Advances in CRISPR/Cas9 Technology for in Vivo Translation

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2019-03-01

Author

Cicek, Yagiz Anil
Luther, David C.
Kretzmann, Jessica A.
Rotello, Vincent M.

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9 (Cas9) technology has revolutionized therapeutic gene editing by providing researchers with a new method to study and cure diseases previously considered untreatable. While the full range and power of CRISPR technology for therapeutics is being elucidated through in vitro studies, translation to in vivo studies is slow. To date there is no totally effective delivery strategy to carry CRISPR components to the target site in vivo. The complexity of in vivo delivery is furthered by the number of potential delivery methods, the different forms in which CRISPR can be delivered as a therapeutic, and the disease target and tissue type in question. There are major challenges and limitations to delivery strategies, and it is imperative that future directions are guided by well-conducted studies that consider the full effect these variables have on the eventual outcome. In this review we will discuss the advances of the latest in vivo CRISPR/Cas9 delivery strategies and highlight the challenges yet to be overcome.

Subject Keywords

Pharmacology, Pharmaceutical Science, General Medicine

URI

https://hdl.handle.net/11511/67829

Journal

BIOLOGICAL & PHARMACEUTICAL BULLETIN

DOI

https://doi.org/10.1248/bpb.b18-00811

Collections

Department of Chemistry, Article

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Citation Formats

Y. A. Cicek, D. C. Luther, J. A. Kretzmann, and V. M. Rotello, “Advances in CRISPR/Cas9 Technology for in Vivo Translation,” BIOLOGICAL & PHARMACEUTICAL BULLETIN, pp. 304–311, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/67829.