Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Analysis and optimization of DNA delivery into chickpea (Cicer arietinum L.) seedlings by Agrobacterium tumefacience
Date
2008-04-17
Author
AKBULUT, Mikail
Yucel, Meral
Oktem, Hueseyin Avni
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
170
views
0
downloads
Cite This
The main purpose of this study was to develop a non-tissue culture based Agrobacterium mediated transformation method for chickpea. The influences of several factors were investigated on the transfer of -glucuronidase (GUS) gene into chickpea (Cicer arietinum) seedlings during the early stages of Agrobacterium-mediated gene transfer, including cocultivation period in liquid induction medium (2, 8, 16 and 24 h), strains of Agrobacterium tumefaciens (C58C1, EHA105, KYRT1) containing the plasmid pTJK136, developmental stage (16 h imbibed and 40 h germinated), microwounding, vacuum infiltration (200, 400, 600 mmHg for 20 and 40 min) and genotype (5 different). The number of GUS-expressing foci was counted to evaluate the gene transfer process. The KYRT1/pTJK136 strain of A. tumefaciens was significantly more effective for transformation than the C58C1/pTJK136 and EHA105/pTJK136 strains. The highest transient GUS activity was obtained from 16 h imbibed seedlings of cv.Uzunlu wounded with a needle and co-cultivated in liquid induction medium for 24 h with the KYRT1 strain (226 GUS foci/per explant).
Subject Keywords
Chickpea
,
Agrobacterium
,
Vacuum infiltration
,
Transient expression
URI
https://hdl.handle.net/11511/66756
Journal
AFRICAN JOURNAL OF BIOTECHNOLOGY
Collections
Department of Biology, Article
Suggestions
OpenMETU
Core
Optimization of selection conditions and agrobacterium mediated transformation of chickpea (cicer arietinum L. cv. Gökçe)
Öz, M. Tufan; Öktem, Hüseyin Avni; Department of Biotechnology (2005)
The objective of this study was to optimize an efficient selection system and Agrobacterium mediated transformation of chickpea (Cicer arietinum L.). Cotyledonary node explants of Turkish chickpea cultivar Gökçe were used to determine the effects of selective agents, two antibiotics (Kanamycin, Hygromycin) and two herbicides (PPT, Glyphosate) as well as four antibiotics (Augmentin, Carbenicillin, Cefotaxime, Timentin) for eliminating Agrobacterium on multiple shoot and root induction. Selective agents and a...
Optimization of mature embryo based regeneration and genetic transformation of Turkish wheat cultivars
Battal, Abdülhamit; Yücel, Ayşe Meral; Öktem, Hüseyin Avni; Department of Biotechnology (2010)
The objective of this study was to optimize tissue culture, transformation and regeneration parameters of mature embryo based culture of Triticum durum cv. Mirzabey 2000 and Triticum aestivum cv. Yüreğir 89. The effects of auxin type of hormone at different concentrations and dark incubation periods on regeneration capacity were evaluated. Two different hormone types 2,4- dichlorophenoxyacetic acid and picloram were used at three different concentrations 2, 4 and 8 mg/l. Mature embryo derived calli were inc...
Improved in vitro micropropagation method with adventitious corms and roots for endangered saffron
Zeybek, Evrim; Önde, Sertaç; Kaya, Zeki (2012-02-01)
The objective of this study was to investigate development of an efficient in vitro tissue culture system for saffron (Crocus sativus L.) complete with roots and corms. In indirect organogenesis, Murashige and Skoog (MS) media with 3% (w/v) sucrose, 100 mg L(-1) ascorbic acid, and the combination of 0.25 mg L(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg L(-1) 6-benzylaminopurine (BAP) were best for callus initiation and growth while 1.5 mg L(-1) BAP was excellent for high rate of adventitious shoot f...
Metabological engineering of Pichia Pastoris for extracellular thermostable glucose isomerase production
Ata, Özge; Çalık, Pınar; Özdamar, Tunçer H.; Department of Biotechnology (2012)
The aim of this study is to develop a metabolically engineered P. pastoris strain for production of an active extracellular thermostable glucose isomerase (GI) enzyme by using genetic engineering techniques. For this purpose, research program was performed in two sub-programs. In the first sub-program, xylA gene from Thermus thermophilus was amplified and inserted into pPICZα-A expression vector. Thereafter, this pPICZα-A::xylA vector was cloned into AOX1 locus in P. pastoris genome and expressed under alco...
Analysis of Antimicrobial and Coagulation Inducing Effects of a New Decontamination Powder
Tosun, Barış Çağrı; Öktem, Hüseyin Avni (2019-11-27)
In this study, the effect of a new formulation in the form of powder for the purpose of decontamination of skin from biological agents is investigated. Moreover, the formulation has also been shown to induce coagulation by erythrocyte aggregation, which is of significance since decontamination against B agents and bleeding wounds could be dealt with simultaneously by applying single formulation.
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
M. AKBULUT, M. Yucel, and H. A. Oktem, “Analysis and optimization of DNA delivery into chickpea (Cicer arietinum L.) seedlings by Agrobacterium tumefacience,”
AFRICAN JOURNAL OF BIOTECHNOLOGY
, pp. 1011–1017, 2008, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/66756.