Discriminating durum wheat cultivars using highly polymorphic simple sequence repeat DNA markers

Date

2000-08-01

Author

Dograr, N
Akin-Yalin, S
Akkaya, Mahinur

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

168
views

0
downloads

The winter type durum wheat varieties of Anatolia used in this study were differentiated for the first time by using simple sequence repeat (SSR) DNA markers or microsatellites. Seven microsatellite markers were used to distinguish four well-adapted landrace selections. five cultivars and seven recently obtained advancing lines. The loci of seven microsatellites were all homozygous, but the WMS6 locus occurred with two alleles in all the genotypes. The genotypes were all distinguished from each other, with the number of alleles ranging from live to 13. The lowest and highest polymorphism information content (PIC) values were observed to be 0.609 and 0.872, respectively. Three markers alone, WMS6, WMS30 and WMS120. can distinguish all 16 genotypes. UPGMA dendogram, based on a similarity matrix by a simple matching coefficient algorithm, is in accordance with the available pedigree information.

Subject Keywords

Triticum durum, DNA fingerprinting, Differentiation, Microsatellites, SSR

URI

https://hdl.handle.net/11511/57760

Journal

PLANT BREEDING

DOI

https://doi.org/10.1046/j.1439-0523.2000.00497.x

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Assessment of genetic relationships in durum wheat cultivars using AFLP markers Incirli, A; Akkaya, Mahinur (2001-06-01) The genetic relationships were assessed for the first time in Turkish durum wheat cultivars using AFLP markers. In the analysis, 18 AFLP primer combinations resulted in a total of 189 polymorphic loci. All of the selective primers used are Eco and Mse primers with three nucleotide extentions on the 3' ends. The number of polymorphic markers per primer combination ranged from 4 to 24. The relationships, among nine winter and six spring type durum wheat cultivars, obtained with various algorithms are in accor...
Genotyping of Anatolian doubled-haploid durum lines with SSR markers Hakki, EE; Savaskan, C; Akkaya, Mahinur (2001-01-01) In this study, doubled haploid lines generated from the durum wheat varieties, selections from Middle Anatolian landraces, 'Cakmak-79', 'Berkmen-469' and 'Kunduru-1149' (Savaskan et al., 1997), were analyzed using ten highly polymorphic microsatellite markers for genotyping and evaluation of genetic relationships between and within the doubled-haploid (DH) lines. The average PIC value was found to be 0.531. Populations of doubled-haploid lines of landrace selected cultivars 'Cakmak-79' and 'Kunduru-1149', w...
SSR markers in the genetic relationship studies of Turkish durum wheat varieties Akkaya, Mahinur; Dograr, N; Incirli, A; Hakki, EE; Buyukunal, EB; Bilgic, H (2000-06-09) DNA markers are the essential tools for differentiating genotypic characters of varieties and cultivars. Microsatellite or Simple Sequence Repeat (SSR) markers are PCR-based molecular markers. SSRs are the type of markers that can be used most efficiently for DNA fingerprinting due to their reproducible and high level of polymorphic features. The variations in the number of nucleotide repeats, SSR length polymorphism, were detected by separating (alpha P-32)-dATP labeled PCR products obtained with locus spe...
Identification and Characterization of 33 Bacillus cereus sensu lato Isolates from Agricultural Fields from Eleven Widely Distributed Countries by Whole Genome Sequencing Zervas, Athanasios; Aggerbeck, Marie Ronne; Allaga, Henrietta; GÜZEL, MUSTAFA; Hendriks, Marc; Jonuskiene, IIona; Kedves, Orsolya; Kupeli, Ayse; Lamovsek, Janja; Muelner, Pascal; Munday, Denise; Namlı, Şahin; Samut, Hilal; Tomicic, Ruzica; Tomicic, Zorica; Yeni, Filiz; Zghal, Raida Zribi; Zhao, Xingchen; Sanchis-Borja, Vincent; Hendriksen, Niels Bohse (2020-12-01) The phylogeny, identification, and characterization of 33 B. cereus sensu lato isolates originating from 17 agricultural soils from 11 countries were analyzed on the basis of whole genome sequencing. Phylogenetic analyses revealed all isolates are divided into six groups, which follows the generally accepted phylogenetic division of B. cereus sensu lato isolates. Four different identification methods resulted in a variation in the identity of the isolates, as none of the isolates were identified as the same...
Assessment of genetic variation of bread wheat varieties using microsatellite markers Akkaya, Mahinur; Buyukunal-Bal, EB (2004-01-01) The DNA fingerprinting profiles of 11 bread wheat cultivars, grown in Turkey, was obtained by using 19 highly polymorphic wheat microsatellites. Up to six randomly selected individuals from each of these cultivars were subjected to analysis. Estimated polymorphism information content (PIC) values among 65 individual genotypes for 19 loci were between 0.36 and 0.87 with an average value of 0.68. The numbers of observed alleles were between 2 and 9, with an average value of 5.42. These relationships were asse...

Citation Formats

N. Dograr, S. Akin-Yalin, and M. Akkaya, “Discriminating durum wheat cultivars using highly polymorphic simple sequence repeat DNA markers,” PLANT BREEDING, pp. 360–362, 2000, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/57760.