Poly (I:C)- and doxorubicin-loaded magnetic dendrimeric nanoparticles affect the apoptosis-related gene expressions in MCF-7 cells

2020-8-19
Khodadust, Rouhollah
Alpsoy, Aktan
Ünsoy, Gözde
Gündüz, Ufuk
Show full item record
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
155
views
107
downloads
Use of nanoparticles as drug carrier vectors has great potential to circumvent the limitations associated with chemotherapy, including drug resistance and destructive side effects. For this purpose, magnetic generation 4 dendrimeric nanoparticles were prepared to carry chemotherapeutic agent doxorubicin (G 4-DOX) and immune modulator polyinosinic:polycytidylic acid [Poly(I:C)]. As previously reported, DOX and Poly(I:C) was loaded onto G 4 nanoparticles (PIC-G 4-DOX). Cellular internalization study using confocal microscopy demonstrated high levels of cellular internalization of PIC-G 4-DOX nanoparticles by MCF-7 cells. This resulted in higher efficacy of PIC-G 4-DOX nanoparticles in killing MCF-7 breast cancer cells. Alteration in the expression levels of selected genes was determined by RT-qPCR analyses. Proapoptotic NOXA, PUMA, and BAX genes were upregulated, and SURVIVIN, APOLLON, and BCL-2 genes were downregulated, indicating the cell-killing effectiveness of PIC-G 4-DOX nanoparticles. Gene expression analysis provided some insights into the possible molecular mechanisms on cytotoxicity of DOX and Poly(I:C) delivered through G 4 magnetic nanoparticles. The results demonstrated that PIC-G 4-DOX can be useful for targeted delivery affecting apoptotic pathways, resulting in an advanced degree of cancer-cell-killing. They are promising for targeting cancer-cells because of their stability, biocompatibility, higher internalization, and toxicity.
Targeted drug delivery, magnetic dendrimeric nanoparticles, Poly(I:C), doxorubicin, gene expression
https://hdl.handle.net/11511/63108
TURKISH JOURNAL OF BIOLOGY
Department of Biology, Article

Suggestions

Synthesis of Doxorubicin loaded magnetic chitosan nanoparticles for pH responsive targeted drug delivery
ÜNSOY, GÖZDE; Khodadust, Rouhollah; Yalcin, Serap; Mutlu, Pelin; Gündüz, Ufuk (2014-10-01)
Targeted drug delivery is a promising alternative to overcome the limitations of classical chemotherapy. In an ideal targeted drug delivery system carrier nanoparticles would be directed to the tumor tissue and selectively release therapeutic molecules. As a novel approach, chitosan coated magnetic nanoparticles (CS MNPs) maintain a pH dependent drug delivery which provides targeting of drugs to the tumor site under a magnetic field. Among various materials, chitosan has a great importance as a pH sensitive...
Chitosan magnetic nanoparticles for pH responsive Bortezomib release in cancer therapy
ÜNSOY, GÖZDE; Yalcin, Serap; Khodadust, Rouhollah; Mutlu, Pelin; Onguru, Onder; Gündüz, Ufuk (2014-06-01)
The use of nanotechnology in cancer treatment offers exciting opportunities, including the possibility of destroying tumors with minimal damage to healthy tissue by novel targeted drug delivery systems. pH differences between healthy and tumor microenvironment provide pH responsive release of drugs at tumor site via smart nanoparticles. In this study, chitosan coated superparamagnetic iron oxide nanoparticles (CS MNPs) were in situ synthesized by ionic crosslinking method as nanocarrier systems and loaded w...
Investigation of the Therapeutic Effects of Palbociclib Conjugated Magnetic Nanoparticles on Different Types of Breast Cancer Cell Lines
Parsian, Maryam; MUTLU, PELİN; Taghavi Pourianazar, Negar; Yalcin Azarkan, Serap; Gündüz, Ufuk (2023-01-01)
Introduction: Drug targeting and controlled drug release systems in cancer treatment have many advantages over conventional chemotherapy in terms of limiting systemic toxicity, side effects, and overcoming drug resistance. Methods and Results: In this paper, fabricating nanoscale delivery system composed of magnetic nanoparticles (MNPs) covered with poly-amidoamine (PAMAM) dendrimers and using its advantages were fully used to help the chemotherapeutic drug, Palbociclib, effectively reach tumors, specifical...
Synthesis and characterization of fatty acid based hyperbranched polymers for anti-cancer drug delivery
Güç, Esra; Gündüz, Ufuk; Department of Biology (2008)
Conventional methods of chemotherapy requires novel therapy systems due to serious side effects and inefficiency of drug administration. In recent years many studies are carried out to improve drug delivery systems. Polymers are one of the most important elements for drug delivery research due to their versatility. By the discovery of dendritic polymers, drug delivery studies gained a new vision. Highly branched monodisperse structure, multiple sites of attachment, well-defined size and controllable physica...
Smart Drug Delivery Systems in Cancer Therapy
ÜNSOY, GÖZDE; Gündüz, Ufuk (2018-01-01)
Background: Smart nanocarriers have been designed for tissue-specific targeted drug delivery, sustained or triggered drug release and co-delivery of synergistic drug combinations to develop safer and more efficient therapeutics.
Citation Formats
R. Khodadust, A. Alpsoy, G. Ünsoy, and U. Gündüz, “Poly (I:C)- and doxorubicin-loaded magnetic dendrimeric nanoparticles affect the apoptosis-related gene expressions in MCF-7 cells,” TURKISH JOURNAL OF BIOLOGY, pp. 133–144, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/63108.
METU IIS - Integrated Information Service open@metu.edu.tr