In Turkish wheat cultivars the resistance allele of LR34 is ineffective against leaf rust

Yildirim, Kubilay
Boylu, Baris
Atici, Elif
Kahraman, Turhan
Akkaya, Mahinur
Rust and powdery mildew diseases of wheat are among the oldest and most harmful diseases confining the yield of wheat worldwide. Development of resistant wheat cultivars with durable or race-nonspecific resistance genes is the main objective for many breeding programs. One of these genes, Lr34/Yr18/Pm38, was found to confer partial and durable resistance against rust pathogens as well as powdery mildew. This multiple disease resistant locus was recently found to comprise a single gene, which encodes an adenosine triphosphate-binding cassette transporter (ABC-transporter) of the pleiotropic drug resistance (PDR) subfamily. Differences in the alleles of resistant and susceptible cultivars enabled to develop six allele-specific markers. In this study, 46 different Turkish bread wheat cultivars were screened using these specific markers, which indicated that 13 cultivars carry the resistance allele of that gene. To compare this molecular finding with phenotypic observations, a field trial with artificial inoculation of Puccinia recondita was performed. Interestingly, all cultivars having the resistance allele were found to be susceptible. Although Lr34 has mediated resistance against leaf rust for over 40 years and has been used globally as an effective component of rust resistance in many breeding programs, this adult plant resistance is ineffective in the studied Turkish bread wheat cultivars.


Genetic screening of Turkish wheat varieties for the durable resistance gene, Lr34
Boylu, Barış; Akkaya, Mahinur S.; Hakkı, Erdoğan Eşref; Department of Biotechnology (2011)
Wheat diseases such as rusts and powdery mildews are among the most important and ancient diseases that affect wheat cultivation worldwide. The pathogen race specific resistance genes cannot maintain long lasting resistance. On the other hand, the presence of genes confers the non-race specific resistance last much longer. The durable resistance phenotypes in wheat against various rust and powdery mildew diseases were reported as Lr34, Yr18, and Pm38 separately; nevertheless, they were known to locate very ...
Bacterial expression of an effector protein of yellow rust pathogen and a resistance protein of wheat and characterization of the effector protein
Erdoğan, Sayıt Mahmut; Akkaya, Mahinur S.; Özçubukçu, Salih; Department of Biotechnology (2019)
Yellow rust is one of the most important wheat disease encountered in large parts of the world and in our country caused by a pathogen which is called as Puccinia striiformis f. sp. tritici (Pst). Rapid alterations in the pathogen virulency can make previously resistant varieties susceptible to the disease. Finding out common or species-specific genes, participated in the plant-pathogen interactions will provide an understanding of the biological mechanisms of the disease. Within the scope of this thesis fo...
In-depth secretome analysis of Puccinia striiformis f. sp. tritici in infected wheat uncovers effector functions
Ozketen, Ahmet Caglar; Andaç Özketen, Ayşe; Dagvadorj, Bayantes; Demiralay, Burak; Akkaya, Mahinur (2020-12-01)
The importance of wheat yellow rust disease, caused by Puccinia striiformis f. sp. tritici (Pst), has increased substantially due to the emergence of aggressive new Pst races in the last couple of decades. In an era of escalating human populations and climate change, it is vital to understand the infection mechanism of Pst in order to develop better strategies to combat wheat yellow disease. The present study focuses on the identification of small secreted proteins (SSPs) and candidate-secreted effector pro...
Characterization of yellow rust and stem rust resistant and sensitive durum wheat lines at molecular level by using biophysical methods
Kansu, Çiğdem; Kaya, Zeki; Severcan, Feride; Department of Biology (2011)
Stem rust and Yellow rust diseases are the two major wheat fungal diseases causing considerable yield losses in Turkey and all around the world. There are studies which are carried out to identify and utilize resistance sources in order to obtain resistant lines of wheat. However, virulent pathotypes are continuously being important threats to wheat production and yield. For that reason, new approaches for rapid identification are needed. The aim of this study was to investigate and to understand the struct...
Genetic diversity of scald (rhynchosporium secalis) disease resistant and sensitive Turkish barley seed sources as determined with simple sequence repeats
Dizkırıcı, Ayten; Kaya, Zeki; Department of Biology (2006)
Scald disease (Rhynchosporium secalis) is one of the major plant diseases causing considerable yield loss in barley (Hordeum vulgare) plantations in Turkey. To develop, scald resistant barley varieties, C.R.I.F.C. of Turkey has a large accumulated collection of barley seed sources in hand, but these samples are difficult to be followed and used in the breeding programs due to lack of genetic studies on them. Thus, the objective of this study was to characterize and fingerprint of eighty barley seed sources,...
Citation Formats
K. Yildirim, B. Boylu, E. Atici, T. Kahraman, and M. Akkaya, “In Turkish wheat cultivars the resistance allele of LR34 is ineffective against leaf rust,” JOURNAL OF PLANT DISEASES AND PROTECTION, pp. 135–141, 2012, Accessed: 00, 2020. [Online]. Available: