Purification and some properties of an oxydative inhibitor in rabbit reticulocyte lysates

Erdogdu, G
Dholakia, JN
Wahba, AJ
Protein synthesis in rabbit reticulocyte lysates in the presence of heme is inhibited by 50% by the addition of 4 mM GSSG (oxidized glutathione). The incubation of the rabbit reticulocyte lysate with 4 mM GSSG at 30 degrees C for 30 min will cause activation of an inhibitor of protein synthesis which could be purified from the lysates through a five-step procedure. The inhibitor results in a 70-80% inhibition after alh incubation. The inhibitor consists of one polypeptide of 23 kDa apparent molecular weight and is 90% pure as judged by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. However, in the presence of cAMP (10 mM) or GEF (guanine nucleotide exchange factor) (0.3 mu g), protein synthesis in the inhibited reticulocyte lysate will be already recovered.


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In this study, immobilization of laccase (L) enzyme on magnetite (Fe(3)O(4)) nanoparticles was achieved, so that the immobilized enzyme could be used repeatedly. For this purpose, Fe(3)O(4) nanoparticles were coated and functionalized with chitosan (CS) and laccase from Trametes versicolor was immobilized onto chitosan-coated magnetic nanoparticles (Fe(3)O(4)-CS) by adsorption or covalent binding after activating the hydroxyl groups of chitosan with carbodiimide (EDAC) or cyanuric chloride (CC). For chitosa...
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TSapropterin Dihydrochloride is the synthetic form of tetrahydrobiopterin (BH4) which is cofactor of phenylalanine hydroxylase (PAH) enzyme. For people with phenylketonuria (PKU), oral administration of sapropterin dihydrochloride decrease phenylalanine level in blood by converting it to tyrosine. The purpose of this study is to develop an analytical method for the determination of this active ingredient in order to follow the production of the generic drug development of Kuvan®. Fourier-Transform infrared ...
ARINC, E; Güray, Nülüfer Tülün; SAPLAKOGLU, U; Adalı, Orhan (Elsevier BV, 1992-01-01)
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Abusafa, A; Yücel, Hayrettin (2002-08-01)
Distribution coefficients of cesium on natural and cation-enriched (Na(+), K(+), NH(4)(+) and Ca(+2)) forms of clinoptilolite were measured by batch, radioactive tracer technique. The measurements were carried out for an initial cesium concentration range of 10(-6)-10(-1) mol/dm(3) and at temperatures of 25, 40, 60 and 80 degreesC. Experimental isotherms evaluated from distribution coefficients were fit to Langmuir, Freundlich and Dubinin-Radushkevich (D-R)models. Of the models tested, D-R model was found t...
Citation Formats
G. Erdogdu, J. Dholakia, and A. Wahba, “Purification and some properties of an oxydative inhibitor in rabbit reticulocyte lysates,” ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES, pp. 897–901, 1998, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/66238.