Effect of progesterone on DPPC membrane: Evidence for lateral phase separation and inverse action in lipid dynamics

Date

2005-08-15

Author

Korkmaz, F
Severcan, Feride

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Interactions of progesterone with zwitterionic dipalmitoyl phosphatidylcholine (DPPC) triultilamellar liposomes were investigated as a function of temperature and progesterone concentration by using three non-invasive techniques namely Fourier transform infrared spectroscopy, turbidity at 440 nm, and differential scanning calorimetry. The results reveal that progesterone changes the physical properties of DPPC bilayers by decreasing the main phase-transition temperature, abolishing the pre-transition, broadening the phase-transition profile, disordering the system both in gel and liquid crystalline phase, increasing the dynamics at low concentrations whereas stabilizing the membrane at high concentrations, and inducing phase separation. Progesterone does not change the hydration of the C=O groups, while it strengthens the hydrogen bonding between the PO (2) over bar groups of lipids and the water molecules around. (c) 2005 Elsevier Inc. All rights reserved.

Subject Keywords

Biophysics, Biochemistry, Molecular Biology

URI

https://hdl.handle.net/11511/57281

Journal

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

DOI

https://doi.org/10.1016/j.abb.2005.06.013

Collections

Department of Biology, Article

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Citation Formats

F. Korkmaz and F. Severcan, “Effect of progesterone on DPPC membrane: Evidence for lateral phase separation and inverse action in lipid dynamics,” ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, pp. 141–147, 2005, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/57281.