Detection and Enumeration of Salmonella in Biosolids by Loop-Mediated Isothermal Amplification

Date

2015-12-31

Author

Murdoch, Robert Waller

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Loop-mediated isothermal amplification (LAMP) is a relatively new technique in molecular biology that offers a rapid, inexpensive, resistant to inhibition, and highly-specific alternative to classical polymerase chain reaction (PCR). One of the main advantages of LAMP is that the amplification is isothermal, thus requiring only a single inexpensive heat block or water bath. Further, the high-specificity of LAMP greatly reduces the likelihood of false-positives, which PCR is very prone to. The polymerization enzyme used in LAMP is also highly resistant to conditions that would inhibit Taq polymerase, the classical PCR DNA polymerase enzyme. Due to these advantages, LAMP is increasingly being explored as a superior method for the detection and quantification of specific pathogens in food and water supplies. This study is aimed at adaptation and application of current novel molecular based technique to enumeration of Salmonella. By obtaining Salmonella counts by the best available culture-based technique in parallel, this project will offer reliable counts of Salmonella in Turkish biosolids in a manner that allows comparison to international numbers. The goal of this project is to apply a novel molecular biology procedure, loop-mediated isothermal amplification (LAMP), for the quantification of Salmonella in Turkish biosolids. The application of this procedure to Salmonella in sewage systems has not been reported in the literature.

Subject Keywords

Teknolojik Bilimler (Mühendislikler), Çevre Mühendisliği ve Teknolojisi, Çevre Biyolojisi, Biyoteknoloji, Mikrobiyoloji, Moleküler Biyoloji ve Genetik

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https://hdl.handle.net/11511/59368

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Department of Environmental Engineering, Project and Design

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Citation Formats

R. W. Murdoch, “Detection and Enumeration of Salmonella in Biosolids by Loop-Mediated Isothermal Amplification,” 2015. Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/59368.