Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
In vitro evaluation of PLLA/PBS sponges as a promising biodegradable scaffold for neural tissue engineering
Date
2017-01-01
Author
Kanneci Altinisik, Irem Ayse
Kök, Fatma Neşe
Yucel, Deniz
KÖSE, GAMZE
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
306
views
0
downloads
Cite This
In tissue engineering, the use of poly-L-lactic acid (PLLA)/polybutylene succinate (PBS) blend for the construction of scaffold is very limited. Moreover, polymeric sponges fabricated from PLLA/PBS have not been studied for neural tissue engineering. In the present study, the potential of the utility of PLLA/PBS polymeric sponges seeded with Schwann cells was investigated. PLLA and PBS were blended in order to increase the processability and tune the crystallinity, porosity, and degradation rate of the resulted polymeric sponges. These sponges were then seeded with Schwann cells. Porosity analysis showed that there were no significant differences between different compositions of PLLA/PBS blends; however, the porosity was slightly higher in PLLA/PBS (3%, w/v, 2:1) scaffold. Degradation profiles were also investigated for 120 days and almost 25% weight of PLLA/PBS (6%, 4%, 2%, w/v, 1:1) scaffolds and 18% weight of PLLA/PBS (3%, w/v, 2:1) scaffolds were lost at the end of 120 days. In vitro cell culture studies were also performed and the results proved that all PLLA/PBS blended scaffolds were biocompatible. The highest cell proliferation was observed for PLLA/PBS (3%, w/v, 2:1) scaffolds and this construct can be considered a promising biodegradable scaffold for neural tissue engineering.
Subject Keywords
Genetics
,
Cell Biology
,
Physiology
,
Molecular Biology
,
General Agricultural and Biological Sciences
,
Microbiology
URI
https://hdl.handle.net/11511/67552
Journal
TURKISH JOURNAL OF BIOLOGY
DOI
https://doi.org/10.3906/biy-1701-6
Collections
Biomaterials and Tissue Engineering Application and Research Center (BİOMATEN), Article
Suggestions
OpenMETU
Core
Collagen/PEO/gold nanofibrous matrices for skin tissue engineering
Akturk, Omer; Keskin, Dilek (The Scientific and Technological Research Council of Turkey, 2016-01-01)
As a novel approach in skin tissue engineering, gold nanoparticles (AuNPs) were synthesized and incorporated at different concentrations into collagen/PEO nanofibrous matrices in this study. The group containing 14.27 ppm AuNPs (CM-Au) had the best nanofibrous morphology. CM-Au was cross-linked with glutaraldehyde vapor (CM-AuX). All groups were disrupted in collagenase in 2 h, but cross-linked groups and Matriderm (R) resisted hydrolytic degradation for 7 and 14 days, respectively. Due to its small pores a...
An in vitro human skeletal muscle model: coculture of myotubes, neuron-like cells, and the capillary network
Ertan, Ayse Burcu; KENAR, HALİME; Beyzadeoglu, Tahsin; Kök, Fatma Neşe; Kose, Gamze Torun (The Scientific and Technological Research Council of Turkey, 2017-01-01)
This study reports the generation of a new human muscle tissue equivalent from skeletal muscle-derived stem cells and human umbilical vein endothelial cells (HUVECs). Skeletal muscle stem cells were isolated by the preplate technique and differentiated into neuron-like cells that were positive for neuronal beta-tubulin3 and nestin and negative for the astrocyte marker glial fibrillary acidic protein (GFAP). Coculture of skeletal muscle stem cells with the HUVECs under optimized fetal bovine serum and media ...
In vivo examination of the effects of hydroxycinnamic acid on xenobiotic metabolizing and antioxidant enzymes
SEMİZ, ASLI; Celik-Turgut, Gurbet; KARAKURT, SERDAR; AKÇA, HAKAN; ARSLAN, ŞEVKİ; Adalı, Orhan; ŞEN, ALAATTİN (National Library of Serbia, 2017-01-01)
In the last decade, hydroxycinnamic acids (HCA) have gained increasing attention from researchers due to their antioxidant potential. The aim of this study was to examine in detail the impact of dietary HCA on particular types of P450 and also selected phase II and antioxidant enzymes in Wistar rat. HCA (10 mu M/kg/day, i.p.) was administered for ten continuous days. Examination of the activities and mRNA and protein levels revealed that CYP2B, 2C6 and 3A enzyme activities were not altered significantly, wi...
The effect of diabetes on rat skeletal muscle tissues at molecular level
Bozkurt, Özlem; Severcan, Feride; Department of Biology (2006)
In the present study Fourier Transform Infrared Spectroscopy was used to examine the effects of streptozotocin-induced diabetes mellitus on the structural components of slow- and fast-twitch rat skeletal muscles, at molecular level. Diabetes mellitus is a chronic disorder of carbohydrate, fat and protein metabolism, which is characterized by hyperglycemia caused by a defective or deficient insulin secretory response. The effect of diabetes is seen on a variety of tissues leading to important secondary compl...
A promising enzyme anchoring probe for selective ethanol sensing in beverages
Soylemez, Saniye; Goker, Seza; Toppare, Levent Kamil (Elsevier BV, 2019-07-15)
A newly designed amperometric biosensor for the determination of ethanol through one-step electrochemical coating of (4,7-di(thiophen-2-yl)benzo[c][1,2,5]selenadiazole-co-1H-pyrrole-3-carboxylic acid) (TBeSe-co-P3CA) on a graphite electrode is presented. It was aimed to propose a newly synthesized copolymer with enhanced biosensing properties as a novel sensor for the quantification of ethanol. The conjugated copolymer (TBeSe-co-P3CA) was prepared through electrochemical polymerization by potential cycling....
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
I. A. Kanneci Altinisik, F. N. Kök, D. Yucel, and G. KÖSE, “In vitro evaluation of PLLA/PBS sponges as a promising biodegradable scaffold for neural tissue engineering,”
TURKISH JOURNAL OF BIOLOGY
, pp. 734–747, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/67552.