Poly (I:C)- and doxorubicin-loaded magnetic dendrimeric nanoparticles affect the apoptosis-related gene expressions in MCF-7 cells

Download

10.3906_biy-1912-71.pdf

Date

2020-8-19

Author

Khodadust, Rouhollah
Alpsoy, Aktan
Ünsoy, Gözde
Gündüz, Ufuk

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

12
views

14
downloads

Use of nanoparticles as drug carrier vectors has great potential to circumvent the limitations associated with chemotherapy, including drug resistance and destructive side effects. For this purpose, magnetic generation 4 dendrimeric nanoparticles were prepared to carry chemotherapeutic agent doxorubicin (G 4-DOX) and immune modulator polyinosinic:polycytidylic acid [Poly(I:C)]. As previously reported, DOX and Poly(I:C) was loaded onto G 4 nanoparticles (PIC-G 4-DOX). Cellular internalization study using confocal microscopy demonstrated high levels of cellular internalization of PIC-G 4-DOX nanoparticles by MCF-7 cells. This resulted in higher efficacy of PIC-G 4-DOX nanoparticles in killing MCF-7 breast cancer cells. Alteration in the expression levels of selected genes was determined by RT-qPCR analyses. Proapoptotic NOXA, PUMA, and BAX genes were upregulated, and SURVIVIN, APOLLON, and BCL-2 genes were downregulated, indicating the cell-killing effectiveness of PIC-G 4-DOX nanoparticles. Gene expression analysis provided some insights into the possible molecular mechanisms on cytotoxicity of DOX and Poly(I:C) delivered through G 4 magnetic nanoparticles. The results demonstrated that PIC-G 4-DOX can be useful for targeted delivery affecting apoptotic pathways, resulting in an advanced degree of cancer-cell-killing. They are promising for targeting cancer-cells because of their stability, biocompatibility, higher internalization, and toxicity.

Subject Keywords

Targeted drug delivery, magnetic dendrimeric nanoparticles, Poly(I:C), doxorubicin, gene expression

URI

https://hdl.handle.net/11511/63108

Journal

TURKISH JOURNAL OF BIOLOGY

DOI

https://doi.org/10.3906/biy-1912-71

Collections

Department of Biology, Article

Citation Formats

R. Khodadust, A. Alpsoy, G. Ünsoy, and U. Gündüz, “Poly (I:C)- and doxorubicin-loaded magnetic dendrimeric nanoparticles affect the apoptosis-related gene expressions in MCF-7 cells,” TURKISH JOURNAL OF BIOLOGY, vol. 44, no. 4, pp. 133–144, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/63108.