Synthesis of biomimetic Ca-hydroxyapatite powders at 37 degrees C in synthetic body fluids

Tas, AC
An important inorganic phase for synthetic bone applications, calcium hydroxyapatite (HA, Ca-10(PO4)(6)(OH)(2)), was prepared as a nano-sized (similar to 50 nm), homogeneous and high-purity ceramic powder from calcium nitrate tetrahydrate and diammonium hydrogen phosphate salts dissolved in modified synthetic body fluid (SBF) solutions at 37 degrees C and pH of 7.4 using a novel chemical precipitation technique. The synthesized precursors were found to easily reach a phase purity >99% after 6 h of calcination in air atmosphere at 900 degrees C, following oven drying at 80 degrees C. There was observed, surprisingly, no decomposition of HA into the undesired beta-TCP phase even after heating at 1600 degrees C in air for 6 h. This observation showed the superior high-temperature stability of such 'biomimetic' HA powders as compared to those reported in previous studies. The former powders were also found to contain trace amounts of Na and Mg ions, originating from the use of SBF solutions instead of pure water during their synthesis. Characterization and chemical analaysis of the synthesized powders were performed by X-ray powder diffraction, energy-dispersive X-ray spectroscopy, Fourier-transform infra-red spectroscopy, scanning electron microscopy, and inductively coupled plasma atomic emission spectroscopy. (C) 2000 Published by Elsevier Science Ltd. All rights reserved.


Preparation of porous Ca-10(PO4)(6)(OH)(2) and beta-Ca-3(PO4)(2) bioceramics
Engin, NO; Tas, AC (2000-07-01)
Submicrometer-sized, pure calcium hydroxyapatite (HA, (Ca-10(PO4)(6)(OH)(2))) and eta-tricalcium phosphate (beta-TCP, Ca-3(PO4)(2)) bioceramic powders, that have been synthesized via chemical precipitation techniques, were used in the preparation of aqueous slurries that contained methyl cellulose to manufacture porous (70%-95% porosity) HA or beta-TCP ceramics. The pore sizes in HA bioceramics of this study were 200-400 mu m, whereas those of beta-TCP bioceramics were 100-300 mu m. The pore morphology and ...
Fabrication and characterization of porous hydroxyapatite and biphasic calcium phosphate ceramic as bone substitutes
Koc, N; Timucin, M; Korkusuz, Feza (2004-01-01)
In the present study, the calcium phosphate powders (HAP, HAP-TCP) were produced by a precipitation technique involving the use of aqueous solutions of calcium nitrate and diammonium hydrogen phosphate. Preforms of calcium phosphate ceramics were produced by using a modified slip casting technique. Porosity in the cast and in the sintered ceramic was obtained through custom made PMMA beads similar to200-300 mum size. The green ceramic samples were prepared by suspending the apatite powder and PMMA beads in ...
Synthesis of calcium carbonate particles for biomedical applications
Oral, Çağatay Mert; Ercan, Batur; Department of Metallurgical and Materials Engineering (2020)
Calcium carbonate (CaCO3) particles have been widely used in biomedical applications owing to their biocompatibility and biodegradability. In order to effectively utilize CaCO3 particles in biomedical applications, their physical and chemical properties should be systematically controlled. However, this is a challenging task due to the presence of three different anhydrous CaCO3 polymorphs having complex crystallization behavior. In this thesis, CaCO3 particles were synthesized at distinct environments to c...
Preparation, modification, and characterization of acrylic cements
Basgorenay, B; Ulubayram, K; Serbetci, K; Onurhan, E; Hasırcı, Nesrin (2006-03-15)
Acrylic cements with different compositions were prepared by mixing the solid part (composed of poly(methyl methacrylate), PMMA, and benzoyl peroxide, BPO) and the liquid part (composed of methyl methacrylate, MMA, and N,N-dimethyl-p-toluidine, DMPT), modified by addition of hydroxyapatite (HA) and ammonium nitrate (AN) and characterized by measuring thermal and mechanical properties. Three sets of samples were prepared. For B-group, the total amount of solid including HA was constant but the PMMA to HA rat...
Synthesis and characterization of nanosized calcium phosphates by flame spray pyrolysis, and their effect on osteogenic differentiation of stem cells
Ataol, Sibel; Tezcaner, Ayşen; Duygulu, Ozgur; Keskin, Dilek; Machin, Nesrin E. (2015-02-14)
The present study evaluates the synthesis of biocompatible osteoconductive and osteoinductive nano calcium phosphate (CaP) particles by industrially applied, aerosol-derived flame spray pyrolysis method for biomedical field. Calcium phosphate nanoparticles were produced in a range of calcium-to-phosphorus ratio, (1.20-2.19) in order to analyze the morphology and crystallinity changes, and to test the bioactivity of particles. The characterization results confirmed that nanometer-sized, spherical calcium pho...
Citation Formats
A. Tas, “Synthesis of biomimetic Ca-hydroxyapatite powders at 37 degrees C in synthetic body fluids,” BIOMATERIALS, pp. 1429–1438, 2000, Accessed: 00, 2020. [Online]. Available: