Genotyping of Anatolian doubled-haploid durum lines with SSR markers

Hakki, EE
Savaskan, C
Akkaya, Mahinur
In this study, doubled haploid lines generated from the durum wheat varieties, selections from Middle Anatolian landraces, 'Cakmak-79', 'Berkmen-469' and 'Kunduru-1149' (Savaskan et al., 1997), were analyzed using ten highly polymorphic microsatellite markers for genotyping and evaluation of genetic relationships between and within the doubled-haploid (DH) lines. The average PIC value was found to be 0.531. Populations of doubled-haploid lines of landrace selected cultivars 'Cakmak-79' and 'Kunduru-1149', were the two most distant populations with (delta mu)(2) = 1.42. 'Berkmen-469' x 'Cakmak-79' and 'Berkmen-469' x 'Kunduru-1149' yielded similar genetic distances, (delta mu)(2) of 0.84 and 0.85, respectively. In addition, the genetic relationship between the progenitors of the DH lines together with other durum wheat varieties was analyzed. A meaningful relationship was obtained based on available pedigree information on the cultivars.


SSR markers in the genetic relationship studies of Turkish durum wheat varieties
Akkaya, Mahinur; Dograr, N; Incirli, A; Hakki, EE; Buyukunal, EB; Bilgic, H (2000-06-09)
DNA markers are the essential tools for differentiating genotypic characters of varieties and cultivars. Microsatellite or Simple Sequence Repeat (SSR) markers are PCR-based molecular markers. SSRs are the type of markers that can be used most efficiently for DNA fingerprinting due to their reproducible and high level of polymorphic features. The variations in the number of nucleotide repeats, SSR length polymorphism, were detected by separating (alpha P-32)-dATP labeled PCR products obtained with locus spe...
Genetic differentiation of Abies equi-trojani (Asch. & Sint. ex Boiss) Mattf. populations from Kazdaǧι, Turkey and the genetic relationship between Turkish firs belonging to the Abies nordmanniana Spach complex
Kaya, Zeki; Neale, David Brian (2008-02-05)
The present study aimed to test the utility of RAPD (randomly amplified polymorphic DNA) and cpSSR (simple sequence repeats) markers for in situ gene conservation programs for fir species, as well as for determining the genetic similarities between the Abies nordmanniana Spach species complex (A. nordmanniana, A. bornmuelleriana Matff., A. equi-trojani (Asch. & Sint. ex Boiss) Mattf.) and between populations of A. equi-trojani, which is a narrow-endemic to Turkey. For this purpose, DNA was extracted and poo...
Genetic structure of black pine (Pinus nigra ARNOLD subspecies pallasiana) populations sampled from the Bolkar Mountains
Tolun, AA; Velioğlu, E; Çengel, Burcu N; Kaya, Zeki (2000-01-01)
In order to determine the magnitude and pattern of genetic diversity among Anatolian Black pine (Pinus nigra ARNOLD subspecies pallasiana) populations sampled in Bolkar Mountains and to recommend the potential populations which may be suitable for in situ conservation of genetic resources in this species, isoenzymes from 14 enzyme systems were investigated by starch gel electrophoresis. For this reason, open pollinated seed megagametophytes of half-sib families originated from the four populations (Camliyay...
Genetic and physiology of cold and drought resistance in Turkish red pine (Pinus brutia, Ten.) populations from southern Turkey
Kandemir, Gaye Eren; Kaya, Zeki; Önde, Sertaç; Department of Biology (2002)
The purpose of the study was to determine the pattern of genetic variation in morphological and physiological traits associated with cold and drought stress using seedling from over-exploited and natural populations of Pinus brutia Ten. (Turkish red pine) from southern Turkey. To determine the pattern of genetic variation existing in over-exploited and natural populations, seedlings from 6 populations each with 40 families were raised in Ankara forest nursery from 1998-2000. Three of the populations (Yaylaa...
Discriminating durum wheat cultivars using highly polymorphic simple sequence repeat DNA markers
Dograr, N; Akin-Yalin, S; Akkaya, Mahinur (2000-08-01)
The winter type durum wheat varieties of Anatolia used in this study were differentiated for the first time by using simple sequence repeat (SSR) DNA markers or microsatellites. Seven microsatellite markers were used to distinguish four well-adapted landrace selections. five cultivars and seven recently obtained advancing lines. The loci of seven microsatellites were all homozygous, but the WMS6 locus occurred with two alleles in all the genotypes. The genotypes were all distinguished from each other, with ...
Citation Formats
E. Hakki, C. Savaskan, and M. Akkaya, “Genotyping of Anatolian doubled-haploid durum lines with SSR markers,” EUPHYTICA, pp. 257–262, 2001, Accessed: 00, 2020. [Online]. Available: