Investigation into the effects of prenatal alcohol exposure on postnatal spine development and expression of synaptophysin and PSD95 in rat hippocampus

Elibol-Can, Birsen
Yuruker, Sinan
Jakubowska-Dogru, Ewa
Ethanol is known as a potent teratogen responsible for the fetal alcohol syndrome characterized by cognitive deficits especially pronounced in juveniles but ameliorating in adults. Since the mechanisms of these deficits and following partial recovery are not fully elucidated, the aim of the present study was to investigate the process of synaptogenesis in the hippocampus over the first two months of life in control and fetal-alcohol rats. Ethanol was delivered to the pregnant dams by intragastric intubation throughout 7-21 gestation days at the daily dose of 6 g/kg generating a mean blood alcohol level of 246.6 +/- 40.9 mg/dl on gestation day 20. The spine densities as well as the expression of pre- and postsynaptic proteins, synaptophysin (SYP) and PSD-95 protein, were evaluated for three distinct hippocampal regions: CA1, CA2+3, and DG and four postnatal days: PD1, PD10, PD30 and PD60, independently. Our results confirmed an intensive synaptogenesis within the brain spurt period (first 10 postnatal days), however, the temporal pattern of changes in the SYP and PSD-95 expression was different. The ethanol exposure during half of the 1st and the whole 2nd human trimester equivalent resulted in an overall trend toward lower values of synaptic indices at PD1 with a fast recovery from these deficits observed already at PD10. At PD30, around the age when the most pronounced behavioral deficits have been previously reported in juvenile fetal-alcohol subjects, no significant changes were found in either the hippocampal levels of synaptic proteins or in the spine density in principal hippocampal neurons. (C) 2013 ISDN. Published by Elsevier Ltd. All rights reserved.


Effects of prenatal binge-like ethanol exposure and maternal stress on postnatal morphological development of hippocampal neurons in rats
Jakubowska-Dogru, Ewa; Elibol, Birsen; Dursun, Ilknur; Yuruker, Sinan (Wiley, 2017-10-01)
Background: Alcohol is one of the most commonly used drugs of abuse negatively affecting human health and it is known as a potent teratogen responsible for fetal alcohol syndrome (FAS), which is characterized by cognitive deficits especially pronounced in juveniles but ameliorating in adults. Searching for the potential morphological correlates of these effects, in this study, we compared the course of developmental changes in the morphology of principal hippocampal neurons in fetal-alcohol (A group), intub...
Neuroprotective Efficacy of the Peroxisome Proliferator-Activated Receptor-gamma Ligand in Chronic Cerebral Hypoperfusion
SAYAN ÖZAÇMAK, HALE; SAYAN, Hale; BARUT, FİGEN; Jakubowska-Dogru, Ewa (Bentham Science Publishers Ltd., 2011-08-01)
Chronic cerebral hypoperfusion can cause learning and memory impairment and neuronal damage resembling the effects observed in vascular dementia. PPAR-gamma agonists were shown to modulate inflammatory response and neuronal death following cerebral ischemia. The present study was designed to evaluate possible neuroprotective effects of rosiglitazone, a PPAR-gamma agonist, in rat model of chronic cerebral hypoperfusion. Cerebral hypoperfusion was induced by permanent bilateral occlusion of the common carotid...
Development of newborn galactosemia screening kits /
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Galactosemia is an autosomal recessive disorder, caused by an impaired galactose metabolism, resulting in acute complications with high morbidity and mortality as soon as the infant begins a milk-based diet. Mutations in GALT, GALE and GLAK genes that are in the Leloir Pathway are responsible for the phenotype. Removing galactose from the diet is a critical, lifesaving intervention; yet this treatment does not prevent multiple long-term complications. Long-term neurological outcomes, particularly movement d...
Prenatal ethanol intoxication and maternal intubation stress alter cell survival and apoptosis in the postnatal development of rat hippocampus
ELİBOL, BİRSEN; BEKER, MERVE; Sahbaz, Cigdern Dilek; Kilic, Ulkan; Jakubowska-Dogru, Ewa (Exeley, Inc., 2019-01-01)
It is well known that the fetal ethanol exposure and prenatal stress may have adverse effects on brain development. Interestingly, some morphological and functional recovery from their teratogenic effects that take place during brain maturation. However, mechanisms that underlie this recovery are not fully elucidated. The aim of this study was to examine whether the postnatal attenuation of fetal alcohol - and maternal stress-induced morphological and functional deficits correlates with compensatory changes...
Investigation of hippocampal development during a protracted postnatal period in control and fetal alcohol wistar rats
Elibol Can, Birsen; Jakubowska Doğru, Ewa; Kılıç, Ertuğrul; Department of Biology (2013)
Behavioral deficits caused by fetal-alcohol are well expressed in juvenile subjects but usually ameliorate with maturation. It suggests some kind of postnatal regeneration. The aim of the present study was to examine the potential correlation between behavioral recovery and the postnatal hippocampal development in the fetal-alcohol rats. This study included behavioral tests applied to juvenile and adult subjects, unbiased stereology to investigate changes in neuron numbers and hippocampal volumes, the postn...
Citation Formats
B. Elibol-Can, E. KILIÇ, S. Yuruker, and E. Jakubowska-Dogru, “Investigation into the effects of prenatal alcohol exposure on postnatal spine development and expression of synaptophysin and PSD95 in rat hippocampus,” INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, pp. 106–114, 2014, Accessed: 00, 2020. [Online]. Available: