents in their respective order. The emerging solvent fractions were subjected to further separation using lipophilic sephadex (LH-20), and silica gel column chromatography to isolate pure compounds. Analytical thin layer chromatography (TLC) was used to confirm the pre...

Citation Formats

O. Ak, U. Bakir, and N. T. Güray, “Isolation of a chitosan degrading fungus, Penicillium spinulosum, and chitosanase production by the isolate,” 1996, vol. 53, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/55409.