Immunoaffinity Column Cleanup with Liquid Chromatography Using Postcolumn Bromination for the Determination of Aflatoxins in Black and White Sesame Seed: Single-Laboratory Validation

2012-01-01
Liu, Guihua
Zhu, Zhou
Cheng, Jinquan
Senyuva, Hamide Z.
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A single-laboratory validation was conducted to establish the effectiveness of an immunoaffinity column cleanup procedure followed by LC with fluorescence detection for the determination of aflatoxins B-1, B-2, G(1), and G(2) in sesame seeds. The sample is homogenized with 50% water (w/w) to form a slurry, then the test portion is extracted with methanol-water (60 + 40, v/v) using a high-speed blender. The sample extract is filtered, diluted with 15% Tween 20 in phosphate-buffered saline solution, and applied to an immunoaffinity column. Aflatoxins are removed with neat methanol, then directly determined by RP-LC with fluorescence detection using postcolumn bromination (Kobra cell). Test portions of blank white sesame seed slurry were spiked with a mixture of aflatoxins to give total levels of 4 and 10 mu g/kg. Recoveries for individual and total aflatoxins ranged from 92.7 to 110.3% for spiked samples. Based on results for spiked sesame paste (triplicates at two levels), the RSD for repeatability (RSDr) averaged 1.1% for total aflatoxins and 1.4% for aflatoxin B-1. The method was demonstrated to be applicable to naturally contaminated samples of black and white sesame seeds obtained from local markets in China.
Agronomy and Crop Science, Food Science, Analytical Chemistry, Pharmacology, Environmental Chemistry
https://hdl.handle.net/11511/67800
JOURNAL OF AOAC INTERNATIONAL
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Citation Formats
G. Liu, Z. Zhu, J. Cheng, and H. Z. Senyuva, “Immunoaffinity Column Cleanup with Liquid Chromatography Using Postcolumn Bromination for the Determination of Aflatoxins in Black and White Sesame Seed: Single-Laboratory Validation,” JOURNAL OF AOAC INTERNATIONAL, pp. 122–128, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/67800.
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